Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   BMMGA3_RS04480 Genome accession   NZ_CP007739
Coordinates   902302..904119 (+) Length   605 a.a.
NCBI ID   WP_004433617.1    Uniprot ID   I3E7R1
Organism   Bacillus methanolicus MGA3     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 897302..909119
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BMMGA3_RS04460 (BMMGA3_04545) spxA 897892..898287 (+) 396 WP_004433609.1 transcriptional regulator SpxA -
  BMMGA3_RS04465 (BMMGA3_04550) mecA 898615..899283 (+) 669 WP_004433611.1 adaptor protein MecA Regulator
  BMMGA3_RS04470 (BMMGA3_04555) cls 899368..900879 (+) 1512 WP_004433613.1 cardiolipin synthase -
  BMMGA3_RS04475 (BMMGA3_04560) - 900979..902175 (+) 1197 WP_004433615.1 competence protein CoiA -
  BMMGA3_RS04480 (BMMGA3_04565) pepF 902302..904119 (+) 1818 WP_004433617.1 oligoendopeptidase F Regulator
  BMMGA3_RS04490 (BMMGA3_04570) - 904707..905591 (-) 885 WP_004433620.1 ClpXP adapter SpxH family protein -
  BMMGA3_RS04495 (BMMGA3_04575) - 905588..905994 (-) 407 Protein_853 globin -
  BMMGA3_RS04500 (BMMGA3_04585) - 906227..906856 (-) 630 WP_004433624.1 lytic transglycosylase domain-containing protein -
  BMMGA3_RS04505 (BMMGA3_04590) - 906879..907457 (-) 579 WP_004433626.1 CYTH domain-containing protein -
  BMMGA3_RS04510 (BMMGA3_04595) - 907594..907953 (+) 360 WP_004433628.1 hypothetical protein -
  BMMGA3_RS04515 (BMMGA3_04600) - 908022..908657 (+) 636 WP_004433630.1 GTP pyrophosphokinase family protein -

Sequence

Protein


Download         Length: 605 a.a.        Molecular weight: 69989.95 Da        Isoelectric Point: 5.1656

>NTDB_id=122991 BMMGA3_RS04480 WP_004433617.1 902302..904119(+) (pepF) [Bacillus methanolicus MGA3]
MSNNKAVKKLPARNEIPVEETWRLEDIFATDDEWEKEFEEVKKLIPGINRYKGKLGENADELYNALTFQDQLLERLGKLY
TYAHMRYDQDTTNSFYQGMDDRIKNLYSQAASALAFIVPEILSIEEQKVAAFLEEKEELRLYKHALEEINLQRPHVLSAE
QEALLAEAAEVMNASSNTFGMLNNADLVFPSIKDENGEEVEVTHGRYIRFLESSDRRVRKDAFMAVYSTYGKYRNTFAST
LSGTVKKDNFNARVRKYNSARHAALAANNIPESVYDNLVNTINDNLHLLHRYVKLRKKVLGLDKLHMYDLYTPLVKDVKM
EITYEEAKEIIVKGLAPLGEDYVNVLKEGFENRWVDVRENKGKRSGAYSSGAYGTNPYILMNWQDNVNNLFTLAHEFGHS
VHSYFTRKNQPYPYGNYSIFVAEVASTCNEALLNDYLLKTIDDEKKRLYLLNHYLEGFRGTVFRQTMFAEFEHLIHQKAQ
NHEPLTADTLTKEYYELNKKYFGLEDIEIDEEIGLEWARIPHFYYNYYVYQYATGFSAATALSKQILEEGEPAVSRYIEF
LKAGSSDYPIEVLKKAGVDMTSPKPIEEACKVFEEKLNEMESLLS

