Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   PSA3335_RS23850 Genome accession   NZ_CP008742
Coordinates   4987794..4989386 (+) Length   530 a.a.
NCBI ID   WP_002551996.1    Uniprot ID   A0A554GKF2
Organism   Pseudomonas savastanoi pv. savastanoi NCPPB 3335     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 4982794..4994386
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSA3335_RS23830 (PSA3335_22830) pgeF 4984576..4985304 (-) 729 WP_002552000.1 peptidoglycan editing factor PgeF -
  PSA3335_RS23835 (PSA3335_22835) rluD 4985301..4986263 (-) 963 WP_002551999.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  PSA3335_RS23840 (PSA3335_22840) - 4986413..4987435 (+) 1023 WP_002551998.1 outer membrane protein assembly factor BamD -
  PSA3335_RS23845 (PSA3335_22845) - 4987565..4987804 (+) 240 WP_002551997.1 PP0621 family protein -
  PSA3335_RS23850 (PSA3335_22850) pilS 4987794..4989386 (+) 1593 WP_002551996.1 two-component system sensor histidine kinase NtrB Regulator
  PSA3335_RS23855 (PSA3335_22855) - 4989389..4990728 (+) 1340 Protein_4513 sigma-54-dependent transcriptional regulator -
  PSA3335_RS29510 - 4990925..4992276 (+) 1352 WP_095077641.1 IS3 family transposase -
  PSA3335_RS23880 (PSA3335_22870) - 4992796..4993413 (+) 618 WP_002551994.1 TetR/AcrR family transcriptional regulator -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58176.98 Da        Isoelectric Point: 7.0058

>NTDB_id=123994 PSA3335_RS23850 WP_002551996.1 4987794..4989386(+) (pilS) [Pseudomonas savastanoi pv. savastanoi NCPPB 3335]
MTADTPSLASPQAQRILRLYHLYRLTIGVVLVLLISSSLETELLQLANPNLFRSGCWLYLVFNILVVVLLERPSRQAQLF
SLAMTDAIMLSALFYAAGGPSSGIGNLLIASVAISNVLLRGRIGLLIAAVSAIGIIYLTFYISFGTPSVSNDYVQAGSLG
ALCFAAAMLVQALTARMQVSEVLAEQRAADVDSLEELNALILQRMRTGILVLDANRQVLLANQGALTLLGHERLVGQVID
AYSPQLVERLRQWLVNPIMVPRSVTTSPIGPVLQPSFAALNRGDQRQTLIFLEDLSQITHQAQQLKLAALGRLTAGIAHE
IRNPLGAVSHAAQLLNESEELSAADRRLGQIIHDQSGRIDRVIENVLQLSRRREAQPELLDLKTWLEHFVTEFRSSSAPN
QSIHVDISPGTLTTRMDSGQLTQIMTNLLQNGLRYSGRNHSSAQVWLKLHHDRQSDLPILEVQDDGPGVSEQHLAKLFEP
FFTTESKGTGLGLYLSRELCESNQAHLEYVPRQGGGSCLRITFAHPFKTS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=123994 PSA3335_RS23850 WP_002551996.1 4987794..4989386(+) (pilS) [Pseudomonas savastanoi pv. savastanoi NCPPB 3335]
GTGACCGCTGACACGCCCTCGCTTGCCAGCCCACAGGCGCAAAGGATTCTGCGGCTTTACCACCTTTATCGACTGACCAT
CGGCGTGGTACTGGTCCTGCTGATTTCCAGCAGTCTGGAAACCGAACTGTTGCAACTGGCCAATCCAAACCTGTTCCGCT
CGGGTTGCTGGCTGTATCTGGTGTTCAACATTCTGGTCGTCGTGCTGCTGGAGCGTCCCAGCCGTCAGGCGCAGCTGTTC
AGTCTGGCAATGACTGACGCAATCATGCTCTCTGCGCTGTTCTACGCGGCGGGTGGCCCTTCCAGCGGCATCGGCAATCT
GTTGATTGCTTCCGTAGCAATCAGCAATGTATTGCTGCGGGGCCGTATCGGTTTGCTGATCGCCGCCGTTTCTGCCATCG
GCATCATCTACCTGACGTTCTATATCAGCTTCGGTACACCGTCGGTTTCCAACGACTACGTGCAGGCTGGCTCTCTGGGT
GCCCTGTGCTTCGCCGCCGCAATGCTGGTGCAGGCGCTGACAGCGCGCATGCAGGTCAGCGAAGTGCTGGCCGAACAGCG
TGCAGCGGATGTCGACAGCCTTGAAGAACTCAACGCGCTGATCCTGCAACGCATGCGTACCGGCATTCTGGTACTGGATG
CCAACCGTCAGGTGCTGCTGGCCAATCAAGGTGCCCTTACCCTACTGGGCCACGAGCGACTGGTCGGCCAGGTTATCGAC
GCTTACTCGCCGCAGTTGGTGGAACGCCTGCGCCAGTGGCTCGTCAACCCGATCATGGTGCCGCGCAGCGTCACGACTTC
ACCCATCGGGCCGGTGCTGCAACCGAGCTTTGCCGCACTCAATCGTGGCGATCAGCGCCAGACGCTGATCTTTCTTGAAG
ACCTGTCGCAGATTACCCATCAGGCGCAGCAGCTCAAGCTCGCCGCGCTGGGACGCCTGACCGCCGGGATTGCCCACGAG
ATACGCAACCCGCTGGGCGCAGTCAGCCATGCCGCACAGTTACTCAACGAATCTGAAGAACTCAGCGCAGCGGACCGACG
CCTGGGGCAGATCATTCATGACCAGTCGGGGCGTATTGATCGGGTCATCGAAAATGTTCTGCAATTGTCGCGCAGACGCG
AAGCGCAGCCCGAACTGCTCGATCTGAAAACCTGGCTGGAACACTTTGTCACTGAATTTCGCAGTTCCTCTGCGCCCAAT
CAGTCGATTCATGTCGACATAAGCCCCGGCACGCTGACAACCCGCATGGATTCCGGGCAACTGACCCAGATCATGACCAA
CCTGCTACAGAACGGCCTGCGCTACAGCGGCAGAAATCATTCGTCAGCGCAGGTCTGGCTGAAGCTGCATCACGACCGAC
AAAGCGATTTGCCCATTCTGGAAGTACAGGACGACGGGCCAGGGGTCAGCGAGCAACATTTGGCCAAGCTGTTCGAGCCG
TTTTTCACCACGGAAAGCAAAGGCACCGGTCTTGGTCTATATCTGTCACGCGAGCTTTGCGAAAGCAATCAGGCGCACCT
GGAATACGTCCCGCGGCAAGGTGGCGGGAGTTGCCTGCGCATAACCTTCGCCCACCCGTTCAAAACGAGCTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-373)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A554GKF2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

61.205

100

0.613

Multiple sequence alignment