Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EV46_RS02355 Genome accession   NZ_CP007744
Coordinates   525069..526451 (-) Length   460 a.a.
NCBI ID   WP_039289804.1    Uniprot ID   -
Organism   Pectobacterium atrosepticum strain JG10-08     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 520069..531451
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EV46_RS02330 (EV46_02385) pepP 520206..521531 (-) 1326 WP_039289789.1 Xaa-Pro aminopeptidase -
  EV46_RS02335 (EV46_02390) - 521637..522224 (-) 588 WP_039289793.1 YecA family protein -
  EV46_RS02340 (EV46_02395) zapA 522417..522746 (+) 330 WP_011092083.1 cell division protein ZapA -
  EV46_RS02345 (EV46_02400) - 523045..523686 (+) 642 WP_039289797.1 5-formyltetrahydrofolate cyclo-ligase -
  EV46_RS02350 (EV46_02405) nadR 523683..524936 (-) 1254 WP_039289800.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  EV46_RS02355 (EV46_02410) radA 525069..526451 (-) 1383 WP_039289804.1 DNA repair protein RadA Machinery gene
  EV46_RS02360 (EV46_02415) serB 526469..527446 (-) 978 WP_039289807.1 phosphoserine phosphatase -
  EV46_RS02365 (EV46_02420) - 527603..528298 (+) 696 WP_011092088.1 YtjB family periplasmic protein -
  EV46_RS02370 (EV46_02425) - 528352..529254 (-) 903 WP_011092089.1 hypothetical protein -
  EV46_RS02375 (EV46_02430) prfC 529546..531135 (+) 1590 WP_011092090.1 peptide chain release factor 3 -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49615.25 Da        Isoelectric Point: 7.4463

>NTDB_id=123032 EV46_RS02355 WP_039289804.1 525069..526451(-) (radA) [Pectobacterium atrosepticum strain JG10-08]
MAKAIKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLASASVSRSDRLTGYAGESAGVSRVQKLSEISLEALPRFSTG
FLEFDRVLGGGVVPGSAILIGGSPGAGKSTLLLQTLCKLSEHMKTLYVTGEESLQQVAMRAHRLNLPTQNLNMLSETSIE
QICLIAEQEQPKLMVIDSIQVMHLTDIQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREISNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDQ
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALLLSLVSSFRDRPLPQDLVIFGEV
GLAGEIRPVPSGQERITEAAKHGFKRAIIPHANMPKKAPASMQVFGVKKLADALAILDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=123032 EV46_RS02355 WP_039289804.1 525069..526451(-) (radA) [Pectobacterium atrosepticum strain JG10-08]
GTGGCAAAAGCCATCAAACGGGCGTTTGTATGTAATGAATGCGGAGCTGACTATCCGCGCTGGCAAGGGCAGTGTAGTGC
CTGCCACGCCTGGAACACCATTACCGAAGTTCGCCTGGCGTCGGCGTCCGTATCACGCTCCGACCGTCTCACTGGCTATG
CGGGTGAGAGCGCAGGTGTCAGCCGGGTGCAAAAGCTCTCAGAAATCAGCCTTGAAGCGTTACCTCGCTTTTCCACTGGC
TTTCTGGAGTTTGATCGCGTTCTGGGCGGCGGCGTCGTTCCAGGCAGCGCCATTCTAATCGGCGGTAGCCCCGGCGCGGG
TAAGAGTACGCTGTTACTGCAAACGCTCTGCAAGCTGTCAGAACATATGAAAACCCTGTACGTCACTGGGGAAGAATCAT
TGCAGCAGGTGGCGATGCGAGCGCACCGCCTCAATCTACCAACTCAGAATCTCAACATGCTGTCGGAAACCAGCATCGAA
CAGATTTGCCTGATTGCCGAGCAAGAACAGCCGAAGTTGATGGTGATAGACTCCATTCAGGTCATGCATCTCACCGACAT
TCAATCGTCTCCCGGCAGCGTGGCGCAAGTGCGAGAAACCGCCGCCTACCTGACGCGCTTCGCTAAAACGCGCGGCGTCG
CCATTGTGATGGTCGGCCACGTCACCAAAGACGGCTCACTCGCCGGGCCGAAAGTATTGGAACACTGTATCGACTGTTCC
GTACTGCTGGACGGCGATGCAGACTCGCGCTTTCGCACGCTGCGCAGCCATAAAAACCGTTTCGGTGCCGTTAACGAGCT
AGGCGTGTTCGCCATGACGGAACAAGGGCTGCGTGAGATCAGCAATCCGTCAGCCATTTTCCTCAGCCGTGGTGACGAAG
TGACGTCTGGCAGTTCAGTCATGGTGGTATGGGAAGGTACGCGTCCGCTGCTGGTCGAGATTCAGGCGCTGGTAGATCAA
TCGATGATGGCCAACCCGCGTCGCGTTGCGGTCGGGCTGGAGCAAAACCGATTAGCGATTCTGCTGGCGGTACTGCATCG
CCACGGTGGCTTACAAATGTCGGATCAGGATGTGTTCGTGAATGTCGTCGGCGGCGTCAAAGTGACCGAAACCAGCGCCG
ATCTTGCGCTTCTGCTATCGCTGGTTTCCAGCTTCCGCGATCGCCCGCTGCCACAGGATCTGGTCATCTTCGGTGAAGTC
GGTCTGGCAGGCGAAATTCGTCCGGTTCCCAGCGGCCAAGAACGGATTACCGAAGCCGCCAAGCACGGCTTCAAACGCGC
CATCATTCCTCATGCCAATATGCCGAAGAAAGCTCCAGCCAGTATGCAGGTATTCGGCGTGAAAAAACTGGCCGACGCGC
TGGCGATCCTCGACGACCTCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(89-225)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.478

100

0.485
  radA Streptococcus mitis NCTC 12261

42.763

99.13

0.424
  radA Streptococcus pneumoniae Rx1

42.763

99.13

0.424
  radA Streptococcus pneumoniae D39

42.763

99.13

0.424
  radA Streptococcus pneumoniae R6

42.763

99.13

0.424
  radA Streptococcus pneumoniae TIGR4

42.763

99.13

0.424
  radA Streptococcus mitis SK321

42.763

99.13

0.424

Multiple sequence alignment