Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   B723_RS07430 Genome accession   NZ_CP010945
Coordinates   1606444..1608663 (-) Length   739 a.a.
NCBI ID   WP_017336115.1    Uniprot ID   -
Organism   Pseudomonas fluorescens NCIMB 11764     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1601444..1613663
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B723_RS07410 (B723_07430) hslU 1601529..1602866 (-) 1338 WP_017336112.1 ATP-dependent protease ATPase subunit HslU -
  B723_RS07415 (B723_07435) hslV 1602922..1603452 (-) 531 WP_007899242.1 ATP-dependent protease subunit HslV -
  B723_RS07420 (B723_07440) - 1603741..1604439 (-) 699 WP_017336113.1 SPOR domain-containing protein -
  B723_RS07425 (B723_07445) argS 1604441..1606177 (-) 1737 WP_031318290.1 arginine--tRNA ligase -
  B723_RS07430 (B723_07450) priA 1606444..1608663 (-) 2220 WP_017336115.1 primosomal protein N' Machinery gene
  B723_RS07435 (B723_07455) rpmE 1608834..1609064 (+) 231 WP_017336116.1 50S ribosomal protein L31 -
  B723_RS07440 (B723_07460) - 1609079..1609879 (+) 801 WP_031318292.1 thermonuclease family protein -
  B723_RS07445 (B723_07465) - 1610062..1611330 (+) 1269 WP_017336118.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 81942.44 Da        Isoelectric Point: 8.4232

>NTDB_id=125448 B723_RS07430 WP_017336115.1 1606444..1608663(-) (priA) [Pseudomonas fluorescens NCIMB 11764]
MPDAILRLALPSPLRRLFDYRAPAGVLRDQLQPGMRLRVPFGRREMIGILVEVTDTSEVPAEKLKPALALLDATPPLPPA
LFKLCLWTSQYYQHSLGDTLSWALPVLLRQGELAEARQERFWSAAPGASLDDPRIARAPRQREALATLAQHPHGVAHQLL
SKLMLSKDSLDLLLAKDLVQVEIRKHAPGARHEHWLAQPELPLNPEQRAAYEAIRAGFDSYHAFLLAGVTGSGKTEVYLQ
LIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALIHSAVNDRERLEAWLAARDGDADIIIGTRSALFTPMKNPGL
IIIDEEHDGSYKQQEGLRYHARDLALVRARQENIPIVLGSATPSLESLHNAYTGRYGLLRLNERAGGAKQPRFLRLDVKS
RPLDSGISGPMQQAIGQTLAAGQQVLVFLNRRGFAPTLLCHDCGWMSECQRCDARMTVHQRSGELRCHHCGYVERVPRHC
PKCGKVDLRPVGAGTERAEERLAILFPDVPVLRVDRDSTSRKDAMNQLFATIQKGQPCILVGTQMLAKGHHFPRVTLVSI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVIIQTHLADHPLLVQLTEQGYFAFAEQALSERRSAGLPPFAHL
ALLRAEAHKPGQAEGFLDEACSEAERLLAEQNLTGIELLGPVPAPMERRAGRYRAQLLLQATARAPLHRLLANWLLVLEQ
MPSGRAVRWSLDVDPVDLY

