Detailed information NTG0121667

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: DF16_RS24600
• Genome accession: NZ_CP007607
• Coordinates: 4697535..4701260 (-)
• Length: 1241 a.a.
• NCBI ID: WP_000572259.1
• Uniprot ID: -
• Organism: Bacillus thuringiensis serovar kurstaki str. YBT-1520
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 4692535..4706260

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DF16_RS24550	-	4693370..4693654 (+)	285	WP_000926864.1	hypothetical protein	-
DF16_RS24555 (DF16_orf04949)	-	4693690..4694589 (-)	900	WP_001213086.1	fumarylacetoacetate hydrolase family protein	-
DF16_RS24560 (DF16_orf04950)	-	4694836..4695015 (+)	180	WP_000462845.1	aspartyl-phosphate phosphatase Spo0E family protein	-
DF16_RS24565 (DF16_orf04951)	gerPA	4695112..4695333 (+)	222	WP_001111187.1	spore germination protein GerPA	-
DF16_RS24570 (DF16_orf04952)	gerPB	4695348..4695554 (+)	207	WP_001012508.1	spore germination protein GerPB	-
DF16_RS24575 (DF16_orf04953)	gerPC	4695622..4696236 (+)	615	WP_038415551.1	spore germination protein GerPC	-
DF16_RS24580 (DF16_orf04954)	gerPD	4696243..4696437 (+)	195	WP_001052807.1	spore germination protein GerPD	-
DF16_RS24585 (DF16_orf04955)	-	4696453..4696839 (+)	387	WP_000902319.1	spore germination protein GerPE	-
DF16_RS24590 (DF16_orf04956)	gerPF	4696882..4697097 (+)	216	WP_001141566.1	spore germination protein GerPF	-
DF16_RS24595 (DF16_orf04957)	-	4697235..4697522 (-)	288	WP_000255727.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
DF16_RS24600 (DF16_orf04958)	addA	4697535..4701260 (-)	3726	WP_000572259.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
DF16_RS24605 (DF16_orf04959)	addB	4701257..4704772 (-)	3516	WP_000058598.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
DF16_RS24610 (DF16_orf04960)	lepB	4704889..4705452 (-)	564	WP_000751910.1	signal peptidase I	-
DF16_RS24615 (DF16_orf04961)	-	4705509..4706102 (-)	594	WP_000347518.1	TVP38/TMEM64 family protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142591.58 Da
• Isoelectric Point: 4.7416

