Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   SR36_RS16425 Genome accession   NZ_CP010816
Coordinates   3294942..3295619 (+) Length   225 a.a.
NCBI ID   WP_000178163.1    Uniprot ID   -
Organism   Escherichia coli strain BL21 (TaKaRa)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3289942..3300619
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SR36_RS16390 (SR36_16390) gspG 3290422..3290859 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  SR36_RS16395 (SR36_16395) gspH 3290867..3291376 (+) 510 WP_001076029.1 type II secretion system minor pseudopilin GspH -
  SR36_RS16400 (SR36_16400) gspI 3291373..3291750 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  SR36_RS16405 (SR36_16405) gspJ 3291743..3292330 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  SR36_RS16410 (SR36_16410) gspK 3292323..3293306 (+) 984 WP_001058521.1 type II secretion system minor pseudopilin GspK -
  SR36_RS16415 (SR36_16415) gspL 3293321..3294484 (+) 1164 WP_001115228.1 type II secretion system protein GspL -
  SR36_RS16420 (SR36_16420) gspM 3294481..3294942 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  SR36_RS16425 (SR36_16425) pilD 3294942..3295619 (+) 678 WP_000178163.1 prepilin peptidase GspO Machinery gene
  SR36_RS16430 (SR36_16430) bfr 3295648..3296124 (-) 477 WP_000675504.1 bacterioferritin -
  SR36_RS16435 (SR36_16435) bfd 3296196..3296390 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  SR36_RS16440 (SR36_16440) - 3296559..3298619 (-) 2061 WP_000773160.1 carbohydrate-binding protein -
  SR36_RS16445 (SR36_16445) tuf 3298911..3300095 (-) 1185 WP_000031783.1 elongation factor Tu -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24928.81 Da        Isoelectric Point: 7.9563

>NTDB_id=124615 SR36_RS16425 WP_000178163.1 3294942..3295619(+) (pilD) [Escherichia coli strain BL21 (TaKaRa)]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTSAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=124615 SR36_RS16425 WP_000178163.1 3294942..3295619(+) (pilD) [Escherichia coli strain BL21 (TaKaRa)]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTAGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment