Detailed information NTG0126735

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: WV34_RS01505
• Genome accession: NZ_CP011252
• Coordinates: 270811..271932 (+)
• Length: 373 a.a.
• NCBI ID: WP_013350919.1
• Uniprot ID: A0A9P1NGF1
• Organism: Bacillus amyloliquefaciens strain MT45
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 265811..276932

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WV34_RS01485 (WV34_01455)	-	266134..267129 (-)	996	WP_088612105.1	DUF5105 domain-containing protein	-
WV34_RS01490 (WV34_01460)	-	267247..268642 (+)	1396	Protein_255	YcdB/YcdC domain-containing protein	-
WV34_RS01495 (WV34_01465)	-	268843..270201 (+)	1359	WP_088612106.1	YcdB/YcdC domain-containing protein	-
WV34_RS01500 (WV34_01470)	-	270198..270701 (-)	504	WP_088612107.1	M15 family metallopeptidase	-
WV34_RS01505 (WV34_01475)	rapF	270811..271932 (+)	1122	WP_013350919.1	tetratricopeptide repeat protein	Regulator
WV34_RS01510 (WV34_01480)	-	272046..272825 (+)	780	WP_088612108.1	glucose 1-dehydrogenase	-
WV34_RS01515 (WV34_01485)	-	272909..274588 (+)	1680	WP_088612109.1	alpha-glucosidase	-
WV34_RS01520 (WV34_01490)	-	274573..275067 (-)	495	WP_088612110.1	DUF4188 domain-containing protein	-
WV34_RS01525 (WV34_01495)	-	275068..275613 (-)	546	WP_088612111.1	PadR family transcriptional regulator	-
WV34_RS01530 (WV34_01500)	-	275822..276781 (+)	960	WP_045508664.1	metal ABC transporter substrate-binding protein	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44347.46 Da
• Isoelectric Point: 5.0035

>NTDB_id=126735 WV34_RS01505 WP_013350919.1 270811..271932(+) (rapF) [Bacillus amyloliquefaciens strain MT45]
MKSKIPSEEVAVKMNEWYKLIRAFEADKAEALKREIQYELEDMEENQDLLLYFSLMEFRHRIMLDKLKPMKDGDCQPPFS
EMLTEIEMNQQKLTGLLEYYFYYFRGMYEFKQKNFISAISHYKRAEEKLEYVEDDIEKAEFLYKISEVYYHIKQTFFSMN
YAGQALDIYKKYELYGMRRVQCKFIIAGNLMDAQQHEKALEYLECALEHAERLDDHYMIAAAYYNIGHCMYSLGDFCKAA
EDFERAAAGFEAHSFQQVIQAVFSLTHIYFKTERYSDAAASYARGMKSAAEWEDEIYMTKFRLIKELYQGDGGQDSLKEY
FDILESKELLADTEELLQDAAHFFHKRENYKAASYFFSRLVGVQKRLQMNNYA

Nucleotide

• Length: 1122 bp

>NTDB_id=126735 WV34_RS01505 WP_013350919.1 270811..271932(+) (rapF) [Bacillus amyloliquefaciens strain MT45]
ATGAAATCCAAGATTCCATCTGAAGAAGTAGCCGTAAAGATGAATGAATGGTATAAGCTGATCCGCGCGTTTGAAGCCGA
TAAAGCTGAAGCGCTGAAGCGGGAGATTCAATATGAACTCGAAGATATGGAAGAAAATCAGGATTTATTATTGTATTTTT
CATTGATGGAATTTAGGCATCGCATCATGCTGGATAAATTAAAACCCATGAAAGACGGCGACTGCCAGCCTCCGTTCTCT
GAAATGCTCACTGAGATTGAGATGAACCAGCAAAAGCTGACCGGCTTACTGGAATATTATTTTTACTATTTCAGGGGAAT
GTATGAATTTAAGCAGAAAAACTTCATCTCAGCGATTTCTCATTATAAACGGGCTGAGGAAAAACTAGAATATGTGGAAG
ATGACATAGAGAAAGCCGAGTTTCTGTATAAAATTTCTGAAGTGTACTATCATATCAAGCAGACTTTCTTTTCGATGAAT
TACGCGGGACAGGCGCTTGATATTTACAAGAAATATGAATTATACGGAATGAGAAGGGTGCAGTGCAAATTTATCATTGC
CGGTAATCTGATGGACGCCCAGCAGCATGAAAAAGCGCTGGAATACCTTGAATGCGCCTTGGAGCACGCGGAGCGGCTTG
ATGATCACTATATGATTGCTGCGGCCTATTATAATATCGGGCACTGTATGTACAGTTTGGGTGACTTTTGCAAGGCGGCG
GAAGACTTTGAACGAGCGGCTGCCGGTTTTGAAGCGCACAGCTTCCAGCAGGTCATCCAGGCTGTTTTTTCACTCACGCA
TATCTACTTTAAAACGGAAAGATACAGTGACGCCGCCGCCAGCTACGCGCGGGGCATGAAAAGCGCGGCGGAGTGGGAAG
ACGAGATCTATATGACAAAGTTCCGTCTGATTAAGGAGCTTTATCAGGGTGACGGCGGACAGGATTCGCTCAAAGAGTAT
TTTGACATTCTGGAATCAAAGGAGCTGTTGGCCGACACTGAGGAACTGCTTCAGGACGCGGCTCATTTCTTTCATAAACG
GGAGAACTACAAAGCCGCATCTTATTTTTTCAGCAGGCTTGTCGGCGTGCAAAAAAGGCTGCAAATGAACAATTACGCAT
AA

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (187-251)

3D structure

• AlphaFold DB: A0A9P1NGF1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.588	99.464	0.424
rapC	Bacillus subtilis subsp. subtilis str. 168	40.323	99.732	0.402