Detailed information NTG0126439

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: NCDO2118_RS09740
• Genome accession: NZ_CP009054
• Coordinates: 2017622..2018269 (-)
• Length: 215 a.a.
• NCBI ID: WP_012898342.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis NCDO 2118
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2012622..2023269

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NCDO2118_RS09715 (NCDO2118_1875)	atpF	2012691..2013197 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
NCDO2118_RS09720 (NCDO2118_1876)	atpB	2013212..2013925 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
NCDO2118_RS09725 (NCDO2118_1877)	-	2013970..2014185 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
NCDO2118_RS09730 (NCDO2118_1878)	-	2014370..2015146 (-)	777	WP_012898340.1	alpha/beta hydrolase	-
NCDO2118_RS09735 (NCDO2118_1879)	comEC	2015431..2017641 (-)	2211	WP_012898341.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
NCDO2118_RS09740 (NCDO2118_1880)	comEA	2017622..2018269 (-)	648	WP_012898342.1	ComEA family DNA-binding protein	Machinery gene
NCDO2118_RS09745 (NCDO2118_1881)	-	2018329..2019690 (-)	1362	WP_012898343.1	ABC transporter permease	-
NCDO2118_RS09750 (NCDO2118_1882)	-	2019687..2020619 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
NCDO2118_RS09755 (NCDO2118_1883)	-	2020724..2021122 (-)	399	WP_004255236.1	hypothetical protein	-
NCDO2118_RS09760 (NCDO2118_1884)	-	2021231..2021794 (-)	564	WP_012898344.1	GNAT family N-acetyltransferase	-
NCDO2118_RS09765 (NCDO2118_1885)	-	2021967..2023145 (-)	1179	WP_012898345.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23619.79 Da
• Isoelectric Point: 4.7531

>NTDB_id=126439 NCDO2118_RS09740 WP_012898342.1 2017622..2018269(-) (comEA) [Lactococcus lactis subsp. lactis NCDO 2118]
MDKILEKVKEYWKMIVLIVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISPDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
VTELQKLTGVGQKKAQDIIDFRTKNGDFISLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=126439 NCDO2118_RS09740 WP_012898342.1 2017622..2018269(-) (comEA) [Lactococcus lactis subsp. lactis NCDO 2118]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAATTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGCCAGATGAACGTCTAGTTGATCTAATTAAGGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
GTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATTGATTTTCGGACGAAAAATGGTGA
TTTCATATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterProScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	74.312	100	0.753
comEA/celA/cilE	Streptococcus mitis SK321	43.981	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	42.661	100	0.433
comEA/celA/cilE	Streptococcus mitis NCTC 12261	40.826	100	0.414
comEA/celA/cilE	Streptococcus pneumoniae Rx1	40.639	100	0.414
comEA/celA/cilE	Streptococcus pneumoniae D39	40.639	100	0.414
comEA/celA/cilE	Streptococcus pneumoniae R6	40.639	100	0.414
comEA	Latilactobacillus sakei subsp. sakei 23K	35.683	100	0.377