Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   AW22_RS04215 Genome accession   NZ_CP009300
Coordinates   568196..568630 (+) Length   144 a.a.
NCBI ID   WP_000811492.1    Uniprot ID   A0A0G8EA73
Organism   Bacillus cereus D17     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 563196..573630
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AW22_RS04195 (AW22_610) - 564085..565248 (-) 1164 WP_042512223.1 aminotransferase A -
  AW22_RS04200 (AW22_611) - 565371..565571 (-) 201 WP_000929284.1 hypothetical protein -
  AW22_RS04205 (AW22_612) kinB 565882..567156 (+) 1275 WP_000420389.1 sporulation sensor histidine kinase KinB -
  AW22_RS04210 (AW22_613) - 567210..567959 (-) 750 WP_000388464.1 DUF3967 domain-containing protein -
  AW22_RS04215 (AW22_614) nucA/comI 568196..568630 (+) 435 WP_000811492.1 DNA-entry nuclease Machinery gene
  AW22_RS04220 (AW22_615) comJ 568667..569056 (+) 390 WP_001995060.1 competence protein ComJ -
  AW22_RS04225 (AW22_616) metE 569093..571381 (-) 2289 WP_001007632.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  AW22_RS04235 (AW22_617) - 572309..572866 (-) 558 WP_000062080.1 PadR family transcriptional regulator -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16012.35 Da        Isoelectric Point: 8.4331

>NTDB_id=128350 AW22_RS04215 WP_000811492.1 568196..568630(+) (nucA/comI) [Bacillus cereus D17]
MKQLKGIIISIIAILSILVAVYEVLVPEETSTKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=128350 AW22_RS04215 WP_000811492.1 568196..568630(+) (nucA/comI) [Bacillus cereus D17]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTTTAGTAGCGGTTTATGAAGTACTTGTTCC
AGAGGAAACAAGTACAAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGAGATATCCAGAAACAGGGA
AACATATTACGGATGCGATAAAGGAAGGACATTCAGAAGTTTGTACAATCGACCGTGGTGGTGCTGCGGATAGAAGAAAG
TTATCGTTAGCTCCGTACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACATATCGAATATATAAGTCCAGCAGATAACCGCGGAGCAGGGTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G8EA73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

70.796

78.472

0.556

Multiple sequence alignment