Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FORC14_RS14455 Genome accession   NZ_CP011406
Coordinates   3091308..3093032 (-) Length   574 a.a.
NCBI ID   WP_015297335.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain FORC_014     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3086308..3098032
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC14_RS14420 (FORC14_2816) - 3086641..3087354 (-) 714 WP_063517785.1 PilW family protein -
  FORC14_RS14425 (FORC14_2817) - 3087354..3087953 (-) 600 WP_005497286.1 type II secretion system protein -
  FORC14_RS14430 (FORC14_2818) - 3087950..3088429 (-) 480 WP_063517786.1 type II secretion system protein -
  FORC14_RS14435 (FORC14_2819) - 3088545..3088778 (-) 234 WP_063517787.1 type II secretion system protein -
  FORC14_RS14440 (FORC14_2820) - 3088846..3089316 (-) 471 WP_025498813.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FORC14_RS14445 (FORC14_2821) - 3089611..3090072 (-) 462 WP_025517825.1 MSHA biogenesis protein MshF -
  FORC14_RS14450 (FORC14_2822) - 3090076..3091299 (-) 1224 WP_063517788.1 type II secretion system F family protein -
  FORC14_RS14455 (FORC14_2823) pilB 3091308..3093032 (-) 1725 WP_015297335.1 GspE/PulE family protein Machinery gene
  FORC14_RS14460 (FORC14_2824) - 3093029..3094105 (-) 1077 WP_161610653.1 tetratricopeptide repeat protein -
  FORC14_RS14465 (FORC14_2825) - 3094102..3094947 (-) 846 WP_005497278.1 ExeA family protein -
  FORC14_RS14470 (FORC14_2826) mshL 3094991..3096634 (-) 1644 WP_005456314.1 pilus (MSHA type) biogenesis protein MshL -
  FORC14_RS14475 (FORC14_2827) - 3096655..3096984 (-) 330 WP_005481006.1 MSHA biogenesis protein MshK -
  FORC14_RS14480 (FORC14_2828) gspM 3096977..3097627 (-) 651 WP_005497276.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63902.21 Da        Isoelectric Point: 5.2324

>NTDB_id=127873 FORC14_RS14455 WP_015297335.1 3091308..3093032(-) (pilB) [Vibrio parahaemolyticus strain FORC_014]
MKIQLRKRLGDLLVEEGIVSEDQIQQALSAQRSTGQKLGDALIDLGFITEKQMLEFLSQQLGLPLIDLGRAPVDADAVQI
LPEVHARRLRAMVVARNGDTLRVAMSDPADLFTQESLMNLLGEYNLEFIIASERQLISSFDRYYRRTKEIASFAEQLQAE
HQDVQSFDYGIDEADSEEVTVVKLVNSMFEDAVQVGASDIHIEPDDKVLRLRQRVDGVLHETILNEVNIASALVLRLKLM
AHLDISEKRLPQDGRFNIKVRGQSIDIRMSTLPTQYGESVVMRLLNQSSGLRPLEESGLPPELLSRLRRQLSRPHGMILV
TGPTGSGKTTTLYGALSELNEPGKKIITAEDPVEYRLPRITQVQINSKIDLTFSRVLRTFLRQDPDIILIGEMRDQETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTQDHLDESRQQWLAGRFPNQV
GVTFHKGAGCQNCNLTGYRGRIGVFEMLELEHEMMDKLRANDAVGFAQAARRSENYKPLLASAMELALQGAVSLDEVMTL
GEGDASGKTDPIFM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=127873 FORC14_RS14455 WP_015297335.1 3091308..3093032(-) (pilB) [Vibrio parahaemolyticus strain FORC_014]
ATGAAAATTCAGTTGAGAAAACGCTTAGGTGACCTACTCGTTGAAGAAGGCATCGTATCGGAAGATCAAATCCAGCAAGC
TCTATCCGCTCAGCGCAGTACCGGACAAAAATTAGGTGATGCCCTGATTGACCTCGGGTTTATTACCGAAAAGCAAATGC
TGGAGTTCTTGTCTCAGCAACTAGGATTACCGTTGATCGATCTTGGACGCGCTCCAGTGGATGCGGATGCGGTGCAAATA
CTGCCAGAAGTGCATGCTCGTCGTTTACGTGCAATGGTGGTGGCTCGTAATGGTGACACTTTACGTGTTGCGATGAGTGA
TCCGGCGGATCTGTTTACCCAAGAGTCTTTAATGAACTTGCTAGGTGAGTACAACCTTGAGTTCATCATTGCCTCTGAGC
GTCAGTTGATCAGCAGTTTCGACCGTTACTACCGCCGTACTAAAGAGATCGCGTCCTTTGCTGAGCAGTTACAAGCCGAG
CACCAAGACGTACAAAGCTTTGACTATGGTATCGATGAAGCTGACAGTGAAGAAGTTACCGTTGTAAAACTGGTTAATTC
GATGTTTGAGGACGCGGTGCAAGTTGGTGCATCGGATATCCATATCGAACCAGACGATAAAGTCCTGCGTTTACGCCAGC
GTGTCGACGGTGTGCTACATGAAACCATTCTGAACGAAGTGAACATCGCTTCAGCATTGGTATTACGCCTTAAGTTGATG
GCGCACTTGGATATTTCTGAGAAGCGTCTGCCACAAGATGGTCGCTTCAACATCAAAGTTCGTGGTCAATCGATTGATAT
TCGTATGTCGACGTTACCGACTCAGTACGGTGAATCGGTCGTAATGCGTCTGCTTAACCAGTCGTCAGGTTTACGTCCAT
TAGAAGAATCGGGTTTACCGCCTGAGCTACTGTCGCGCTTGCGTCGTCAGTTATCTCGTCCTCATGGCATGATTTTGGTA
ACCGGCCCTACGGGATCGGGTAAAACCACGACACTTTACGGTGCGTTGAGTGAGCTCAATGAGCCGGGTAAAAAGATCAT
CACCGCAGAAGATCCGGTGGAATACCGTTTACCACGTATCACGCAAGTACAGATCAACAGCAAGATCGACCTGACATTTT
CACGCGTGCTACGCACCTTCTTACGCCAAGACCCAGACATCATTTTGATTGGTGAGATGCGCGACCAAGAGACGGTAGAG
ATTGGCTTGCGAGCAGCACTAACCGGCCACTTAGTATTGAGTACGTTGCACACCAACGATGCGGTCGACAGCGCACTGCG
TATGATCGACATGGGAGCGCCAGGCTATCTCGTTGCCAGTGCCGTGCGTGCCGTTGTGGCTCAGCGATTGGTTCGCCGTG
TTTGCCCAGATTGTAAAACCCAAGATCATCTCGATGAGTCGCGTCAACAATGGTTAGCGGGGCGTTTTCCAAATCAAGTT
GGGGTGACTTTCCACAAAGGAGCAGGTTGTCAAAACTGTAACTTGACGGGGTATCGAGGTCGAATCGGTGTGTTTGAAAT
GCTTGAGCTGGAACATGAGATGATGGACAAGCTGCGTGCGAATGATGCGGTTGGCTTCGCACAAGCGGCTCGACGTTCAG
AAAACTACAAACCGCTTCTTGCCTCAGCAATGGAGCTTGCGCTGCAAGGTGCTGTGAGCCTGGATGAAGTGATGACCCTC
GGCGAGGGTGACGCTTCGGGTAAAACTGATCCCATTTTCATGTAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(187-453)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.64

97.387

0.376
  pilB/pilB1 Synechocystis sp. PCC 6803

34.272

100

0.361