Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   DM80_RS26585 Genome accession   NZ_CP008730
Coordinates   2311594..2313099 (-) Length   501 a.a.
NCBI ID   WP_006398255.1    Uniprot ID   -
Organism   Burkholderia multivorans strain DDS 15A-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2306594..2318099
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DM80_RS26560 (DM80_2137) - 2307622..2308257 (-) 636 WP_006398250.1 LON peptidase substrate-binding domain-containing protein -
  DM80_RS26565 (DM80_2138) rapZ 2308285..2309193 (-) 909 WP_006409809.1 RNase adapter RapZ -
  DM80_RS26570 (DM80_2139) hprK 2309272..2310240 (-) 969 WP_006398252.1 HPr(Ser) kinase/phosphatase -
  DM80_RS26575 (DM80_2140) - 2310369..2310881 (-) 513 WP_035488861.1 PTS sugar transporter subunit IIA -
  DM80_RS26580 (DM80_2141) raiA 2311076..2311435 (-) 360 WP_006398254.1 ribosome-associated translation inhibitor RaiA -
  DM80_RS26585 (DM80_2142) rpoN/rpoN1 2311594..2313099 (-) 1506 WP_006398255.1 RNA polymerase factor sigma-54 Machinery gene
  DM80_RS26590 (DM80_2143) lptB 2313252..2314028 (-) 777 WP_006398256.1 LPS export ABC transporter ATP-binding protein -
  DM80_RS26595 (DM80_2144) lptA 2314025..2314708 (-) 684 WP_048805660.1 lipopolysaccharide transport periplasmic protein LptA -
  DM80_RS26600 (DM80_2145) lptC 2314729..2315343 (-) 615 WP_006415491.1 LPS export ABC transporter periplasmic protein LptC -
  DM80_RS26605 (DM80_2146) - 2315347..2315883 (-) 537 WP_006398259.1 HAD family hydrolase -
  DM80_RS26610 (DM80_2147) kdsD 2315883..2316866 (-) 984 WP_048805661.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54746.94 Da        Isoelectric Point: 4.8740

>NTDB_id=123919 DM80_RS26585 WP_006398255.1 2311594..2313099(-) (rpoN/rpoN1) [Burkholderia multivorans strain DDS 15A-1]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPVSNGEPAI
ANGNGQSSDRAERDEPAGADEYDGYAAGDASDGPQWNLDEFGRASGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTASLDEVLADLPPELEVDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKATDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKVGQGWQAELNPEVVP
KLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALRIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=123919 DM80_RS26585 WP_006398255.1 2311594..2313099(-) (rpoN/rpoN1) [Burkholderia multivorans strain DDS 15A-1]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTGGCGATGGCGGTCGCCCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTTGCGGCGGACGGCTCGCTGATCGCGCAGACGCCGCCCGTGTCGAACGGCGAGCCGGCCATC
GCGAACGGCAACGGCCAGTCGAGCGATCGTGCGGAGCGCGACGAACCGGCCGGCGCCGACGAATACGACGGCTATGCGGC
CGGCGACGCGAGCGACGGCCCGCAGTGGAACCTCGACGAATTCGGCCGCGCGAGCGGCGCCTCCGACGACGACGATCTGC
CGCCGCTGCAGGTACAGGAAGCGGCGACGTCGCTGCGCGATCATCTGTCCGCGCAGCTGCGCGTCACGCAGGCGAGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAGTCGCTCGACGACGACGGCTACCTGACCGCGTCGCTCGACGAGGTGCT
CGCCGATCTGCCGCCCGAACTCGAAGTCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGATCCGG
CCGGCGTCGGCGCGCGTTCGGCATCGGAGTGCCTGAAGCTGCAACTGCTGCGGCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAACATCTGGAGCTGCTCGCCGCGCGCGATTTCACGCGGTTGCGCAAGCATCTGAAGGCCAC
CGACGACGCACTGCGCGACGCACACGCGCTGATCCGCTCGCTCGAGCCGTTTCCCGGCGCGGCCTACGGCAAGGCCGAAG
CCGACTACGTGGTGCCCGACATCATCGTGAAGAAGGTGGGCCAGGGCTGGCAGGCCGAGCTCAATCCCGAGGTCGTGCCG
AAGCTGCGCATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGCTCGCTGAAGCAGCA
GCTGCAGGAAGCGCGCTGGCTCATCAAGAACATCCAGCAGCGGTTCGACACGATCCTGCGCGTCGCGCAGGCGATTGTCG
AACGTCAGAAGAATTTCTTTGCGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACGGGCAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCGCACGTCTCGACCGACACCGGTGGCGCCGCATCGTCGACCGCCATCCGTGCCCTCATCAAGCAACTGATAG
GAGCCGAAGACCCCAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAAGGCTTCGTGGTCGCGCGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAGGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.775

100

0.695

Multiple sequence alignment