Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   SS17_RS22050 Genome accession   NZ_CP008805
Coordinates   4222335..4223459 (-) Length   374 a.a.
NCBI ID   WP_000228545.1    Uniprot ID   A0AAV3H2U0
Organism   Escherichia coli O157:H7 str. SS17     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4217335..4228459
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SS17_RS22020 (SS17_4299) yrdA 4219135..4219689 (+) 555 WP_001286216.1 gamma carbonic anhydrase family protein -
  SS17_RS22025 yrdB 4219665..4219922 (-) 258 WP_001070590.1 DUF1488 domain-containing protein -
  SS17_RS22030 (SS17_4300) aroE 4219919..4220737 (-) 819 WP_000451254.1 shikimate dehydrogenase -
  SS17_RS22035 (SS17_4301) tsaC 4220742..4221314 (-) 573 WP_001301437.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  SS17_RS22040 (SS17_4302) yrdD 4221319..4221861 (-) 543 WP_001129722.1 type I DNA topoisomerase -
  SS17_RS22045 (SS17_4303) smg 4221890..4222363 (-) 474 WP_000460672.1 DUF494 family protein Smg -
  SS17_RS22050 (SS17_4304) dprA 4222335..4223459 (-) 1125 WP_000228545.1 DNA-protecting protein DprA Machinery gene
  SS17_RS22055 (SS17_4305) def 4223589..4224098 (+) 510 WP_000114984.1 peptide deformylase -
  SS17_RS22060 (SS17_4306) fmt 4224113..4225060 (+) 948 WP_000004459.1 methionyl-tRNA formyltransferase -
  SS17_RS22065 (SS17_4307) rsmB 4225106..4226395 (+) 1290 WP_001301795.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  SS17_RS22070 (SS17_4308) trkA 4226417..4227793 (+) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  SS17_RS22075 (SS17_4309) mscL 4227923..4228333 (+) 411 WP_000022442.1 large-conductance mechanosensitive channel protein MscL -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 41034.02 Da        Isoelectric Point: 6.3845

>NTDB_id=124362 SS17_RS22050 WP_000228545.1 4222335..4223459(-) (dprA) [Escherichia coli O157:H7 str. SS17]
MVDTEIWLRLMSISSLYGDDMVRIAHWLARQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLAKHGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE
VTPVDVVAERAGRPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=124362 SS17_RS22050 WP_000228545.1 4222335..4223459(-) (dprA) [Escherichia coli O157:H7 str. SS17]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGTATCAGCAGCTTGTACGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAGACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGGAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGTGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG
CGAAGCATGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTAGCGCATAAAGCAGCCTTACAGGTAAAT
GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCT
TGAACAGGGGGGCGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGTAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGAGAAGTTTTTGCCTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCGACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterproScan.

DprA winged helix domain (DprA_WH)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.78

98.663

0.481
  dprA Glaesserella parasuis strain SC1401

45.401

90.107

0.409
  dprA Vibrio cholerae strain A1552

49.355

82.888

0.409
  dprA Haemophilus influenzae Rd KW20

44.214

90.107

0.398
  dprA Legionella pneumophila strain ERS1305867

44.545

88.235

0.393

Multiple sequence alignment