Detailed information NTG0125595

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: FORC12_RS14045
• Genome accession: NZ_CP010998
• Coordinates: 2774777..2775859 (+)
• Length: 360 a.a.
• NCBI ID: WP_001548033.1
• Uniprot ID: A0A0H2XJ41
• Organism: Staphylococcus aureus strain FORC_012 isolate human sputum
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2769777..2780859

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FORC12_RS14025 (FORC12_2543)	-	2770154..2771224 (-)	1071	WP_000460983.1	GGDEF domain-containing protein	-
FORC12_RS14030 (FORC12_2544)	-	2771542..2772597 (+)	1056	WP_000258150.1	MraY family glycosyltransferase	-
FORC12_RS14035 (FORC12_2545)	-	2772762..2773403 (-)	642	WP_000192949.1	YigZ family protein	-
FORC12_RS14040 (FORC12_2546)	fakB1	2773547..2774413 (+)	867	WP_000686343.1	fatty acid kinase binding subunit FakB1	-
FORC12_RS14045 (FORC12_2547)	comFA	2774777..2775859 (+)	1083	WP_001548033.1	DEAD/DEAH box helicase	Machinery gene
FORC12_RS14050 (FORC12_2548)	-	2775852..2776526 (+)	675	WP_001057965.1	ComF family protein	-
FORC12_RS14055 (FORC12_2549)	raiA	2776587..2777159 (+)	573	WP_000617735.1	ribosome-associated translation inhibitor RaiA	-
FORC12_RS14060 (FORC12_2550)	secA	2777573..2780104 (+)	2532	WP_000506531.1	preprotein translocase subunit SecA	-
FORC12_RS14065	-	2780146..2780250 (+)	105	WP_001790092.1	hypothetical protein	-

Sequence

Protein

• Length: 360 a.a.
• Molecular weight: 42111.65 Da
• Isoelectric Point: 9.8307

>NTDB_id=125595 FORC12_RS14045 WP_001548033.1 2774777..2775859(+) (comFA) [Staphylococcus aureus strain FORC_012 isolate human sputum]
MDNVTRYKITESSQSSSQAYYHLSFELSEQQSYASEHIVRAIRKRQTILLYAVTGAGKTEMMFQGIQYARIQGDNIAIVS
PRVDVVVEISKRIKDAFLNEDIDILHQQSRQQFEGHFVVCTVHQLYRFKQHFDTIFIDEVDAFPLSMDKNLQQALKSSSK
VEHATIYMTATPPKQLLSEIPHENIIKLPARFHKKSLPVPKYRYFKLNNKKIQKMLYRILQDQINNQRYTLVFFNNIETM
IKTFSVYKQKITKLTYVHSEDVFRFEKVEQLRNGHFDVIFTTTILERGFTMANLDVVVIDAHQYTQEALIQIAGRVGRKL
ECPTGKVLFFHEGVSMNMIQAKKEIQRMNKLALKRGWIDE

Nucleotide

• Length: 1083 bp

>NTDB_id=125595 FORC12_RS14045 WP_001548033.1 2774777..2775859(+) (comFA) [Staphylococcus aureus strain FORC_012 isolate human sputum]
ATGGATAATGTAACAAGATATAAAATAACAGAGAGTTCGCAAAGTTCATCACAAGCATATTATCATCTCTCATTTGAATT
GTCGGAACAGCAGTCTTATGCCTCAGAACATATTGTTCGAGCCATTAGAAAGAGACAAACGATTTTGTTATATGCCGTAA
CAGGTGCAGGTAAGACAGAAATGATGTTTCAAGGCATTCAATATGCAAGAATACAGGGAGATAATATAGCTATTGTGTCA
CCACGTGTAGATGTTGTTGTAGAAATTAGTAAACGTATTAAAGACGCATTTCTTAATGAAGATATAGACATACTACACCA
GCAATCAAGACAACAATTTGAAGGGCATTTTGTTGTATGCACAGTGCATCAACTTTACCGATTCAAACAGCACTTTGATA
CTATTTTTATTGATGAAGTCGATGCCTTTCCTTTATCAATGGATAAAAATCTACAACAAGCATTGAAGTCATCTTCTAAA
GTTGAACATGCAACAATTTATATGACAGCAACACCACCGAAACAACTTCTGTCAGAGATTCCCCACGAAAATATAATTAA
ATTGCCAGCTCGCTTTCATAAAAAATCACTTCCAGTTCCTAAATATCGTTATTTCAAACTTAATAATAAGAAGATTCAGA
AAATGTTATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATG
ATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGTTTTTCGCTTTGAAAA
AGTTGAACAATTAAGGAATGGACATTTCGATGTCATTTTTACTACGACAATATTAGAACGTGGATTTACAATGGCAAATT
TGGATGTTGTTGTTATCGATGCACATCAATATACTCAAGAGGCTTTAATACAAATTGCTGGACGTGTTGGACGAAAATTA
GAATGTCCTACTGGAAAAGTATTGTTTTTCCATGAAGGAGTAAGTATGAATATGATTCAAGCTAAAAAAGAGATTCAAAG
GATGAACAAATTAGCATTAAAAAGAGGTTGGATTGATGAATAA

Domains

Predicted by InterProScan.

• Helicase conserved C-terminal domain (Helicase_C) (215-318)

• Type III restriction enzyme, res subunit (ResIII) (25-173)

3D structure

• AlphaFold DB: A0A0H2XJ41

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	38.938	94.167	0.367