Detailed information    

experimental Experimentally validated

Overview

Name   ppk1   Type   Unclear
Locus tag   CJJ81176_RS06550 Genome accession   NC_008787
Coordinates   1276066..1278174 (+) Length   702 a.a.
NCBI ID   WP_223209681.1    Uniprot ID   -
Organism   Campylobacter jejuni subsp. jejuni 81-176     
Function   require for natural transformation   
Unclear

Function

The Deltappk1 mutant showed a decreased frequency of natural transformation and an increased susceptibility to various antimicrobials.


Genomic Context

Location: 1271066..1283174
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJJ81176_RS06520 (CJJ81176_1355) - 1271987..1272208 (+) 222 WP_002856036.1 hypothetical protein -
  CJJ81176_RS06525 (CJJ81176_1356) - 1272209..1272490 (+) 282 WP_009882907.1 type II toxin-antitoxin system RelE/ParE family toxin -
  CJJ81176_RS06535 (CJJ81176_1358) - 1272850..1273404 (-) 555 WP_002855904.1 membrane protein -
  CJJ81176_RS06540 (CJJ81176_1359) nrfA 1273540..1275372 (-) 1833 WP_002869320.1 ammonia-forming cytochrome c nitrite reductase subunit c552 -
  CJJ81176_RS06545 (CJJ81176_1360) nrfH 1275387..1275902 (-) 516 WP_010891930.1 cytochrome c nitrite reductase small subunit -
  CJJ81176_RS06550 (CJJ81176_1361) ppk1 1276066..1278174 (+) 2109 WP_223209681.1 RNA degradosome polyphosphate kinase Unclear
  CJJ81176_RS06555 (CJJ81176_1363) - 1278618..1279028 (-) 411 WP_002783915.1 hypothetical protein -
  CJJ81176_RS06560 (CJJ81176_1364) ruvB 1279119..1280126 (+) 1008 WP_002856258.1 Holliday junction branch migration DNA helicase RuvB -
  CJJ81176_RS06565 (CJJ81176_1365) amaA 1280130..1281173 (+) 1044 WP_002856044.1 AI-2E family transporter -
  CJJ81176_RS06570 (CJJ81176_1366) fumC 1281203..1282594 (-) 1392 WP_002856014.1 class II fumarate hydratase -

Sequence

Protein


Download         Length: 702 a.a.        Molecular weight: 81458.96 Da        Isoelectric Point: 7.2556

>NTDB_id=1243 CJJ81176_RS06550 WP_223209681.1 1276066..1278174(+) (ppk1) [Campylobacter jejuni subsp. jejuni 81-176]
MQLKKEKLMQTSPDMFINRELSWLRFNSRVLDQCSKNLPLLEKLKFIAIYCTNLDEFYMIRVAGLKQLFSAGVNASSSDE
MTPLQQLKAIRKYLHQEKELLERYFNEITSELEKENLFIKHYENLDENLKQKCDEYFFSNIFPVIVPIAVDATHPFPHLN
NLSFSLAVKICDKAHPELVKFGMIRIPRVLPRFYEVSANIYVPIESIVHQHAEEIFPGYKLLASAAFRVTRNADMVIEEE
EADDFMMILEQGLKLRRKGAFVRLQIQKDADEQIVEFLNTHMKIFHKDVYEYSILLNLPSLWQIAGNKTFTHLLSPLYTP
KTLPPFDENLSIFDAVEKEDILIIQPFESFDPVYKFIKEASKDPEVISIRMTLYRVEKNSNIVQALIDAASDGKQVTVMV
ELKARFDEENNLHWAKALENAGAHVIYGITGFKVHAKVSQVIRKQGDKLKFYMHLSTGNYNASSAKIYTDVSYFTSKAEF
ARDTTSFFHILSGFSKNRRLQTLSMSPNQIKEKVLEMIRIETSKKNEGVIVAKMNSLVDSDIIQALYEASMEGVQIDLII
RGICCLKPDEEYSKNIRVRSIIGKYLEHARVFYFKHSEPNYFISSADWMPRNLERRLELMTPIYDERSKAKLAQFLRLQL
SDNVLAYELKNNGEYEKIPSSEKIIDSQQTLEEYVSKIYKTLKKDTDQSRATHLASKLFKEN

