Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   AZ49_RS06530 Genome accession   NZ_CP009240
Coordinates   1394688..1395632 (+) Length   314 a.a.
NCBI ID   WP_014017056.1    Uniprot ID   G0VLY9
Organism   Megasphaera elsdenii 14-14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1389688..1400632
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AZ49_RS06520 (AZ49_06585) - 1389931..1390884 (-) 954 WP_014017058.1 magnesium transporter CorA family protein -
  AZ49_RS06525 (AZ49_06590) smc 1391131..1394685 (+) 3555 WP_054336364.1 chromosome segregation protein SMC -
  AZ49_RS06530 (AZ49_06595) pilA 1394688..1395632 (+) 945 WP_014017056.1 signal recognition particle-docking protein FtsY Machinery gene
  AZ49_RS06535 (AZ49_06600) - 1395644..1396108 (+) 465 WP_054336365.1 YhcH/YjgK/YiaL family protein -
  AZ49_RS06540 (AZ49_06605) thiM 1396123..1396935 (+) 813 WP_014017054.1 hydroxyethylthiazole kinase -
  AZ49_RS06545 (AZ49_06610) thiW 1396928..1397431 (+) 504 WP_014017053.1 energy coupling factor transporter S component ThiW -
  AZ49_RS06550 (AZ49_06615) - 1397565..1398155 (+) 591 WP_022496855.1 TetR/AcrR family transcriptional regulator -
  AZ49_RS06555 (AZ49_06620) - 1398158..1399330 (+) 1173 WP_054336366.1 efflux RND transporter periplasmic adaptor subunit -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 33780.64 Da        Isoelectric Point: 4.7563

>NTDB_id=127514 AZ49_RS06530 WP_014017056.1 1394688..1395632(+) (pilA) [Megasphaera elsdenii 14-14]
MGFFSKLRKGLEKTKHSLIQNIETVVRGYAKIDDDMYDDLEAVMLTGDIGVETTEYLLDQIRTGVKSKEIKDGNDVVPYL
EKCIVALLNENDEPLPETEGPRVIFIVGVNGVGKTTTIGKLAKYYKQQGKSVMLAAGDTFRAAASEQLTIWADRVGVPIV
KHQEGADAAAVVFDATASAKAKGMDILLVDTAGRLQTKSNLMEELRKMARVAGRNIEGAPQETLLVLDATTGQNAVSQAK
LFGDVVPLTGVVLTKLDGTAKGGVILSVKRELGVPVRWVGVGEGVDDLRPFDAAQFADALFDKNVAAREASDED

Nucleotide


Download         Length: 945 bp        

>NTDB_id=127514 AZ49_RS06530 WP_014017056.1 1394688..1395632(+) (pilA) [Megasphaera elsdenii 14-14]
ATGGGATTTTTTTCAAAACTTCGAAAAGGCTTAGAAAAGACGAAACACTCGCTCATCCAGAATATCGAAACCGTCGTCCG
CGGCTATGCCAAAATAGACGACGACATGTACGATGACCTGGAAGCGGTCATGCTCACCGGAGACATCGGCGTCGAAACGA
CGGAGTATCTCCTGGACCAGATACGGACCGGCGTCAAATCGAAGGAAATCAAAGACGGCAACGACGTCGTACCGTATTTG
GAAAAATGCATCGTCGCCTTGCTCAATGAAAACGATGAACCCCTCCCGGAGACCGAAGGGCCGCGGGTCATCTTCATCGT
CGGCGTCAACGGTGTCGGTAAGACGACGACTATCGGCAAACTGGCCAAGTATTACAAGCAGCAGGGGAAATCGGTCATGC
TCGCCGCCGGCGATACCTTCCGTGCCGCTGCCTCGGAACAGCTGACCATCTGGGCTGACCGCGTCGGCGTGCCCATCGTC
AAGCACCAGGAAGGGGCGGACGCAGCGGCTGTCGTCTTCGATGCGACGGCGTCGGCCAAGGCCAAGGGCATGGATATCCT
CCTTGTCGATACGGCCGGCCGCCTGCAGACCAAGTCCAACCTCATGGAAGAATTGCGGAAAATGGCCCGCGTCGCCGGCC
GCAACATCGAAGGGGCGCCGCAGGAAACGCTCCTCGTCCTCGATGCCACGACGGGGCAGAACGCTGTCAGCCAGGCCAAG
CTCTTCGGCGACGTCGTTCCCTTGACGGGCGTCGTCCTGACCAAGCTCGACGGAACAGCCAAAGGCGGCGTCATCTTGTC
GGTGAAACGGGAATTAGGCGTCCCTGTCCGCTGGGTCGGCGTCGGCGAAGGCGTCGACGACCTGCGCCCCTTCGATGCAG
CTCAGTTCGCCGATGCTCTGTTCGATAAAAACGTCGCCGCCCGCGAAGCCAGCGACGAAGATTAG

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(21-80)

SRP54-type protein, GTPase domain (SRP54)

(101-301)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G0VLY9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

51.645

96.815

0.5

Multiple sequence alignment