Detailed information NTG0126289

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: IR04_RS18330
• Genome accession: NZ_CP009042
• Coordinates: 2889191..2890207 (-)
• Length: 338 a.a.
• NCBI ID: WP_000520444.1
• Uniprot ID: A0A0F0B833
• Organism: Vibrio cholerae O1 biovar El Tor strain FJ147
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2884191..2895207

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IR04_RS18305 (IR04_18225)	aroK	2885074..2885598 (-)	525	WP_000818616.1	shikimate kinase AroK	-
IR04_RS18310 (IR04_18230)	pilQ	2885785..2887500 (-)	1716	WP_000788426.1	type IV pilus secretin PilQ family protein	Machinery gene
IR04_RS18315 (IR04_18235)	pilP	2887526..2888044 (-)	519	WP_000792032.1	pilus assembly protein PilP	Machinery gene
IR04_RS18320 (IR04_18240)	pilO	2888034..2888624 (-)	591	WP_000157690.1	type 4a pilus biogenesis protein PilO	Machinery gene
IR04_RS18325 (IR04_18245)	pilN	2888617..2889201 (-)	585	WP_000902745.1	PilN domain-containing protein	Machinery gene
IR04_RS18330 (IR04_18250)	pilM	2889191..2890207 (-)	1017	WP_000520444.1	type IV pilus assembly protein PilM	Machinery gene
IR04_RS18335 (IR04_18255)	-	2890350..2892827 (+)	2478	WP_000668733.1	penicillin-binding protein 1A	-
IR04_RS18340 (IR04_18260)	oxyR	2892901..2893794 (-)	894	WP_001025909.1	DNA-binding transcriptional regulator OxyR	-
IR04_RS18345 (IR04_18265)	-	2893934..2894665 (+)	732	WP_000495223.1	glutathione peroxidase	-

Sequence

Protein

• Length: 338 a.a.
• Molecular weight: 37707.44 Da
• Isoelectric Point: 7.3328

>NTDB_id=126289 IR04_RS18330 WP_000520444.1 2889191..2890207(-) (pilM) [Vibrio cholerae O1 biovar El Tor strain FJ147]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide

• Length: 1017 bp

>NTDB_id=126289 IR04_RS18330 WP_000520444.1 2889191..2890207(-) (pilM) [Vibrio cholerae O1 biovar El Tor strain FJ147]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTTTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCA
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACGCAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
GTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGCAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-221)

• Type IV pilus assembly protein PilM; (PilM_2) (241-328)

3D structure

• AlphaFold DB: A0A0F0B833

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio cholerae strain A1552	100	100	1
pilM	Vibrio campbellii strain DS40M4	48.521	100	0.485