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=125448 B723_RS07430 WP_017336115.1 1606444..1608663(-) (priA) [Pseudomonas fluorescens NCIMB 11764]
GTGCCCGACGCCATTCTGCGCCTCGCCCTGCCTTCGCCCCTGCGCCGCCTGTTCGATTACCGCGCCCCTGCCGGGGTCCT
GCGCGACCAGTTGCAACCGGGCATGCGCTTGCGGGTGCCGTTCGGCCGGCGGGAGATGATCGGGATTCTGGTGGAGGTCA
CCGACACCAGTGAAGTACCGGCAGAAAAACTCAAACCGGCCCTGGCCCTGCTCGACGCCACACCGCCCTTGCCACCCGCA
TTATTCAAGCTGTGCCTGTGGACCTCTCAGTATTATCAGCACAGCCTCGGCGACACCTTGAGCTGGGCGCTGCCGGTGTT
GTTGCGTCAGGGTGAGCTGGCCGAAGCGCGCCAGGAACGTTTCTGGTCCGCCGCGCCTGGCGCCAGCCTCGATGACCCGC
GTATCGCCCGCGCCCCGCGTCAGCGTGAAGCGCTGGCGACACTGGCCCAGCATCCCCACGGCGTCGCTCATCAATTGCTG
AGCAAACTGATGCTGAGCAAGGACAGCCTCGACCTGCTGCTGGCCAAGGACCTGGTGCAAGTCGAAATCCGCAAGCACGC
TCCCGGCGCGCGCCACGAGCATTGGCTGGCGCAACCCGAGTTGCCGCTCAACCCTGAGCAACGGGCCGCTTATGAAGCGA
TTCGCGCCGGGTTCGACAGTTATCACGCGTTCCTGCTGGCCGGCGTCACCGGCAGCGGCAAGACCGAAGTCTATTTGCAG
CTGATTCGTGAAACCCTCGAAGCCGGCAAGCAAGCACTGGTGTTGATCCCGGAGATCAACCTCGGCCCGCAAACCCTGGC
GCGCTTCGAGCAACGATTCAATGCGCGCATCGCGCTGATCCACTCGGCGGTCAACGACCGCGAGCGTCTTGAAGCCTGGC
TCGCCGCCCGGGATGGCGACGCCGACATTATTATCGGCACCCGCTCGGCGCTGTTCACACCGATGAAAAATCCCGGCCTG
ATCATCATCGACGAAGAGCACGACGGCTCCTATAAACAGCAGGAAGGCCTGCGCTATCACGCCCGCGACCTGGCATTGGT
GCGCGCACGACAGGAAAACATCCCGATCGTGCTCGGCTCTGCCACGCCTTCGCTGGAAAGCCTGCACAACGCCTACACCG
GTCGGTACGGCCTCCTACGGCTGAACGAGCGGGCGGGCGGCGCGAAGCAACCGCGCTTCCTTCGCCTGGATGTGAAAAGT
CGCCCGCTCGACAGCGGTATTTCCGGGCCAATGCAGCAAGCCATTGGCCAGACACTCGCAGCCGGCCAACAAGTGTTGGT
GTTTCTCAACCGTCGAGGTTTCGCGCCGACGCTGTTGTGCCACGACTGTGGCTGGATGTCCGAATGCCAGCGCTGCGACG
CGCGGATGACCGTGCACCAGCGCTCCGGCGAACTGCGCTGCCACCATTGCGGCTACGTCGAACGCGTGCCCCGGCACTGT
CCGAAATGCGGCAAGGTCGACTTGCGACCCGTCGGCGCCGGCACCGAACGTGCCGAAGAACGCCTGGCCATCCTGTTTCC
GGACGTGCCGGTGCTGCGGGTCGACCGCGACAGCACATCGCGCAAGGACGCGATGAATCAGTTGTTCGCGACCATTCAGA
AGGGTCAGCCATGCATTCTGGTGGGCACGCAGATGCTTGCCAAAGGGCATCACTTTCCACGAGTAACGCTGGTGTCGATT
CTCGATGCCGACGGCGGACTGTTCTCCGGCGACTTCCGCGCCAGCGAGCGCATGGCGCAACTGATCGTTCAGGTGGCCGG
ACGTGCGGGCCGGGCTGAAGAGCCGGGCAAGGTGATTATCCAGACGCATCTGGCGGATCATCCGCTGCTGGTGCAATTGA
CCGAACAAGGTTACTTCGCCTTTGCCGAGCAGGCGCTGAGCGAGCGGCGTTCTGCCGGTTTGCCGCCATTTGCACATTTG
GCGCTGCTGCGGGCGGAAGCGCACAAACCGGGGCAGGCGGAAGGGTTTCTGGATGAAGCATGCAGTGAGGCTGAACGGTT
GCTGGCCGAACAGAACCTGACCGGGATCGAGCTGCTAGGGCCAGTGCCGGCGCCGATGGAACGCCGGGCCGGGCGTTATC
GCGCGCAGTTGTTACTGCAAGCGACGGCGCGGGCGCCGCTGCATCGGTTACTGGCCAATTGGTTGCTGGTACTGGAGCAG
ATGCCGAGCGGGCGGGCGGTGCGCTGGTCGCTGGATGTCGACCCTGTAGATTTATACTGA

Domains

Predicted by InterProScan.

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)

DEAD/DEAH box helicase (DEAD)

(206-367)

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)

Primosomal protein N C-terminal domain (PriA_C)

(636-735)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

46.496

100

0.467

Multiple sequence alignment