Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   TH69_RS16630 Genome accession   NZ_CP010585
Coordinates   3325777..3326454 (+) Length   225 a.a.
NCBI ID   WP_000178163.1    Uniprot ID   -
Organism   Escherichia coli strain C41(DE3)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3320777..3331454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TH69_RS16595 (TH69_16595) gspG 3321257..3321694 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  TH69_RS16600 (TH69_16600) gspH 3321702..3322211 (+) 510 WP_001076029.1 type II secretion system minor pseudopilin GspH -
  TH69_RS16605 (TH69_16605) gspI 3322208..3322585 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  TH69_RS16610 (TH69_16610) gspJ 3322578..3323165 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  TH69_RS16615 (TH69_16615) gspK 3323158..3324141 (+) 984 WP_001058521.1 type II secretion system minor pseudopilin GspK -
  TH69_RS16620 (TH69_16620) gspL 3324156..3325319 (+) 1164 WP_001115228.1 type II secretion system protein GspL -
  TH69_RS16625 (TH69_16625) gspM 3325316..3325777 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  TH69_RS16630 (TH69_16630) pilD 3325777..3326454 (+) 678 WP_000178163.1 prepilin peptidase GspO Machinery gene
  TH69_RS16635 (TH69_16635) bfr 3326483..3326959 (-) 477 WP_000675504.1 bacterioferritin -
  TH69_RS16640 (TH69_16640) bfd 3327031..3327225 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  TH69_RS16645 (TH69_16645) - 3327394..3329454 (-) 2061 WP_000773160.1 carbohydrate-binding protein -
  TH69_RS16650 (TH69_16650) tuf 3329746..3330930 (-) 1185 WP_000031783.1 elongation factor Tu -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24928.81 Da        Isoelectric Point: 7.9563

>NTDB_id=124189 TH69_RS16630 WP_000178163.1 3325777..3326454(+) (pilD) [Escherichia coli strain C41(DE3)]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTSAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=124189 TH69_RS16630 WP_000178163.1 3325777..3326454(+) (pilD) [Escherichia coli strain C41(DE3)]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTAGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment