Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   EP20_RS00200 Genome accession   NZ_CP007657
Coordinates   46509..48137 (-) Length   542 a.a.
NCBI ID   WP_001186604.1    Uniprot ID   Q8VUV3
Organism   Staphylococcus aureus strain V2200     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 39744..55115 46509..48137 within 0


Gene organization within MGE regions


Location: 39744..55115
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EP20_RS00165 (EP20_000165) mecA 39744..41753 (-) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  EP20_RS00170 (EP20_000170) - 41850..42836 (+) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  EP20_RS00175 (EP20_000175) - 42808..43068 (+) 261 WP_001549961.1 type I restriction enzyme endonuclease domain-containing protein -
  EP20_RS00180 (EP20_000180) - 43059..44666 (-) 1608 Protein_33 IS1182 family transposase -
  EP20_RS00185 (EP20_000185) - 44718..45224 (-) 507 WP_001092189.1 DUF1643 domain-containing protein -
  EP20_RS00190 (EP20_000190) - 45239..45550 (-) 312 WP_000700851.1 DUF960 family protein -
  EP20_RS00195 (EP20_000200) - 45637..45987 (-) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  EP20_RS00200 (EP20_000205) ccrB 46509..48137 (-) 1629 WP_001186604.1 recombinase family protein Machinery gene
  EP20_RS00205 (EP20_000210) ccrA 48159..49508 (-) 1350 WP_000815652.1 recombinase family protein Machinery gene
  EP20_RS00210 (EP20_000215) - 49742..51529 (-) 1788 WP_000122621.1 DUF927 domain-containing protein -
  EP20_RS00215 (EP20_000220) - 51529..51819 (-) 291 WP_001036437.1 hypothetical protein -
  EP20_RS00220 (EP20_000225) - 51958..53007 (+) 1050 WP_000613248.1 hypothetical protein -
  EP20_RS00225 (EP20_000230) - 53625..55115 (+) 1491 WP_000554504.1 RNA-binding domain-containing protein -

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 62650.85 Da        Isoelectric Point: 9.9805

>NTDB_id=122037 EP20_RS00200 WP_001186604.1 46509..48137(-) (ccrB) [Staphylococcus aureus strain V2200]
MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFEVKDIYADRGISGKSMNRPELQRMLKDAKEGNIDCVM
IYKTNRLARNTSDLLKIVEDLHKQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
LPLGYDKIPDSKHELMINQHEANIVKYIFESYAKGHGYRKIANALNHKGYVTKKGKPFSISSITYILANPFYIGKIQFAK
YKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQMRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKD
GTKKRIRYYSCSNFRNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVARVNQENKVDGAALHHDIAYKQQQYD
EVQTKLNNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNEDKSLFDAKQISKLLQHIFQDIKHMEKSRLK
ALYLSVIDRINIRKDGNHKKQFYVTLKLNNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=122037 EP20_RS00200 WP_001186604.1 46509..48137(-) (ccrB) [Staphylococcus aureus strain V2200]
ATGCAACAACTTAAAACAAAACGTGTCGGTATCTATGTACGTGTATCAACAGAAATGCAAAGCACAGAAGGTTATAGTAT
CGACGGACAAATCAATCAAATCAAAGAATACTGTGACTTCCATCATTTTGAAGTTAAAGATATATACGCTGATCGTGGTA
TTTCAGGTAAATCTATGAACAGACCTGAGCTCCAACGTATGTTGAAAGATGCAAAAGAAGGCAATATAGATTGTGTTATG
ATCTACAAAACAAACCGATTAGCTCGTAATACATCGGATCTTCTGAAAATCGTCGAAGATTTGCATAAACAAAATGTCGA
ATTTTTCAGTTTATCAGAGCGTATGGAAGTCAATACTTCTTCTGGTAAACTCATGTTACAAATACTTGCGAGTTTCTCAG
AATTCGAACGTAATAACATTGTCGAGAATGTATTTATGGGGCAAACGAGACGTGCCCAAGAAGGCTATTATCAAGGCAAT
TTACCACTAGGTTATGACAAAATACCAGATAGTAAACACGAGCTAATGATTAACCAACATGAAGCTAATATTGTAAAATA
TATATTCGAGTCCTATGCCAAAGGACATGGCTATCGTAAAATAGCCAATGCATTGAATCACAAAGGCTATGTCACTAAAA
AGGGTAAACCTTTTAGTATTAGTTCTATCACATATATATTAGCTAACCCATTCTATATCGGCAAAATTCAATTTGCGAAA
TACAAAGATTGGAGTGAAAAACGTCGTAAAGGGCTGAATGATAAACCAGTGATAGCTGAAGGTAAGCATTCCCCCATTAT
TAATCAAGATTTATGGGATAAAGTACAAATGCGTAAAAAACAAGTCAGTCAAAAACCCCAAGTTCATGGTAAAGGAACGA
ATCTGCTTACAGGCATTATCCATTGTCCCCAATGTGGCGCACCTATGGCAGCAAGCAATACAACGAATACACTTAAAGAT
GGGACCAAGAAACGTATTCGTTACTATTCATGTAGTAATTTTCGGAACAAGGGTTCCAAAGTATGTTCGGCAAACAGTGT
AAGAGCTGATGTGATTGAAGATTATGTAATGAAGCAAATACTCGAAATAGTCAAAAGTGATAAAGTCATTCAACGCGTTG
TAGCACGTGTTAATCAAGAAAATAAAGTTGATGGCGCTGCACTTCATCACGATATTGCTTATAAGCAACAACAATATGAT
GAAGTACAAACCAAACTAAATAACTTGATTAAAACCATAGAGGATAATCCGGACTTAACATCAGTAATCAGACCAAGTAT
TCAAAAATATGAAAAGCAACTCAATGACATTACGAATCAAATCAACCAACTCAAAAATCAACAAAATGAAGATAAGTCTT
TATTTGATGCCAAACAAATCAGTAAACTATTACAACACATCTTTCAAGATATTAAGCACATGGAAAAATCTCGACTCAAA
GCATTGTACCTATCAGTGATTGATCGTATTAATATTAGAAAAGATGGTAATCATAAAAAACAATTCTATGTCACACTCAA
ACTTAATAACGAAATCATTAAACAACTTTTCAATAATAAACAACTCGACGAAGTGCATCTCAGCACTTCGTCTTTATTTT
TGCCCCAAACACTATATCTTACTATTTAG

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(11-156)

Recombinase (Recombinase)

(180-280)

Ogr/Delta-like zinc finger (Ogr_Delta)

(301-356)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8VUV3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

97.417

100

0.974

Multiple sequence alignment