Nucleotide


Download         Length: 1818 bp        

>NTDB_id=122991 BMMGA3_RS04480 WP_004433617.1 902302..904119(+) (pepF) [Bacillus methanolicus MGA3]
ATGTCAAATAATAAAGCAGTGAAAAAATTGCCAGCTAGAAATGAAATTCCAGTTGAAGAAACTTGGAGGCTAGAGGATAT
TTTTGCAACTGATGATGAATGGGAAAAAGAATTTGAAGAAGTAAAGAAGTTAATTCCTGGTATTAATCGTTATAAAGGGA
AGCTCGGAGAAAATGCAGATGAGTTATATAATGCGCTAACATTTCAGGACCAACTATTAGAACGCCTTGGAAAACTGTAT
ACATACGCGCATATGCGTTATGACCAAGATACAACCAATTCTTTTTACCAAGGAATGGATGACAGAATAAAAAACCTTTA
TTCACAAGCAGCGAGCGCTCTTGCTTTTATTGTTCCTGAAATATTGTCAATTGAAGAACAAAAGGTTGCCGCCTTCTTAG
AAGAAAAAGAAGAATTGAGGTTGTATAAACACGCATTAGAAGAAATCAATTTACAAAGGCCTCATGTCCTTTCTGCCGAG
CAGGAGGCACTTTTGGCAGAAGCAGCTGAGGTCATGAATGCATCAAGCAATACATTCGGAATGTTAAATAATGCAGATCT
TGTGTTTCCGAGCATTAAAGATGAGAACGGAGAAGAAGTAGAAGTAACTCATGGACGGTACATCCGATTCCTTGAAAGCA
GCGACCGCAGGGTTCGAAAAGATGCATTTATGGCAGTTTACAGCACTTATGGGAAGTACCGGAATACATTTGCCAGCACT
TTAAGCGGTACGGTGAAAAAGGATAATTTTAACGCACGAGTTCGTAAATACAACTCGGCCCGCCATGCTGCATTGGCAGC
AAATAACATTCCAGAAAGTGTATACGATAATCTTGTAAATACAATAAATGATAATCTTCATTTATTGCACCGTTACGTCA
AGCTGCGAAAAAAAGTTCTTGGTTTAGATAAACTTCATATGTATGATTTATACACTCCACTTGTTAAAGACGTGAAAATG
GAAATTACTTATGAAGAGGCAAAGGAGATCATTGTAAAGGGCCTGGCCCCGTTGGGAGAAGATTACGTAAATGTGCTAAA
AGAAGGTTTTGAGAACCGCTGGGTTGACGTACGTGAAAATAAGGGCAAGCGAAGCGGGGCCTACTCTTCAGGAGCATATG
GAACAAATCCATATATCTTAATGAATTGGCAGGACAATGTAAATAATTTATTTACACTAGCCCACGAATTTGGCCATTCT
GTTCACAGTTACTTTACACGCAAAAATCAGCCTTACCCTTATGGCAACTATTCAATATTTGTCGCTGAGGTTGCTTCAAC
ATGCAATGAAGCACTGTTGAATGATTATCTTTTAAAAACAATTGATGATGAGAAAAAACGTCTTTATTTGCTGAATCATT
ATTTGGAAGGGTTCAGAGGAACGGTGTTTCGCCAAACGATGTTTGCAGAATTTGAACATCTCATTCATCAAAAAGCACAA
AATCATGAGCCATTGACAGCTGATACGTTAACGAAAGAATATTATGAACTAAATAAGAAATATTTTGGTTTGGAAGATAT
TGAAATTGATGAAGAAATTGGACTCGAGTGGGCCAGAATACCTCATTTTTATTACAATTATTATGTGTACCAATATGCAA
CCGGCTTCAGTGCTGCAACAGCATTAAGCAAACAAATTCTTGAAGAAGGCGAGCCGGCGGTAAGCCGTTATATTGAATTT
TTAAAAGCTGGCAGTTCCGATTATCCGATTGAGGTGCTCAAAAAAGCAGGAGTTGATATGACGAGTCCAAAACCGATTGA
AGAAGCATGTAAAGTTTTTGAAGAAAAATTAAATGAAATGGAAAGTTTGCTTTCATAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(211-591)

Oligopeptidase F (Peptidase_M3_N)

(120-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I3E7R1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

51.52

97.851

0.504

Multiple sequence alignment