Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   VAA049_RS01175 Genome accession   NZ_CP010811
Coordinates   245270..247474 (+) Length   734 a.a.
NCBI ID   WP_046121311.1    Uniprot ID   -
Organism   Vibrio cholerae strain 1154-74     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 240270..252474
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VAA049_RS01155 (VAA049_221) - 241350..242516 (-) 1167 WP_046121310.1 O-succinylhomoserine (thiol)-lyase -
  VAA049_RS01160 (VAA049_222) metJ 242784..243101 (+) 318 WP_000796267.1 met regulon transcriptional regulator MetJ -
  VAA049_RS01165 (VAA049_223) - 243212..244489 (-) 1278 WP_001278591.1 malic enzyme-like NAD(P)-binding protein -
  VAA049_RS01170 (VAA049_224) rpmE 244797..245015 (-) 219 WP_000643446.1 50S ribosomal protein L31 -
  VAA049_RS01175 (VAA049_225) priA 245270..247474 (+) 2205 WP_046121311.1 primosomal protein N' Machinery gene
  VAA049_RS01180 (VAA049_226) cytR 247780..248787 (+) 1008 WP_046121312.1 DNA-binding transcriptional regulator CytR Regulator
  VAA049_RS01185 (VAA049_227) - 248958..249509 (+) 552 WP_046121313.1 SPOR domain-containing protein -
  VAA049_RS01190 (VAA049_228) hslV 249646..250203 (+) 558 WP_000208249.1 ATP-dependent protease subunit HslV -
  VAA049_RS01195 (VAA049_229) hslU 250269..251600 (+) 1332 WP_001293366.1 HslU--HslV peptidase ATPase subunit -

Sequence

Protein


Download         Length: 734 a.a.        Molecular weight: 81999.32 Da        Isoelectric Point: 8.3461

>NTDB_id=124378 VAA049_RS01175 WP_046121311.1 245270..247474(+) (priA) [Vibrio cholerae strain 1154-74]
MRPSIARVCLPVPLDKSFDYLIPAHLFPVLGGRVQVPFGRQTLVGIVQSLTHQSDFPIEQLKSVQAVLDDAPVWPDKLQS
LLHWCSQFYHYPLGETYANALPGALRKGKAAELTSHKEWRLTALGQEQLMQGVKRGAVKQAQVLHLLQHGALSHQTLLDE
EVSSTTLKALVEKGWIECEERKPVARPWPQELEAKVDKPKLNQEQAIAIAAVNSQQGFGCFLLEGVTGSGKTEVYLNLIT
PVLACGEQALVLVPEIGLTPQTINRFRQRFNVPVEVMHSALNDTERLNAWLAARDKVAGIVIGTRSALLTPFAKLGIIIV
DEEHDSSYKQQDSLRYHARDVAVMRAHLENIPIVLGSATPALETLHNALSGKYHHLQLTQRAGNALPTRNHALDVKGLYL
ESGLSAPLIAEMRRHLSAGNQVMLFLNRRGFSPALMCHECGWIAECQRCDAYYTYHQHSNEMRCHHCGSQRPVLHQCKGC
GSTQLVTVGVGTEQLEAQLHTLFPEYRSVRIDRDSTRRKGSLESALTAIRKGEYQILIGTQMLAKGHHFPDVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVVLQTHHPEHSLLQALLHKDYHHFALTALEERKLAQLPPYSFLSLF
RAEANHTAQVEDFLRQVRETLRCNPWFDSECMVLGPTPAPLAKRAGKFRWQLLLQTPNRTLMQKILQSARPALQQLPLAS
KVRWSIDIDPQDLS

