Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DF16_RS01440 Genome accession   NZ_CP007607
Coordinates   238160..239536 (-) Length   458 a.a.
NCBI ID   WP_001085202.1    Uniprot ID   -
Organism   Bacillus thuringiensis serovar kurstaki str. YBT-1520     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 233160..244536
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DF16_RS01420 (DF16_orf00244) ispF 234523..234999 (-) 477 WP_000488391.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  DF16_RS01425 (DF16_orf00245) ispD 235116..235796 (-) 681 WP_000288282.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  DF16_RS01430 (DF16_orf00246) - 235813..236922 (-) 1110 WP_000919677.1 PIN/TRAM domain-containing protein -
  DF16_RS01435 (DF16_orf00247) disA 237083..238156 (-) 1074 WP_000392158.1 DNA integrity scanning diadenylate cyclase DisA -
  DF16_RS01440 (DF16_orf00248) radA 238160..239536 (-) 1377 WP_001085202.1 DNA repair protein RadA Machinery gene
  DF16_RS01445 (DF16_orf00249) clpC 239632..242067 (-) 2436 WP_000971187.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  DF16_RS01450 (DF16_orf00250) - 242090..243154 (-) 1065 WP_000050842.1 protein arginine kinase -
  DF16_RS01455 (DF16_orf00251) - 243159..243707 (-) 549 WP_000128398.1 UvrB/UvrC motif-containing protein -
  DF16_RS01460 (DF16_orf00252) ctsR 243878..244339 (-) 462 WP_001244565.1 transcriptional regulator CtsR -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49844.48 Da        Isoelectric Point: 7.1422

>NTDB_id=121625 DF16_RS01440 WP_001085202.1 238160..239536(-) (radA) [Bacillus thuringiensis serovar kurstaki str. YBT-1520]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNSLVEEMEPVVSSRRLNYANAIQTEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADASYDVLYISGEESAKQIKLRADRLHVNGSNLFVVSETDLQRIA
AHIEEMNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPEGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=121625 DF16_RS01440 WP_001085202.1 238160..239536(-) (radA) [Bacillus thuringiensis serovar kurstaki str. YBT-1520]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGTTATCAATCACCAAAATATATGGGTAAATGTCCAGGGTG
TGGTCAATGGAATTCGCTTGTTGAAGAGATGGAACCCGTTGTATCATCCAGACGCCTAAATTATGCAAATGCAATTCAAA
CAGAAGTAACAAAACCAAGACGTCTTACAGAAGTAGAAACAAAGTCTGAGGCACGTATTGAAACAAAATTTCAGGAGTTT
AACCGTGTACTAGGTGGCGGAATTGTAGATGGATCTTTAGTACTTATTGGTGGGGACCCTGGGATTGGAAAATCAACGTT
ATTATTACAGATTTCCTCGCAATTAGCAGATGCTTCATATGATGTATTGTATATATCAGGTGAGGAGTCAGCAAAGCAGA
TTAAACTTCGTGCAGATCGTTTACATGTAAATGGTAGTAATCTATTTGTTGTATCAGAGACAGATTTACAGCGTATTGCA
GCACATATTGAAGAGATGAATCCTGCTTTTGTTGTTATTGATTCTATTCAAACGATACATTTACCTGAGGTGACATCAGC
TCCTGGTAGCGTGGCGCAAGTACGTGAATGTACAGCAGAATTAATGAAACTTGCAAAAACAAAGGGAATCCCAATTTTCA
TCGTAGGACATGTGACAAAAGAAGGTGCAATTGCGGGGCCGCGTATGTTAGAACATATGGTCGATGCAGTTCTTTACTTT
GAAGGAGATCGACACCATACATATCGTATTTTACGAGCTGTAAAGAATCGTTTTGGTTCTACGAATGAAATGGGTATCTT
TGAAATGAAAGAACTTGGCCTTGCAGAAGTCTTAAATCCTTCTGAAATTTTCCTTGAGGAAAGACCAGTTGGAGTTGCAG
GTTCAACAGTAGTTGCCTCAATGGAAGGAACAAGACCAGTTTTAGTAGAAATACAGGCATTAATTTCCCCTACTAGTTTT
GGAAATCCTCGAAGAATGGCGACAGGAATTGATCATAATCGTGTCTCGCTTATTATGGCGGTACTAGAGAAAAGAACAGG
TTTACTATTACAAAATCAAGATGCGTATTTAAAAGTAGCTGGTGGTTTGAAATTAGATGAACCGGCAATTGATTTAGCTG
TTGCCTTAAGTATAGCTTCAAGTTTTAGAGATAAATCTACGGCACCAACTGATGCGGTAATAGGAGAAGTTGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATCGAACAACGTGTACAAGAAGCAGCTAAATTAGGATTTCAACGTGCTATCATTCC
TAGAAAAAATTTAGGGGGGTGGACAATTCCAGAGGGGATTGAGGTTGTAGGTGTTTCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

AAA domain (AAA_25)

(60-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.728

100

0.749
  radA Streptococcus pneumoniae Rx1

64.018

98.908

0.633
  radA Streptococcus pneumoniae D39

64.018

98.908

0.633
  radA Streptococcus pneumoniae R6

64.018

98.908

0.633
  radA Streptococcus pneumoniae TIGR4

64.018

98.908

0.633
  radA Streptococcus mitis SK321

64.018

98.908

0.633
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631

Multiple sequence alignment