Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   DP46_RS22920 Genome accession   NZ_CP008755
Coordinates   1303940..1305448 (+) Length   502 a.a.
NCBI ID   WP_004548100.1    Uniprot ID   -
Organism   Burkholderia pseudomallei strain BEK     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1298940..1310448
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DP46_RS22895 (DP46_1118) kdsD 1300172..1301155 (+) 984 WP_004530936.1 arabinose 5-phosphate isomerase KdsD -
  DP46_RS22900 (DP46_1119) - 1301155..1301694 (+) 540 WP_004200146.1 HAD family hydrolase -
  DP46_RS22905 (DP46_1120) lptC 1301700..1302314 (+) 615 WP_004548084.1 LPS export ABC transporter periplasmic protein LptC -
  DP46_RS22910 (DP46_1121) lptA 1302333..1302998 (+) 666 WP_004195213.1 lipopolysaccharide transport periplasmic protein LptA -
  DP46_RS22915 (DP46_1122) lptB 1302995..1303771 (+) 777 WP_004195214.1 LPS export ABC transporter ATP-binding protein -
  DP46_RS22920 (DP46_1124) rpoN/rpoN1 1303940..1305448 (+) 1509 WP_004548100.1 RNA polymerase factor sigma-54 Machinery gene
  DP46_RS22930 (DP46_1125) raiA 1305598..1305957 (+) 360 WP_004195219.1 ribosome-associated translation inhibitor RaiA -
  DP46_RS22935 (DP46_1126) ptsN 1306189..1306698 (+) 510 WP_004534308.1 PTS IIA-like nitrogen regulatory protein PtsN -
  DP46_RS22940 (DP46_1127) hprK 1306827..1307795 (+) 969 WP_004195225.1 HPr(Ser) kinase/phosphatase -
  DP46_RS22945 (DP46_1128) rapZ 1307881..1308774 (+) 894 WP_004530937.1 RNase adapter RapZ -
  DP46_RS22950 (DP46_1129) - 1308801..1309433 (+) 633 WP_004195230.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 54923.06 Da        Isoelectric Point: 4.8740

>NTDB_id=124057 DP46_RS22920 WP_004548100.1 1303940..1305448(+) (rpoN/rpoN1) [Burkholderia pseudomallei strain BEK]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLENDDDWIASPLRVAADGSVIAQTPASSAPEPMA
GAPNGTAATNERTERDETAGVDEYDSYSADGGDASQWNLDDFGRSPAASDDDDLPPMQIHETSTSLRDHLMAQLRVTQAS
PRDRALVTFLIESLDDDGYLSASLEEVLADLPAELEVDVDELNAALALVHSFDPAGVGARSASECLKLQLLRLDPSPTRA
LALDIVSQHLELLAARDFTRLRKQLKATDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQNWQAELNPEVV
PKLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTL
GLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRAFIKQLIGAEDPKSPLSDSRIAELLAEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=124057 DP46_RS22920 WP_004548100.1 1303940..1305448(+) (rpoN/rpoN1) [Burkholderia pseudomallei strain BEK]
ATGAAAGCCAGTCTTCAACTTCGCCTGTCGCAGCATCTCGCGCTCACGCCCCAGCTTCAGCAGTCGATCCGGCTGCTGCA
ACTGTCGACGCTCGAACTGCAACAGGAAGTCGCGACGGCGATCGCGCAGAACCCGCTGCTCGAGAACGACGATGACTGGA
TCGCGAGCCCGTTGCGCGTCGCGGCCGACGGCTCGGTGATCGCGCAGACGCCCGCGTCGTCCGCGCCCGAGCCGATGGCG
GGCGCGCCGAACGGCACGGCTGCGACCAATGAGCGCACCGAGCGCGACGAAACCGCCGGCGTCGACGAATACGACAGCTA
TTCGGCCGACGGCGGCGACGCGAGCCAGTGGAACCTCGACGACTTCGGCCGCTCGCCCGCCGCGTCCGACGACGACGATC
TGCCGCCGATGCAGATCCACGAGACGTCGACGTCGCTGCGCGATCATCTGATGGCGCAGTTGCGCGTCACGCAGGCGAGC
CCGCGCGATCGCGCGCTCGTCACGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGAGCGCGTCGCTCGAGGAAGT
GCTCGCCGATCTGCCGGCGGAGCTCGAGGTCGACGTCGACGAACTGAACGCGGCGCTCGCGCTCGTGCACAGCTTCGATC
CGGCGGGCGTCGGCGCGCGCTCCGCGTCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTGGATCCGTCCCCGACGCGCGCG
CTCGCGCTCGATATCGTGTCGCAGCATCTGGAGCTGCTCGCCGCGCGCGATTTCACGCGCCTGCGCAAGCAACTGAAGGC
GACCGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCGGCGTACGGCAAGGCCG
AAGCCGATTACGTGGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGAACTGGCAGGCGGAACTCAATCCGGAAGTGGTG
CCGAAGCTGCGGATCAACAATCTTTACGCGAACATCCTGCGCAACAGCCGGGGCGATCCGAGCGCGGGATCGCTGAAGCA
ACAGCTTCAGGAAGCGCGCTGGCTGATCAAGAACATTCAGCAGCGGTTCGAGACGATCCTGCGCGTCGCGCAGGCGATTG
TCGAGCGTCAGAAGAATTTCTTCGCGCACGGCGAAATTGCGATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGCCTACACGAGTCGACTGTCTCCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGCACACTTGAATTTAAGTA
CTTCTTCGGATCGCACGTATCGACAGACACCGGAGGTGCCGCGTCGTCGACAGCGATCCGCGCCTTCATCAAACAACTGA
TAGGAGCCGAAGACCCCAAATCCCCTCTTTCGGACAGTCGCATAGCCGAGCTGCTAGCGGAACAAGGGTTCGTGGTCGCG
CGCCGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCGGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-500)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.515

100

0.689

Multiple sequence alignment