Nucleotide


Download         Length: 2205 bp        

>NTDB_id=124378 VAA049_RS01175 WP_046121311.1 245270..247474(+) (priA) [Vibrio cholerae strain 1154-74]
ATGCGACCCTCCATTGCCCGAGTTTGCCTACCTGTACCTCTGGATAAGTCTTTCGACTATCTGATCCCAGCTCATCTATT
TCCAGTGCTGGGCGGTCGTGTGCAAGTGCCTTTTGGGCGACAAACCTTGGTCGGTATTGTCCAGTCGCTGACACATCAAT
CCGATTTTCCTATCGAACAACTGAAATCCGTGCAAGCCGTTTTGGATGACGCGCCCGTGTGGCCAGATAAACTGCAGTCT
CTGCTGCACTGGTGTAGCCAGTTTTATCATTATCCGCTCGGTGAAACGTACGCGAATGCCTTGCCCGGCGCATTGCGCAA
AGGCAAAGCGGCAGAGCTGACTAGCCATAAAGAGTGGCGTTTGACCGCGCTTGGTCAAGAGCAACTGATGCAAGGGGTGA
AGCGTGGCGCGGTAAAACAAGCACAAGTTCTCCATTTGTTGCAGCACGGCGCTTTATCGCACCAAACTTTGCTTGATGAA
GAGGTGAGCAGTACGACCCTCAAAGCCTTGGTCGAAAAAGGGTGGATTGAGTGTGAAGAGCGTAAACCTGTGGCGCGTCC
TTGGCCGCAAGAGCTGGAAGCTAAAGTCGATAAACCTAAGCTTAATCAAGAGCAGGCGATCGCGATTGCGGCGGTGAATA
GCCAGCAAGGTTTCGGCTGCTTTTTATTAGAAGGGGTAACGGGCTCAGGCAAAACAGAGGTGTATCTTAACCTCATCACC
CCTGTATTGGCGTGTGGTGAACAAGCCTTGGTGTTGGTGCCGGAAATTGGTTTAACTCCACAAACCATCAATCGTTTTCG
CCAACGTTTTAATGTCCCCGTCGAAGTGATGCACTCGGCGCTCAACGACACTGAACGCCTCAACGCTTGGCTGGCGGCGC
GCGATAAAGTGGCGGGGATTGTGATTGGTACGCGCTCGGCGCTGCTCACACCCTTTGCCAAATTGGGCATTATTATTGTT
GATGAAGAACATGATAGTTCTTACAAGCAGCAAGATAGCCTGCGTTATCACGCCCGAGATGTGGCTGTGATGCGCGCGCA
TTTGGAGAATATTCCGATTGTGCTCGGCTCGGCGACGCCAGCCTTGGAAACGTTGCATAATGCGCTCAGTGGTAAATACC
ATCACTTGCAACTGACGCAGCGCGCCGGTAATGCACTACCCACACGTAACCATGCGTTGGATGTGAAAGGGCTCTATCTG
GAAAGTGGCCTGTCTGCGCCTTTGATTGCGGAAATGCGCCGCCATTTGTCGGCGGGCAATCAGGTGATGCTGTTTTTAAA
CCGCCGCGGTTTTTCTCCGGCCTTGATGTGTCACGAGTGTGGCTGGATTGCGGAGTGCCAGCGCTGTGATGCCTATTACA
CCTACCATCAACACAGCAATGAAATGCGCTGCCACCATTGTGGTTCACAGCGTCCTGTTCTCCATCAATGCAAAGGGTGC
GGCTCGACTCAACTGGTTACTGTCGGCGTTGGTACGGAACAGCTTGAAGCGCAATTACACACTCTATTCCCTGAGTATCG
TTCAGTGCGGATTGATCGAGATAGCACGCGGCGTAAAGGCAGTTTGGAATCGGCGTTAACCGCGATTCGCAAAGGCGAAT
ATCAGATTTTGATTGGTACGCAAATGCTCGCCAAAGGACATCACTTCCCCGATGTTACCTTGGTCGCGTTGTTGGATGTC
GATGGCTCATTGTACAGCAGTGATTTTCGCGCTTCTGAGCGCTTGGCTCAGCTCTTTATTCAAGTCGCAGGGCGGGCAGG
GCGTGCCAGCAAGCCGGGCGAAGTGGTGCTACAAACGCATCATCCCGAACACAGTTTGCTACAAGCCTTGCTGCATAAGG
ATTACCACCATTTTGCCCTGACGGCGTTGGAAGAGCGTAAGCTGGCGCAGTTGCCCCCTTATTCTTTCTTGAGCTTATTT
CGAGCTGAAGCCAATCACACCGCTCAAGTGGAAGATTTTTTGCGTCAAGTGCGCGAAACCTTACGCTGCAATCCTTGGTT
TGATAGCGAATGTATGGTGCTCGGGCCAACACCTGCGCCACTCGCGAAACGAGCGGGAAAATTCCGTTGGCAACTTTTAC
TACAAACGCCGAATCGCACCCTGATGCAGAAGATTTTGCAAAGCGCACGTCCAGCACTGCAACAATTACCGCTGGCGAGC
AAGGTGCGCTGGTCGATCGATATTGATCCGCAAGATTTGAGCTAA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(205-364)

Helicase conserved C-terminal domain (Helicase_C)

(515-589)

3DNA-binding domain (3BD) (PriA_3primeBD)

(7-102)

Primosomal protein N C-terminal domain (PriA_C)

(633-730)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(446-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

43.572

100

0.439