Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   WS74_RS05690 Genome accession   NZ_CP009223
Coordinates   1140865..1142241 (-) Length   458 a.a.
NCBI ID   WP_009496193.1    Uniprot ID   A0A075U777
Organism   Weissella ceti strain WS74     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1135865..1147241
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WS74_RS05680 (WS74_1077) secA 1136493..1138859 (-) 2367 WP_009496191.1 preprotein translocase subunit SecA -
  WS74_RS05685 (WS74_1078) gltX 1139306..1140799 (-) 1494 WP_009496192.1 glutamate--tRNA ligase -
  WS74_RS05690 (WS74_1079) radA 1140865..1142241 (-) 1377 WP_009496193.1 DNA repair protein RadA Machinery gene
  WS74_RS05695 (WS74_1080) - 1142257..1142799 (-) 543 WP_009496194.1 deoxyuridine 5'-triphosphate nucleotidohydrolase -
  WS74_RS05700 (WS74_1081) - 1142892..1143170 (-) 279 WP_009496195.1 GNAT family N-acetyltransferase -
  WS74_RS05705 (WS74_1082) - 1143172..1143726 (-) 555 WP_009496196.1 NlpC/P60 family protein -
  WS74_RS07400 (WS74_1083) - 1143804..1144574 (-) 771 WP_051747850.1 CHAP domain-containing protein -
  WS74_RS05715 (WS74_1084) - 1144895..1147048 (-) 2154 WP_009496198.1 ATP-dependent Clp protease ATP-binding subunit -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49514.44 Da        Isoelectric Point: 4.9824

>NTDB_id=127323 WS74_RS05690 WP_009496193.1 1140865..1142241(-) (radA) [Weissella ceti strain WS74]
MAKVKTQFACSNCGAISQRYMGRCPSCGAWGTLVEEVQQPEKADRKSRVNLAGEVAKFEKLDDINIEETLRVTSNSNEFD
RVLGGGIVPGSLVLIGGDPGIGKSTLLLQVSGSLANSGQKVLYVSGEESASQIKLRAERLGVQGDADFYLYPETDMSQIR
KAIDELKPDLVVIDSIQTMQEPDVQSSVGSVAQIRETTAELLQIAKTNGVTIFIVGHVTKEGNIAGPKILEHMVDTVLYF
EGDNQRSFRLLRAVKNRFGSTNELGVFEMEHEGLAEVANPSQMFLEERLEDASGSAVVVALEGTRPVLVELQALVTPTVF
GNAQRTSAGIDRNRVSVLMAVLEKRANLLLQNQDAYVRAAGGVRLDEPAIDLAIAMAIASSYRDKGTRPGDAFVGEIGLT
GEIRRVPRLHDRIAEAQTLGFKRVFVPYSGLDAKNKVEGIEIIGVKTLSDALRLGLDG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=127323 WS74_RS05690 WP_009496193.1 1140865..1142241(-) (radA) [Weissella ceti strain WS74]
ATGGCAAAAGTAAAAACACAATTTGCGTGTTCTAACTGTGGTGCGATTTCACAACGCTATATGGGACGTTGTCCCAGTTG
TGGCGCATGGGGAACACTTGTTGAAGAAGTCCAACAACCTGAAAAAGCAGACCGTAAGTCACGTGTTAACCTAGCTGGTG
AAGTGGCGAAGTTTGAAAAGTTGGATGACATTAATATAGAAGAAACTTTGCGTGTGACCTCAAATTCAAATGAATTTGAC
CGCGTATTAGGGGGTGGCATCGTTCCAGGATCACTTGTCTTAATTGGTGGTGATCCCGGAATTGGAAAATCTACACTACT
CTTACAAGTTTCTGGTTCATTAGCTAATTCAGGCCAAAAAGTGCTGTATGTTTCTGGTGAAGAAAGTGCATCACAAATCA
AGTTGCGAGCTGAACGTCTTGGCGTGCAAGGCGATGCGGATTTCTACTTATATCCGGAAACAGATATGAGCCAAATTCGA
AAAGCGATTGATGAATTGAAGCCTGACTTAGTTGTGATTGATTCAATTCAAACGATGCAAGAACCGGATGTGCAATCAAG
TGTTGGGTCAGTTGCACAAATTCGTGAAACGACCGCCGAACTCTTACAAATTGCGAAAACCAATGGCGTTACAATCTTCA
TTGTCGGCCATGTTACTAAGGAAGGTAACATTGCAGGACCAAAGATTTTGGAACACATGGTGGACACCGTTTTGTATTTT
GAAGGTGATAATCAACGTAGCTTCCGATTGTTGCGTGCGGTTAAGAATCGTTTTGGATCAACCAATGAATTGGGTGTTTT
TGAAATGGAACATGAAGGACTAGCTGAAGTAGCGAATCCTTCTCAAATGTTCTTAGAAGAACGGCTAGAAGATGCGAGTG
GGTCGGCCGTTGTTGTTGCGTTAGAAGGGACACGACCAGTGCTAGTTGAACTACAAGCCTTGGTAACGCCTACTGTCTTT
GGTAATGCGCAGCGTACCTCAGCTGGAATTGATCGTAATCGTGTGTCCGTTTTGATGGCTGTCTTAGAGAAACGCGCCAA
TCTATTACTGCAAAATCAAGATGCCTATGTGCGAGCTGCTGGTGGTGTTCGCTTAGATGAACCTGCGATTGACCTTGCGA
TTGCAATGGCAATTGCATCTAGTTATCGCGATAAAGGAACACGACCGGGTGATGCTTTTGTGGGTGAAATTGGTCTAACA
GGTGAAATCAGGCGTGTTCCACGTTTGCATGATCGTATTGCGGAAGCGCAAACGCTTGGCTTCAAGCGAGTGTTCGTACC
GTACTCAGGTTTGGACGCTAAAAACAAGGTCGAAGGCATTGAAATCATTGGGGTTAAAACATTATCTGATGCGTTACGCT
TAGGACTTGATGGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(366-428)

AAA domain (AAA_25)

(82-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A075U777

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

61.589

98.908

0.609
  radA Streptococcus pneumoniae Rx1

61.589

98.908

0.609
  radA Streptococcus pneumoniae D39

61.589

98.908

0.609
  radA Streptococcus pneumoniae R6

61.589

98.908

0.609
  radA Streptococcus pneumoniae TIGR4

61.589

98.908

0.609
  radA Streptococcus mitis SK321

61.589

98.908

0.609
  radA Bacillus subtilis subsp. subtilis str. 168

59.041

100

0.592

Multiple sequence alignment