Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   ID12_RS09990 Genome accession   NZ_CP009108
Coordinates   1989914..1993618 (+) Length   1234 a.a.
NCBI ID   WP_050827123.1    Uniprot ID   -
Organism   Bacillus altitudinis strain GR-8     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1984914..1998618
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ID12_RS09975 (ID12_10365) - 1985212..1985622 (+) 411 WP_008348626.1 DoxX family protein -
  ID12_RS09980 (ID12_10370) - 1985814..1986260 (-) 447 WP_007499129.1 ferritin-like domain-containing protein -
  ID12_RS09985 (ID12_10375) addB 1986427..1989936 (+) 3510 WP_050827122.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  ID12_RS09990 (ID12_10380) addA 1989914..1993618 (+) 3705 WP_050827123.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  ID12_RS09995 (ID12_10385) sbcD 1993644..1994819 (+) 1176 WP_007499123.1 exonuclease subunit SbcD -
  ID12_RS10000 (ID12_10390) sbcC 1994816..1998211 (+) 3396 WP_050827124.1 exonuclease subunit SbcC -
  ID12_RS10005 (ID12_10395) - 1998229..1998528 (+) 300 WP_017360217.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 142672.58 Da        Isoelectric Point: 5.1229

>NTDB_id=126860 ID12_RS09990 WP_050827123.1 1989914..1993618(+) (addA) [Bacillus altitudinis strain GR-8]
MQIPKPNNSTWTDDQWNAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEQPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELSKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPAPEQWMRAFLSLYDVDTQTKVEELPFYPYIKEDLSLVLRSCQELLEQA
LTLSKEPGGPAPRAENFIDDLEQVNELIRHQDDFGTLYELLPNINFKRLKTCKGDDYDPALLEKATDARNQAKKQLEKLK
DEYFMRSPDQHLKSLAEMKPVVETLVELVIQYGERFERAKQEKSIVDFSDLEHYCLRILAKQDAEGNLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGKHPAEGNRFMVGDVKQSIYRFRLAEPMLFLNKYKQFKADSKETGKRIDLNKNFR
SRADVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPQSEDTTSEMLLVHLDQHDTENDEEREELETVQFEARVIAKK
IKELVEQPFQVYDAKQQLTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPLVHLDENEMALIRTSDKKGTYYDAVKAFMSVTHSDHPTCRKLERFFLQLRKWRDFSIQHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLIAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAANGDDWLLPDFERYQARTYLDFIGPALIRHQAMSSLLEESREVVLSHPSSFSITFHQASDL
LQEDISLEKQKQDEVVQALMEGVPVQGYGDYEEEVKERLSWRYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSVRKAR
ATLYERPAFMKKKTLTAAEQGTAMHTVMQHIPLPSNEPYDESRVEELLHSLKEKDLLTDEQIQSINREGIVAFFSTAIGQ
KLRKADWVKREVSFSMVLPVKEVYSHIDAEGEPVLIQGMIDCLFEADGKLYLLDYKTDRVTGRFSGGLEEAEAMLKKRYE
TQISLYTAAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=126860 ID12_RS09990 WP_050827123.1 1989914..1993618(+) (addA) [Bacillus altitudinis strain GR-8]
ATGCAAATACCAAAACCGAATAACAGTACTTGGACAGATGACCAATGGAACGCCATCGTTTCAGAAGGACAAGATATTCT
TGTTGCTGCGGCGGCAGGGTCAGGGAAAACAGCGGTCTTGGTTGAACGGCTCATTCGAAAAATGACTCGGCCGGAGCAGC
CAGTTGATGTGGACAGGCTGCTCGTAGTGACTTTTACCAATGCTTCAGCAGCAGAAATGAAGCACCGGATCACTGAAGCA
CTTGAAAAAGAACTATCTAAAAACCCTGGTTCCCTTCATATGCGGAGACAGCTGTCACTTATGAATCGTGCCAATATTTC
TACCTTGCACTCTTTTTGTTTGCAAGTTCTCCGTACCTTTTATTATGAGATTGACCTTGATCCAGGTTTTCGTTTGGCTG
ATCAAACAGAAGGAGAATTATTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGATGAGTATAAGGCTGGAAAGCCGTCA
TTCTTTGAACTCGTTGATCGTTACACATCTGATCGGCATGATTTAGATCTTCAATGGCTTGTCAAAAGAATTTATGAGTT
TTCTAGGTCACATCCAGCGCCCGAACAATGGATGCGGGCATTCCTTTCTCTTTATGATGTGGATACACAGACAAAGGTAG