Nucleotide


Download         Length: 2109 bp        

>NTDB_id=1243 CJJ81176_RS06550 WP_223209681.1 1276066..1278174(+) (ppk1) [Campylobacter jejuni subsp. jejuni 81-176]
ATACAACTAAAAAAGGAAAAATTAATGCAAACAAGTCCTGATATGTTTATCAATCGCGAACTTTCTTGGCTTCGCTTTAA
TTCTCGTGTTTTAGACCAATGTTCTAAAAATTTACCCTTGTTAGAAAAATTAAAATTTATAGCTATATATTGCACTAATT
TAGATGAATTTTATATGATACGCGTCGCAGGCTTAAAACAACTTTTTTCAGCTGGAGTTAATGCAAGCAGTAGCGATGAA
ATGACCCCGTTACAACAATTAAAAGCCATACGCAAATATTTACACCAAGAAAAAGAACTTTTGGAGCGTTATTTTAATGA
AATCACAAGTGAATTAGAAAAAGAAAATCTTTTCATAAAACATTATGAGAATTTAGATGAAAATTTAAAACAAAAATGTG
ATGAGTATTTTTTCTCTAATATTTTTCCTGTTATTGTTCCAATAGCTGTTGATGCTACTCACCCTTTTCCGCATTTAAAC
AACTTATCTTTTTCACTAGCGGTTAAAATTTGCGATAAAGCGCATCCTGAACTTGTAAAATTTGGAATGATTAGAATTCC
AAGAGTTTTACCTCGTTTTTATGAAGTAAGTGCAAATATTTATGTTCCTATAGAAAGTATAGTCCATCAACACGCAGAAG
AAATTTTTCCAGGCTATAAACTCTTAGCTTCAGCAGCATTTAGAGTGACTAGAAATGCAGATATGGTAATAGAAGAGGAA
GAAGCTGATGATTTTATGATGATTTTAGAACAAGGCTTGAAGCTTCGCAGAAAAGGAGCTTTTGTAAGATTGCAAATTCA
AAAAGATGCAGATGAGCAAATCGTAGAATTTCTTAATACTCACATGAAAATTTTTCATAAAGATGTTTATGAATATTCTA
TTTTACTCAATCTCCCTAGCCTTTGGCAAATCGCAGGAAATAAAACCTTTACGCATCTTTTAAGCCCACTTTACACGCCT
AAAACTTTACCACCTTTTGATGAGAATTTATCTATTTTTGATGCTGTAGAAAAAGAAGATATACTCATTATACAACCTTT
TGAAAGTTTTGATCCAGTTTATAAATTTATCAAAGAAGCAAGCAAAGATCCTGAAGTAATTTCCATAAGAATGACACTTT
ATAGAGTTGAAAAAAATTCCAATATAGTTCAAGCTTTAATTGATGCTGCAAGCGATGGAAAACAAGTAACTGTGATGGTA
GAATTAAAAGCACGCTTTGATGAAGAAAACAATCTTCATTGGGCAAAAGCTTTAGAAAATGCCGGAGCTCATGTTATTTA
TGGCATTACAGGTTTTAAAGTCCACGCTAAGGTTTCTCAAGTCATACGCAAACAAGGAGATAAACTTAAATTTTATATGC
ATTTAAGTACGGGAAATTATAATGCAAGCAGTGCTAAAATCTATACTGATGTGAGTTATTTTACAAGCAAAGCAGAATTT
GCAAGAGATACGACAAGCTTTTTTCACATCTTATCGGGCTTTAGCAAAAATCGTCGCCTTCAAACTCTTTCAATGAGCCC
AAATCAAATCAAAGAAAAAGTTTTAGAAATGATACGCATTGAAACAAGCAAGAAAAATGAAGGTGTGATTGTAGCTAAAA
TGAATTCTCTTGTAGATAGTGATATCATACAAGCGCTTTATGAAGCAAGTATGGAAGGAGTGCAAATCGATCTTATCATA
CGCGGAATTTGTTGCTTAAAACCTGATGAAGAATACAGCAAAAACATACGCGTTAGAAGCATCATAGGAAAATACTTAGA
ACATGCTAGAGTATTTTATTTTAAACATAGTGAGCCAAATTATTTTATATCGAGTGCAGATTGGATGCCAAGAAATTTAG
AGCGTCGCTTAGAACTTATGACTCCTATATATGATGAAAGAAGTAAAGCTAAATTAGCTCAATTTTTACGCTTGCAACTT
AGTGATAATGTTTTAGCTTATGAGCTTAAAAACAATGGAGAGTATGAAAAAATTCCTTCTTCTGAAAAAATCATAGATTC
TCAACAAACCTTAGAAGAATATGTAAGTAAAATTTATAAAACACTTAAAAAAGATACAGATCAAAGTCGTGCAACGCACT
TAGCTTCCAAACTTTTTAAGGAAAACTAA

Domains

Predicted by InterproScan.

Polyphosphate kinase N-terminal domain (PP_kinase_N)

(16-119)

Polyphosphate kinase C-terminal domain 2 (PP_kinase_C)

(503-663)

Polyphosphate kinase middle domain (PP_kinase)

(130-322)

Polyphosphate kinase C-terminal domain 1 (PP_kinase_C_1)

(331-496)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Dharanesh Gangaiah et al. (2009) Importance of polyphosphate kinase 1 for Campylobacter jejuni viable-but-nonculturable cell formation, natural transformation, and antimicrobial resistance. Applied And Environmental Microbiology 75(24):7838-49. [PMID: 19837830]