Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   VV93_RS13830 Genome accession   NZ_CP009261
Coordinates   3003782..3004789 (-) Length   335 a.a.
NCBI ID   WP_011151279.1    Uniprot ID   -
Organism   Vibrio vulnificus strain 93U204     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2998782..3009789
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VV93_RS13805 (VV93_v1c27180) aroK 2999599..3000117 (-) 519 WP_011079357.1 shikimate kinase AroK -
  VV93_RS13810 (VV93_v1c27190) pilQ 3000319..3002103 (-) 1785 WP_011151276.1 type IV pilus secretin PilQ Machinery gene
  VV93_RS13815 (VV93_v1c27200) pilP 3002117..3002629 (-) 513 WP_011079355.1 pilus assembly protein PilP Machinery gene
  VV93_RS13820 (VV93_v1c27210) pilO 3002619..3003209 (-) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  VV93_RS13825 (VV93_v1c27220) pilN 3003202..3003792 (-) 591 WP_011079353.1 PilN domain-containing protein Machinery gene
  VV93_RS13830 (VV93_v1c27230) pilM 3003782..3004789 (-) 1008 WP_011151279.1 type IV pilus assembly protein PilM Machinery gene
  VV93_RS13835 (VV93_v1c27240) - 3004929..3007442 (+) 2514 WP_039554841.1 penicillin-binding protein 1A -
  VV93_RS13840 (VV93_v1c27250) oxyR 3007513..3008412 (-) 900 WP_039554843.1 DNA-binding transcriptional regulator OxyR -
  VV93_RS13845 (VV93_v1c27260) - 3008686..3008958 (+) 273 WP_011079349.1 DUF3624 domain-containing protein -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 37638.05 Da        Isoelectric Point: 6.3873

>NTDB_id=127828 VV93_RS13830 WP_011151279.1 3003782..3004789(-) (pilM) [Vibrio vulnificus strain 93U204]
MGKPFVTGIDIGHRSLKAVILKPYGEHYALLGYKEIIIKDGIFSDNHTLNHQEIVNSLKELKKELPLFRYQVALAVPDSS
VISKMLSIDAELDGREREFAILQAFAHQSPFPVDELSIDYVQLVEETSKTNHSYQVYATKKEVVESRVRVAEEVGLKPVL
VDVHAHSLCNILTLAAERHPDKASYCLLDVGYDTTSLVMSRRNEPPLVKEFAYGVSHLNASIGDDLTSPDAHEKTDIFNK
ELTERVRRQLQLFQSMDSQSKVNGLWICGEGAITPLLSETLQRKLSLECEVLNPLALFEQKTAKRKRRIVDWHHFSVAAG
LAIGGIQWLEDSHAA

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=127828 VV93_RS13830 WP_011151279.1 3003782..3004789(-) (pilM) [Vibrio vulnificus strain 93U204]
ATGGGTAAGCCGTTCGTTACCGGAATTGATATTGGTCATCGTAGCCTCAAGGCTGTCATTCTGAAACCTTATGGAGAGCA
CTACGCCCTGTTAGGGTACAAAGAAATAATCATTAAAGACGGTATTTTCTCAGATAACCACACTCTGAATCATCAAGAAA
TTGTCAATAGCCTGAAAGAACTGAAGAAGGAACTGCCTCTTTTCCGTTATCAGGTGGCACTGGCGGTGCCTGATAGTTCA
GTGATTAGTAAGATGTTATCGATCGATGCGGAGTTGGATGGTCGAGAGCGAGAATTCGCTATCCTTCAGGCGTTTGCACA
TCAGTCGCCGTTTCCTGTCGATGAATTATCGATTGATTATGTCCAATTGGTCGAAGAAACCAGCAAAACGAATCATAGCT
ATCAAGTTTACGCCACCAAAAAAGAGGTGGTGGAAAGCCGTGTGCGTGTCGCAGAAGAAGTGGGACTGAAACCCGTGCTG
GTAGACGTGCATGCGCATAGCCTGTGCAACATCTTAACGTTAGCGGCAGAGCGCCATCCAGATAAAGCCAGCTATTGCTT
GTTGGATGTGGGTTATGACACCACCTCTTTGGTGATGTCGCGTCGCAACGAACCGCCTTTAGTTAAAGAATTTGCCTATG
GGGTGTCTCACCTCAATGCGTCGATAGGCGACGATCTGACTTCTCCTGATGCTCATGAGAAAACCGACATCTTTAATAAA
GAACTGACAGAGCGAGTTCGTCGGCAATTGCAGCTGTTCCAATCTATGGATAGCCAAAGCAAAGTGAATGGCCTCTGGAT
CTGCGGTGAAGGCGCGATTACTCCGTTGTTGAGTGAAACGCTGCAGAGAAAGCTGAGTTTGGAGTGCGAAGTCCTTAATC
CTCTGGCTCTGTTTGAGCAAAAAACGGCGAAAAGAAAGCGCCGTATTGTTGATTGGCACCATTTTTCGGTGGCAGCGGGA
TTAGCGATTGGTGGCATTCAGTGGCTGGAGGATAGCCATGCAGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

53.433

100

0.534
  pilM Vibrio cholerae strain A1552

49.267

100

0.501

Multiple sequence alignment