Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   DR64_RS08775 Genome accession   NZ_CP008760
Coordinates   1972008..1973525 (+) Length   505 a.a.
NCBI ID   WP_011486707.1    Uniprot ID   Q145W1
Organism   Paraburkholderia xenovorans LB400     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1967008..1978525
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DR64_RS08750 (DR64_1793) kdsD 1968178..1969161 (+) 984 WP_011486712.1 arabinose 5-phosphate isomerase KdsD -
  DR64_RS08755 (DR64_1794) - 1969161..1969721 (+) 561 WP_011486711.1 HAD family hydrolase -
  DR64_RS08760 (DR64_1795) lptC 1969718..1970326 (+) 609 WP_011486710.1 LPS export ABC transporter periplasmic protein LptC -
  DR64_RS08765 (DR64_1796) lptA 1970347..1971018 (+) 672 WP_011486709.1 lipopolysaccharide transport periplasmic protein LptA -
  DR64_RS08770 (DR64_1797) lptB 1971015..1971800 (+) 786 WP_011486708.1 LPS export ABC transporter ATP-binding protein -
  DR64_RS08775 (DR64_1798) rpoN/rpoN1 1972008..1973525 (+) 1518 WP_011486707.1 RNA polymerase factor sigma-54 Machinery gene
  DR64_RS08780 (DR64_1799) hpf 1973774..1974133 (+) 360 WP_007179410.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  DR64_RS08785 (DR64_1800) ptsN 1974423..1974938 (+) 516 WP_007179411.1 PTS IIA-like nitrogen regulatory protein PtsN -
  DR64_RS08790 (DR64_1801) hprK 1975078..1976046 (+) 969 WP_011486706.1 HPr(Ser) kinase/phosphatase -
  DR64_RS08795 (DR64_1802) rapZ 1976143..1977036 (+) 894 WP_038458391.1 RNase adapter RapZ -
  DR64_RS08800 (DR64_1803) - 1977076..1977708 (+) 633 WP_011486704.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 505 a.a.        Molecular weight: 55379.50 Da        Isoelectric Point: 5.0733

>NTDB_id=124093 DR64_RS08775 WP_011486707.1 1972008..1973525(+) (rpoN/rpoN1) [Paraburkholderia xenovorans LB400]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAISQNPLLENEDDWIASPLRVAADGSLIAQAPNSSAPPDQM
GGSTSSSSTSSSERAENGEPQGVDEYNGLAGDSNGDASQWNLDDYGRSGNASDDDDLPPLQIHESSTSLRDHLMAQLRVT
QASPRDRALVTFLIESLDDDGYLAATLEEVLADLPEELEVDLDELNAALALLHSFDPAGVGARSASECLKLQLLRLDASP
TRTLALEIVAHHLELLAARDFTRLRKHLKASDDDLRDAHVLIRSLEPFPGAAYGKAEADYVVPDIMVRKTAQGWQAELNP
EVVPKLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQKSFFVHGEIAMRPLVLREIA
DTLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAENPKSPLSDSRIAELLAEQGF
VVARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1518 bp        

>NTDB_id=124093 DR64_RS08775 WP_011486707.1 1972008..1973525(+) (rpoN/rpoN1) [Paraburkholderia xenovorans LB400]
ATGAAAGCCAGCCTCCAACTCCGCCTATCGCAGCATCTTGCGCTGACGCCGCAACTGCAGCAGTCCATCCGGCTGCTTCA
GTTGTCCACGCTCGAATTGCAGCAGGAAGTCGCAATGGCGATCTCGCAGAATCCGCTCCTCGAGAACGAGGACGACTGGA
TCGCGAGCCCGCTGCGCGTGGCGGCCGACGGCTCGCTGATCGCCCAGGCGCCGAATTCCTCCGCTCCGCCCGACCAGATG
GGCGGCAGCACGTCGTCATCGTCCACCAGCAGCAGCGAGCGCGCCGAGAACGGCGAGCCGCAGGGCGTGGACGAATACAA
CGGCCTCGCGGGCGACAGTAACGGCGACGCGTCGCAATGGAATCTCGACGACTACGGCCGCTCGGGCAACGCCTCGGACG
ACGACGACCTGCCGCCGCTGCAAATCCACGAATCGAGCACCTCGCTGCGCGATCACCTGATGGCGCAATTGCGCGTCACC
CAGGCGAGTCCGCGCGACCGCGCGCTGGTCACGTTCCTGATCGAGTCGCTCGACGACGACGGCTACCTTGCCGCCACGCT
CGAAGAAGTGCTGGCGGATCTGCCCGAAGAGCTCGAAGTCGACCTCGACGAGCTGAATGCCGCGCTTGCGCTGCTGCATA
GCTTCGACCCGGCCGGGGTCGGCGCGCGCTCCGCGTCGGAATGTCTCAAGCTGCAATTGCTGCGACTCGATGCCTCACCT
ACCCGCACGCTTGCGCTCGAAATCGTAGCGCACCACCTGGAATTGCTCGCGGCGCGCGACTTCACGCGCCTGCGCAAACA
TCTGAAAGCCAGCGACGACGACCTGCGCGATGCACACGTGCTGATCCGCTCGCTCGAACCGTTCCCCGGCGCCGCCTACG
GCAAGGCCGAAGCGGACTATGTCGTGCCGGACATCATGGTGCGTAAAACAGCACAGGGCTGGCAGGCCGAGCTGAACCCG
GAAGTCGTGCCCAAGCTGCGTATCAACCATCTTTACGCGAATATCCTGCGCAATAACCGGGGCGATCCGGGCAGCGGTTC
GTTGCGCCAGCAACTGCAGGAAGCGCGCTGGCTGATCAAAAATATCCAGCAGCGTTTCGAGACGATCCTCCGGGTGGCGC
AAGCCATTGTGGAGCGTCAAAAGAGCTTTTTTGTGCATGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATTGCT
GATACGCTGGGCCTACACGAGTCAACTGTCTCGCGTGTGACAACCGGCAAATACATGCTGACCCCATTCGGGACGCTTGA
ATTTAAGTACTTCTTCGGATCACACGTTTCGACTGACACGGGCGGCGCGGCGTCTTCAACGGCGATCCGTGCGCTCATCA
AGCAACTGATAGGAGCGGAAAACCCGAAATCTCCTCTTTCAGACAGCCGCATAGCCGAACTGCTGGCGGAACAGGGCTTC
GTGGTCGCACGGCGTACCGTCGCGAAATATCGTGAAGCGCTCAAGATTCCGGCAGTCAACCTGCGCAAGTCGTTATAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(348-503)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(141-332)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q145W1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.525

100

0.675

Multiple sequence alignment