Detailed information    

experimental Experimentally validated

Overview

Name   djlA   Type   Unclear
Locus tag   LPP_RS11585 Genome accession   NC_006368
Coordinates   2630060..2630950 (+) Length   296 a.a.
NCBI ID   WP_014844489.1    Uniprot ID   A0A3A6UFI9
Organism   Legionella pneumophila str. Paris     
Function   require for natural transformation   
Unclear

Function

Partial transformation defects were observed for mutants of djlA.


Genomic Context

Location: 2625060..2635950
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPP_RS11560 (lpp2284) prfA 2625219..2626307 (+) 1089 WP_010948042.1 peptide chain release factor 1 -
  LPP_RS11565 (lpp2285) prmC 2626300..2627163 (+) 864 WP_014844485.1 peptide chain release factor N(5)-glutamine methyltransferase -
  LPP_RS11570 (lpp2286) dksA 2627307..2627738 (+) 432 WP_014844486.1 RNA polymerase-binding protein DksA -
  LPP_RS11575 (lpp2287) - 2627808..2628641 (+) 834 WP_015961433.1 alpha/beta hydrolase -
  LPP_RS11580 (lpp2288) waaA 2628646..2629905 (-) 1260 WP_014844488.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  LPP_RS11585 (lpp2289) djlA 2630060..2630950 (+) 891 WP_014844489.1 co-chaperone DjlA Unclear
  LPP_RS11590 (lpp2290) - 2630917..2631690 (-) 774 WP_014844490.1 DUF3530 family protein -
  LPP_RS11595 (lpp2291) plaA 2631882..2632811 (-) 930 WP_014844491.1 GDSL family lysophospholipase PlaA -
  LPP_RS11600 (lpp2292) mavE 2633081..2633596 (+) 516 WP_011947187.1 Dot/Icm T4SS effector MavE -
  LPP_RS11605 (lpp2293) - 2633753..2633971 (-) 219 WP_015961434.1 hypothetical protein -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 34114.96 Da        Isoelectric Point: 9.7683

>NTDB_id=1277 LPP_RS11585 WP_014844489.1 2630060..2630950(+) (djlA) [Legionella pneumophila str. Paris]
MNLRDFFVITTWWGKILGAFFGYLTAGPVGALFGILVGNFFDRGLVSYYSNPHWLYHAEKQRIVQKAFFEATFSIMGHVA
KSDGRVSEQEISMAKSIMNEMKLSKGQKDLAKRLFNEGKQPDFNVSLALIQLQRICKDNRDLLKLFVDIQYRAAQVDGLS
SQKIHALDNIFTHLGFAPLHKQYRFYEDFGSYFQQEQSKQHYHNQQGYKHTSSSQDQQGYKPQSPPNTLAHAFALLEVSP
NASKQEVRRAYRRLLSRNHPDKLIAQGLPEEMIKLANDKTHQIMKAYELICETKGW

Nucleotide


Download         Length: 891 bp        

>NTDB_id=1277 LPP_RS11585 WP_014844489.1 2630060..2630950(+) (djlA) [Legionella pneumophila str. Paris]
ATGAACTTACGTGATTTCTTTGTAATAACAACCTGGTGGGGAAAAATACTTGGTGCCTTCTTTGGCTACTTAACTGCTGG
ACCAGTAGGCGCTTTGTTTGGCATTCTCGTTGGAAACTTTTTTGATCGGGGCCTTGTCAGTTATTACTCTAATCCCCATT
GGCTGTACCATGCAGAAAAACAAAGAATCGTTCAAAAAGCTTTCTTTGAAGCCACTTTCTCCATAATGGGGCATGTTGCG
AAATCGGATGGACGTGTTTCTGAACAAGAGATTAGCATGGCTAAATCCATCATGAATGAAATGAAATTGAGCAAGGGACA
AAAAGATTTAGCAAAACGATTATTTAATGAAGGGAAACAACCTGATTTCAATGTCAGCCTTGCTCTTATCCAATTACAAA
GAATCTGCAAGGATAACCGTGATTTGTTAAAGCTTTTTGTAGATATTCAGTATAGGGCAGCTCAAGTAGATGGATTAAGT
AGCCAAAAAATTCATGCGCTAGATAACATTTTTACGCATCTGGGATTTGCTCCCTTACACAAGCAGTATCGTTTTTATGA
GGATTTTGGCTCTTATTTCCAGCAAGAACAATCCAAACAACACTATCATAATCAACAGGGGTACAAGCATACCTCCTCTT
CTCAAGATCAACAGGGTTACAAACCTCAATCACCCCCTAATACTTTAGCGCACGCTTTTGCATTGCTTGAGGTAAGTCCG
AATGCAAGCAAACAGGAGGTTAGAAGAGCTTACAGACGCCTATTGAGCCGTAACCATCCAGATAAATTAATTGCTCAAGG
TTTACCCGAAGAAATGATTAAATTGGCTAACGATAAAACTCATCAAATTATGAAAGCCTATGAATTGATCTGTGAAACCA
AGGGCTGGTAG

Domains

Predicted by InterproScan.

DnaJ domain (DnaJ)

(233-291)

Tellurite resistance protein TerB (TerB)

(71-158)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3A6UFI9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  djlA Legionella pneumophila strain ERS1305867

100

100

1

Multiple sequence alignment    



References

[1] Léo Hardy et al. (2021) Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation. Journal of Bacteriology 203(3):e00548-20. [PMID: 33168636]