Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   DM82_RS18915 Genome accession   NZ_CP008726
Coordinates   4184193..4185701 (-) Length   502 a.a.
NCBI ID   WP_010101606.1    Uniprot ID   A0AAI8B3F6
Organism   Burkholderia oklahomensis strain EO147     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4179193..4190701
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DM82_RS18890 (DM82_3743) - 4180212..4180844 (-) 633 WP_010101591.1 LON peptidase substrate-binding domain-containing protein -
  DM82_RS18895 (DM82_3744) rapZ 4180871..4181764 (-) 894 WP_010101593.1 RNase adapter RapZ -
  DM82_RS18900 (DM82_3745) hprK 4181839..4182807 (-) 969 WP_004195225.1 HPr(Ser) kinase/phosphatase -
  DM82_RS18905 (DM82_3746) ptsN 4182936..4183445 (-) 510 WP_025989646.1 PTS IIA-like nitrogen regulatory protein PtsN -
  DM82_RS18910 (DM82_3747) hpf 4183681..4184040 (-) 360 WP_010101605.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  DM82_RS18915 (DM82_3748) rpoN/rpoN1 4184193..4185701 (-) 1509 WP_010101606.1 RNA polymerase factor sigma-54 Machinery gene
  DM82_RS18920 (DM82_3749) lptB 4185869..4186645 (-) 777 WP_010101607.1 LPS export ABC transporter ATP-binding protein -
  DM82_RS18925 (DM82_3750) lptA 4186642..4187307 (-) 666 WP_010101609.1 lipopolysaccharide transport periplasmic protein LptA -
  DM82_RS18930 (DM82_3751) lptC 4187326..4187940 (-) 615 WP_010101611.1 LPS export ABC transporter periplasmic protein LptC -
  DM82_RS18935 (DM82_3752) - 4187945..4188484 (-) 540 WP_010101612.1 KdsC family phosphatase -
  DM82_RS18940 (DM82_3753) kdsD 4188484..4189467 (-) 984 WP_010101613.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 55199.40 Da        Isoelectric Point: 4.9411

>NTDB_id=123912 DM82_RS18915 WP_010101606.1 4184193..4185701(-) (rpoN/rpoN1) [Burkholderia oklahomensis strain EO147]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENEDDWIASPLRVAADGSVIAQAPASSAPEPMQ
GASNNTSATNERTERDEPAGVDEYDSYSADSGDASQWNLDDFGRSPSASDDDDLPPMQIHETTTSLRDHLAAQLRVTQAS
PRDRALVLFLIESLDDDGYLTASLEEVLADLPAELEVDLDELNAALALLHSFDPAGVGARSPSECLKLQLLRLNPSPTRA
LALDIVSQHLELLAARDFTRLRKQLKASDDELRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQSWQAELNPEVV
PRLRINRLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQRNFFAHGEIAMRPLVLREIADTL
GLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=123912 DM82_RS18915 WP_010101606.1 4184193..4185701(-) (rpoN/rpoN1) [Burkholderia oklahomensis strain EO147]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAGCATCTCGCGCTCACACCCCAGCTTCAGCAGTCGATCCGGCTGCTGCA
GCTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCGGTCGCGCAGAATCCGCTTCTCGAAAACGAAGACGACTGGA
TCGCGAGCCCGCTGCGCGTCGCGGCCGACGGCTCGGTGATCGCGCAGGCGCCCGCATCGTCTGCGCCCGAGCCGATGCAA
GGCGCATCGAACAACACGAGTGCGACGAATGAGCGCACCGAGCGCGACGAGCCTGCAGGCGTCGACGAATACGACAGCTA
TTCGGCCGACAGCGGCGACGCGAGCCAGTGGAACCTCGACGACTTCGGCCGCTCGCCCTCCGCTTCCGACGACGACGATC
TGCCGCCGATGCAGATCCACGAAACGACGACGTCGCTGCGCGACCATCTCGCCGCGCAACTGCGCGTCACGCAAGCGAGC
CCGCGCGATCGCGCGCTGGTGCTGTTCCTGATCGAATCGCTCGACGACGACGGCTATCTGACCGCGTCGCTCGAAGAAGT
GCTCGCCGATCTGCCGGCGGAACTCGAGGTCGACCTCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGATC
CTGCAGGCGTCGGCGCGCGCTCCCCATCCGAGTGCCTGAAGCTGCAGTTGCTGCGGCTGAATCCGTCCCCGACGCGCGCG
CTCGCGCTCGATATCGTGTCGCAGCATCTCGAACTGCTCGCCGCACGCGATTTCACGCGCCTGCGCAAGCAACTGAAGGC
GTCCGACGACGAGCTGCGCGACGCGCACGCGCTCATCCGCTCGCTCGAGCCTTTCCCCGGCGCGGCGTACGGCAAGGCCG
AAGCGGATTACGTGGTGCCCGACATCATCGTGAAGAAGGCGGGGCAGAGCTGGCAGGCGGAACTCAATCCGGAAGTCGTG
CCGCGGCTGCGGATCAACCGTCTGTACGCGAACATCCTGCGCAACAGCCGAGGCGATCCGAGCGCGGGTTCGCTCAAGCA
GCAACTGCAGGAAGCGCGCTGGCTGATCAAGAATATTCAGCAGCGGTTCGAGACGATCTTGCGCGTCGCGCAAGCGATTG
TCGAACGTCAGAGGAATTTCTTCGCGCACGGCGAAATTGCGATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGCCTACACGAGTCGACTGTCTCCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGCACGCTTGAATTTAAGTA
CTTCTTCGGATCGCACGTTTCGACAGACACCGGAGGTGCCGCGTCGTCGACGGCAATCCGCGCCCTCATCAAACAACTGA
TAGGAGCCGAAGACCCCAAATCCCCTCTTTCGGATAGTCGCATAGCCGAACTGCTAGCGGAACAAGGGTTCGTGGTCGCG
CGCCGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCGGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-500)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.452

100

0.687

Multiple sequence alignment