>NTDB_id=121667 DF16_RS24600 WP_000572259.1 4697535..4701260(-) (addA) [Bacillus thuringiensis serovar kurstaki str. YBT-1520]
MIENWPEKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRIETLQADVALLGTLSSAAQESWTSLYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSENGEMNPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGATYPEGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKNDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLSDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLESERKDEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEDGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITVEVLQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGNSGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVNIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=121667 DF16_RS24600 WP_000572259.1 4697535..4701260(-) (addA) [Bacillus thuringiensis serovar kurstaki str. YBT-1520]
ATGATAGAAAATTGGCCTGAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCTGTTGTAGCGAATGGACG
TGATATTTTAGTTGCGGCTGCAGCTGGGTCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATTAATG
AAGAGAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACCGAATC
GGGGAAGCGTTAGAAAAGGTATTAATTGATGAACCAGGCTCTCAACACGTAAGAAAGCAGCTGAGCCTATTAAATAAAGC
TTCCATTTCAACAATTCACTCATTCTGTTTACAAGTAATTAGAGGATATTACTACATGCTTGATGTTGATCCTCGTTTCC
GTATTGCGAATCAAACAGAAAATGAATTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTACGGAATCGAA
GATAATACAATCTTCTTTGAACTTGTTGATCGTTATACGAGTGACCGTAGTGATGATGACTTACAACGTATGATTTTAGC
GCTTCATACAGAGTCAAGAGCGCATCCAAATCCGGAAAAATGGCTTGATAAATTAGTAGAAGCATATGACGTAGAAGGAA
AGACAATTGAAGATTTAGTATATGCTTCTTATTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAGGCAACTGAACTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCATTGAAACCCTGCAAGCAGATGTAGCTTTACT
TGGAACGCTATCATCAGCTGCTCAGGAGTCGTGGACAAGTTTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAATGAAGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGTTATTTAGCCGAAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCACCCAGTATTAGA
AAAGCTTGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAAATGGTGAAATGAATCCGTCAGCAGTGGCGCTCCAA
TATCGTAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGATACGAACTTTGTACAGGAATCTATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTTGGTGACGTAAAGCAGTCGATTTATCGTTTCCGACTAGCCG
AACCAGGACTATTCCTAGGAAAATATAAACGCTTCACACAAGAAGGATTGGGCGGTGGAATGAAGATCGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCAGGTACGAACTTTATCTTCAAACAAATTATGGGTGAAGAAGTCGGAGAAAT
CGACTACGATGCAGACGCTGAATTAAAGTTAGGTGCTACCTATCCAGAAGGTGAAGATGTAGCGGCAGAACTACTGTGCA
TTCAGCAAACGGAAGAAGAAGTAATAGACGGAGAAGAAGGTGCAGAAGTCGAAAAAGCACAGCTTGAAGCTCGTCTTATG
GCGCAGCGCATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGACCGTAAAAATGATAGTATGCGCCCAGTACAATA
CCGCGACTTCGTTATTTTACTTCGCTCCATGCCGTGGGCCCCGCAAATTATGGAAGAGTTAAAATTACAAGGAATTCCAG
TGTACGCTGACCTTGCGACTGGTTACTTTGAAGCGACGGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCGCTTGCAGCTGTACTTCGTTCTCCAATCGTTGGATTGAGCGATGAAGAACTTGCAACGCTTCG
TGCTCATGGAAAGAAAGGCTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGGGCACCACTTGAAGAGGAGCAAGAAC
TTCATGATAAATTAGAGTGGTTTTATAACTTACTGCAAGGATGGCGTGAATTTGCACGTCAACAATCACTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGACTTCGTTGGCGGTTTACCAGCTGGAAAGCAAAGGCAGGCAAACTT
ACGCGTACTATATGACCGCGCAAGACAATACGAAGCAACATCATTTAGAGGATTATTCCGCTTCTTACGTTTTATTGAAC
GTATTTTAGAACGCGGTGATGACATGGGTACGGCGAGGGCCCTCGGTGAACAAGAAGACGTTGTTCGCATTATGACGATT
CATAAAAGTAAAGGGTTAGAGTTCCCGGTTGTATTTGTAGCTGGGCTCGGTCGTCGTTTTAATACACAAGACTTAATGAA
ACGTTTCTTATTGCATAAAGATTTCGGTTTCGGTTCGCAATTTATCGATCCGCGTAAACGAATTAAATATACAACATTAT
CACAGCTTGCGATTAAGCGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTACTATACGTAGCGTTGACGCGC
GCGAAAGAGAAGTTAATTTTAATCGGAACAGTTAAGGATGCAAATAAGGAAATGGAAAAATGGCTTGATGCGAGGGAACA
TAGTGAATGGTTATTACCAGATCACATACGTGCCGGGGCGTCTTGTTATTTAGACTGGATTGCCCCTTCATTATATAGAC
ACCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAGTATTCCAGATGAAATTTACGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTGGACGGTAATACGCTACTTGCACCAGAGCCAGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCGCTTCG
TGAGAAAAAGGCTGTGCCGTTAGAAAGTGAACGAAAAGATGAAGTGTACGACAGATTAATGTGGAAGTACGGATATGAGG
AAGCAACATCTCACCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCAGAAGATGGTAGTGATAACGCC
TTTATTAAAAAACTACGTGCACCAATTAAAACACGTCCGCGCTTTATGGAGAAAAAAGGGTTAACGTACGCAGAGCGCGG
AACAGCAGTCCATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACGGTTGAAGTTCTTCAAGAGCAAATTGCTG
GAATGGTAAATAAGGAATTATTAACATTCGAGCAGGCGGAAGAAATAGCGATTGAAAAAGTAATTTCATTCTTTGACAGT
GACTTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTTCCATTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGGAATAGCGGGGAATCGATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GCATTACGTTAATCGACTTCAAAACAGATACGATTGAAGGGAAATTTCCAGGCGGATTTGAACAAGCGAAACCAATTTTA
GAAGATCGATATAAAGTGCAGCTTTCGTTATATGCAAAAGCACTGGAGAAAAGCTTACAACATCCTGTAAAAGAGAAATG
TTTATACTTCTTTGATGGGAATCACGTTGTAAATATTGAAGAATAG

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1231)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.488	100	0.537