Detailed information    

experimental Experimentally validated

Overview

Name   djlA   Type   Unclear
Locus tag   GCO85_RS11730 Genome accession   NZ_CP048618
Coordinates   2634284..2635174 (+) Length   296 a.a.
NCBI ID   WP_014844489.1    Uniprot ID   A0A3A6UFI9
Organism   Legionella pneumophila strain ERS1305867     
Function   require for natural transformation   
Unclear

Function

Partial transformation defects were observed for mutants of djlA.


Genomic Context

Location: 2629284..2640174
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCO85_RS11705 (GCO85_11715) prfA 2629443..2630531 (+) 1089 WP_042755102.1 peptide chain release factor 1 -
  GCO85_RS11710 (GCO85_11720) prmC 2630524..2631387 (+) 864 WP_027225915.1 peptide chain release factor N(5)-glutamine methyltransferase -
  GCO85_RS11715 (GCO85_11725) dksA 2631486..2631962 (+) 477 WP_010948044.1 RNA polymerase-binding protein DksA -
  GCO85_RS11720 (GCO85_11730) - 2632032..2632865 (+) 834 WP_015961433.1 alpha/beta hydrolase -
  GCO85_RS11725 (GCO85_11735) waaA 2632870..2634129 (-) 1260 WP_061636230.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  GCO85_RS11730 (GCO85_11740) djlA 2634284..2635174 (+) 891 WP_014844489.1 co-chaperone DjlA Unclear
  GCO85_RS11735 (GCO85_11745) - 2635141..2635914 (-) 774 WP_027225918.1 DUF3530 family protein -
  GCO85_RS11740 (GCO85_11750) - 2636106..2637035 (-) 930 WP_014844491.1 SGNH/GDSL hydrolase family protein -
  GCO85_RS11745 (GCO85_11755) mavE 2637305..2637820 (+) 516 WP_011947187.1 Dot/Icm T4SS effector MavE -
  GCO85_RS11750 (GCO85_11760) - 2637977..2638195 (-) 219 WP_015961434.1 hypothetical protein -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 34114.96 Da        Isoelectric Point: 9.7683

>NTDB_id=1264 GCO85_RS11730 WP_014844489.1 2634284..2635174(+) (djlA) [Legionella pneumophila strain ERS1305867]
MNLRDFFVITTWWGKILGAFFGYLTAGPVGALFGILVGNFFDRGLVSYYSNPHWLYHAEKQRIVQKAFFEATFSIMGHVA
KSDGRVSEQEISMAKSIMNEMKLSKGQKDLAKRLFNEGKQPDFNVSLALIQLQRICKDNRDLLKLFVDIQYRAAQVDGLS
SQKIHALDNIFTHLGFAPLHKQYRFYEDFGSYFQQEQSKQHYHNQQGYKHTSSSQDQQGYKPQSPPNTLAHAFALLEVSP
NASKQEVRRAYRRLLSRNHPDKLIAQGLPEEMIKLANDKTHQIMKAYELICETKGW

Nucleotide


Download         Length: 891 bp        

>NTDB_id=1264 GCO85_RS11730 WP_014844489.1 2634284..2635174(+) (djlA) [Legionella pneumophila strain ERS1305867]
ATGAACTTACGTGATTTCTTTGTAATAACAACCTGGTGGGGAAAAATACTTGGTGCCTTCTTTGGCTACTTAACTGCTGG
ACCAGTAGGCGCTTTGTTTGGCATTCTCGTTGGAAACTTTTTTGATCGGGGCCTTGTCAGTTATTACTCTAATCCCCATT
GGCTGTACCATGCAGAAAAACAAAGAATCGTTCAAAAAGCTTTCTTTGAAGCCACTTTCTCCATAATGGGGCATGTTGCG
AAATCGGATGGACGTGTTTCTGAACAAGAGATTAGCATGGCTAAATCCATCATGAATGAAATGAAATTGAGCAAGGGACA
AAAAGATTTGGCAAAACGATTATTTAATGAAGGGAAACAACCTGATTTCAATGTCAGCCTTGCTCTTATCCAATTACAAA
GAATCTGCAAGGATAACCGTGATTTGTTAAAGCTTTTTGTAGATATTCAGTATAGGGCAGCTCAAGTAGATGGATTAAGT
AGCCAAAAAATTCATGCGCTAGATAACATTTTTACGCATCTGGGATTTGCTCCCTTACACAAGCAGTATCGTTTTTATGA
GGATTTTGGCTCTTATTTCCAGCAAGAACAATCCAAACAACACTATCATAATCAACAGGGGTACAAGCATACCTCCTCTT
CTCAAGATCAACAGGGTTACAAACCTCAATCACCCCCTAATACTTTAGCGCACGCTTTTGCATTGCTTGAGGTAAGTCCG
AATGCAAGCAAACAGGAGGTTAGAAGAGCTTACAGACGCCTATTGAGCCGTAACCATCCAGATAAATTAATTGCTCAAGG
TTTACCCGAAGAAATGATTAAATTGGCTAACGATAAAACTCATCAAATTATGAAAGCCTATGAATTGATCTGTGAAACCA
AGGGCTGGTAG

Domains

Predicted by InterproScan.

DnaJ domain (DnaJ)

(233-291)

Tellurite resistance protein TerB (TerB)

(71-158)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3A6UFI9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  djlA Legionella pneumophila str. Paris

100

100

1

Multiple sequence alignment    



References

[1] Léo Hardy et al. (2021) Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation. Journal of Bacteriology 203(3):e00548-20. [PMID: 33168636]