AAGAGTTACCCTTTTATCCATATATCAAAGAGGACCTTTCTCTCGTTTTAAGAAGCTGTCAGGAACTGCTGGAACAAGCG
CTCACTTTATCAAAAGAGCCGGGCGGTCCAGCACCGAGAGCAGAGAATTTTATAGATGATTTAGAGCAAGTAAATGAATT
AATAAGGCATCAAGATGATTTTGGAACACTTTATGAGCTTTTGCCAAACATCAATTTTAAAAGGTTAAAGACTTGCAAAG
GGGATGACTATGACCCAGCTCTGTTAGAAAAAGCAACAGATGCACGCAATCAAGCCAAAAAACAATTAGAAAAGCTAAAA
GATGAATACTTTATGCGCAGTCCCGATCAGCATTTAAAAAGCTTGGCTGAAATGAAGCCAGTTGTAGAAACACTTGTAGA
GCTTGTGATTCAATATGGCGAGCGTTTCGAAAGAGCAAAACAAGAAAAATCAATCGTAGACTTTTCGGATCTAGAACATT
ATTGTTTGCGCATTTTAGCGAAGCAGGACGCAGAAGGAAATCTCATTGAAACAGAAGCGGCTAAATACTATCAACAGCAA
TTTGAAGAAGTACTCGTGGATGAATACCAGGATACAAACCTTGTCCAAGAAACGATTTTAAAGCTCGTGTCTAAAGGAAA
ACATCCTGCCGAAGGCAACCGCTTTATGGTTGGTGATGTGAAGCAGTCCATTTATCGCTTTAGACTTGCAGAGCCGATGC
TCTTTTTAAATAAATATAAACAATTCAAAGCAGATAGCAAAGAAACAGGTAAAAGAATTGATCTAAATAAAAACTTCCGC
AGCCGGGCTGATGTTTTAGACAGTACCAACTTTCTGTTCAAACAGCTGATGGGAGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCGCAAAGCGAAGACACGACGTCAGAAATGCTTCTTGTTCATTTAG
ATCAGCACGACACAGAAAACGATGAAGAACGAGAAGAGTTAGAGACGGTACAATTTGAGGCGAGAGTGATTGCGAAGAAA
ATAAAAGAACTGGTAGAACAGCCTTTTCAAGTGTACGATGCAAAACAGCAGCTGACCCGCAACTTGCAGTATCGAGACAT
TGTGATCTTGCTTCGCTCAATGCCGTGGGCTCCGCAAATGATGGAAGAGTTGAAGAAGCAGGGAATCCCTGTTTATGCGA
ATCTTTCCTCAGGTTATTTTGAAGCTACAGAAGTATCTGTCATTCTTTCTTTGTTAAAAGTGATTGATAATCCATATCAA
GATATTCCGCTCGCTGCTGTTTTAAGGTCACCTCTTGTTCATTTAGACGAAAATGAGATGGCACTGATTAGAACAAGTGA
TAAAAAAGGCACTTATTATGACGCTGTGAAGGCATTTATGAGTGTGACACACTCTGATCATCCGACATGCAGAAAGCTGG
AGCGTTTCTTCCTTCAATTACGCAAATGGCGTGATTTTTCGATTCAGCATTCGGTAGCAGAACTGATTTGGGAAGTGTAC
CGAGATACACAATATCTAGATTATGTCGGCGGGATGCCAGGAGGTAAGCAAAGACAGGCAAACCTTCGGGCTTTATATGA
TCGAGCGAAACAGTATGAAAAGGCTGCATTTAGAGGGCTGTTTCGATTTCTTCGATTTATTGAAAGAATGCAAGAGCGTG
GAGATGATCTTGGAGCGGCAAAAACGTTCAGTGAAACAGAGGATGTTGTCCGCATGATGACCATTCATAGCAGTAAAGGA
CTCGAATTCCCTGTTGTGTTTACTGCAGGACTTGGCAGGAACTTTAACATGATGGATTTAAACCAGTCCTATTTGCTCGA
TAAAGAATTAGGTTTTGGCAGCAAATATATTCATCCTGAGCTGAGAATTAGTTATGCAACATTACCGCTGATCGCGATGA
AAAAAAAGATGAGAAAAGAACTATTATCTGAAGAACTGAGGGTTCTATATGTGGCGCTCACAAGGGCAAAGGAAAAATTA
TTTTTAGTCGGCTCTGTCAAAAATCAAGTGAAAGCTTTAAGCAAGTGGCAAAATGCCGCAAACGGAGACGACTGGCTTCT
TCCTGATTTTGAACGATATCAAGCGAGAACATACTTAGATTTTATTGGCCCAGCTCTTATCCGTCATCAAGCGATGTCTT
CTCTTTTAGAAGAATCGAGAGAAGTCGTTCTCTCGCATCCATCATCATTTTCTATCACCTTTCATCAAGCGTCCGATCTT
CTACAAGAAGACATATCACTTGAAAAACAAAAACAAGACGAAGTGGTACAAGCATTAATGGAAGGTGTGCCTGTTCAAGG
ATATGGCGATTACGAGGAGGAAGTAAAAGAAAGACTTTCATGGCGTTATCCATATTTAGCGGCCTCCCAAGTAGGGACAA
AGCAGTCAGTTTCTGAAATCAAAAGAATGAAAGAAATACAGGATGAATACAGTGTGCCTTCTTCCGTTCGAAAAGCTCGT
GCGACATTGTACGAACGACCGGCTTTTATGAAGAAAAAAACACTCACAGCCGCAGAACAAGGGACGGCCATGCATACAGT
CATGCAGCATATCCCTCTTCCTTCAAATGAACCATATGATGAATCTCGTGTAGAAGAGCTGCTCCATTCTTTAAAAGAGA
AAGACTTACTAACAGATGAACAAATCCAGTCCATCAATCGAGAAGGAATTGTGGCCTTCTTTTCTACTGCTATTGGTCAA
AAACTGCGGAAAGCGGACTGGGTGAAACGAGAAGTATCGTTTAGTATGGTTTTACCGGTTAAAGAGGTATACAGCCATAT
TGATGCAGAAGGTGAACCTGTGCTCATTCAAGGAATGATTGATTGTTTATTTGAAGCAGATGGCAAGCTCTATTTACTTG
ATTATAAAACAGATCGAGTGACTGGCAGATTCTCAGGTGGCTTAGAGGAAGCTGAAGCGATGTTAAAAAAACGCTATGAA
ACGCAAATATCGTTGTATACAGCAGCGGTAGAGCGTTTAACAAACAGAACATTAGAGGAGAAAATCCTTTATTTCTTTGA
TGGAAATCTAGAAATTTCTTTATAG

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-463)

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

66.855

100

0.67

Multiple sequence alignment