Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   IX88_RS04740 Genome accession   NZ_CP009256
Coordinates   999287..1000087 (+) Length   266 a.a.
NCBI ID   WP_187406181.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain AB31     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 994287..1005087
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IX88_RS04715 (IX88_04775) - 995197..996495 (+) 1299 WP_144242547.1 glycosyltransferase family 1 protein -
  IX88_RS04720 (IX88_04780) - 996520..997071 (+) 552 WP_000669571.1 phosphatase PAP2 family protein -
  IX88_RS04725 (IX88_04785) iscX 997171..997371 (+) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  IX88_RS04730 (IX88_04790) ndk 997485..997916 (+) 432 WP_031999785.1 nucleoside-diphosphate kinase -
  IX88_RS04735 (IX88_04795) rlmN 998039..999271 (+) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  IX88_RS04740 (IX88_04800) pilF 999287..1000087 (+) 801 WP_187406181.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  IX88_RS04745 (IX88_04805) - 1000078..1000869 (+) 792 WP_000405163.1 RodZ domain-containing protein -
  IX88_RS04750 (IX88_04810) ispG 1000891..1002006 (+) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  IX88_RS04755 (IX88_04815) hisS 1002003..1003295 (+) 1293 WP_000095254.1 histidine--tRNA ligase -
  IX88_RS04760 (IX88_04820) - 1003324..1004022 (+) 699 WP_000058788.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30018.39 Da        Isoelectric Point: 9.3928

>NTDB_id=127671 IX88_RS04740 WP_187406181.1 999287..1000087(+) (pilF) [Acinetobacter baumannii strain AB31]
MSTPKLKTMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQLRIAGATLGYDQRYQALVNLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=127671 IX88_RS04740 WP_187406181.1 999287..1000087(+) (pilF) [Acinetobacter baumannii strain AB31]
TTGAGTACTCCGAAGTTGAAGACCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCCAGTGGTTGCCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCAGAACATTACTTTAAGCGTGCAATTTCATCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAACTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGTAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

98.872

100

0.989
  pilF Acinetobacter baylyi ADP1

74.615

97.744

0.729

Multiple sequence alignment