Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DK42_RS00840 Genome accession   NZ_CP007571
Coordinates   126685..128049 (+) Length   454 a.a.
NCBI ID   WP_001085195.1    Uniprot ID   A0AAV3JMW2
Organism   Streptococcus agalactiae strain GBS2-NM     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 121685..133049
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DK42_RS00815 (DK42_00820) rpoE 122377..122970 (+) 594 WP_041330178.1 DNA-directed RNA polymerase subunit delta -
  DK42_RS00820 (DK42_00825) - 123243..124847 (+) 1605 WP_000170433.1 CTP synthase -
  DK42_RS00825 (DK42_00830) - 124956..125882 (+) 927 WP_000723857.1 alpha/beta hydrolase -
  DK42_RS00835 (DK42_00840) - 126077..126523 (+) 447 WP_000049183.1 dUTP diphosphatase -
  DK42_RS00840 (DK42_00845) radA 126685..128049 (+) 1365 WP_001085195.1 DNA repair protein RadA Machinery gene
  DK42_RS00845 (DK42_00850) - 128185..128682 (+) 498 WP_000214743.1 carbonic anhydrase -
  DK42_RS00850 (DK42_00855) - 128836..130155 (+) 1320 WP_000759708.1 FAD-containing oxidoreductase -
  DK42_RS00855 (DK42_00860) gltX 130364..131818 (+) 1455 WP_001284501.1 glutamate--tRNA ligase -
  DK42_RS00860 (DK42_00865) - 131858..132826 (-) 969 WP_000668258.1 substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49562.84 Da        Isoelectric Point: 6.5148

>NTDB_id=121081 DK42_RS00840 WP_001085195.1 126685..128049(+) (radA) [Streptococcus agalactiae strain GBS2-NM]
MAKKKSVFTCQECGYQSPKYLGRCPNCSAWSSFVEEVEVQEVKNARVSLNGEKSRPTKLKDVSSINYSRTKTDMDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQSIRSEIE
KIKPDFLIIDSIQTIMSPEVSSVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSAIVVTMEGTRPILAEVQALVTPTVFGNAK
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTNPQESFIGEIGLTGEIR
RVTRIEQRINEASKLGFTKIYAPKNSLAGIEIPKGIDVIGVTTVSQVLKAVFST

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=121081 DK42_RS00840 WP_001085195.1 126685..128049(+) (radA) [Streptococcus agalactiae strain GBS2-NM]
GTGGCTAAGAAAAAATCAGTCTTCACCTGTCAAGAGTGTGGTTACCAATCACCTAAGTATTTGGGACGTTGTCCAAATTG
CTCAGCCTGGTCTTCATTTGTAGAAGAGGTTGAAGTTCAAGAAGTTAAAAATGCACGAGTTAGTTTGAACGGTGAAAAGT
CCCGACCGACGAAGTTAAAAGATGTTTCCTCAATTAACTATTCTAGAACTAAGACTGATATGGATGAATTTAACCGTGTT
CTAGGAGGCGGTGTTGTACCAGGAAGTTTGGTTCTGATTGGAGGAGACCCTGGGATAGGTAAATCTACTTTATTACTACA
AGTATCAACTCAGCTAGCTAATAAAGGTACAGTATTATATGTCTCAGGTGAGGAATCTGCAGAACAGATTAAGTTACGAA
GTGAGCGTCTAGGCGATATCGATAATGAATTTTACCTTTATGCCGAGACTAATATGCAAAGTATTCGGTCGGAGATTGAG
AAGATTAAACCAGATTTTCTTATTATTGATTCTATACAAACTATTATGAGTCCCGAAGTATCAAGTGTCCAAGGCTCTGT
TAGCCAAGTTCGCGAAGTGACCGCTGAATTGATGCAGTTAGCTAAAACTAATAATATTGCTACTTTTATTGTAGGTCATG
TGACTAAGGAGGGAACATTAGCAGGCCCTCGAATGCTAGAACATATGGTGGATACGGTTCTTTATTTTGAAGGGGAGCGT
CATCACACGTTCCGTATTTTACGGGCAGTCAAAAACCGTTTTGGTTCGACCAATGAAATAGGTATCTTTGAAATGCAGTC
GGGTGGTCTAGTTGAAGTTTTGAATCCAAGTCAAGTTTTTCTTGAAGAACGTTTAGACGGTGCAACAGGATCTGCTATTG
TTGTTACTATGGAAGGAACAAGGCCTATTTTAGCAGAGGTTCAAGCTTTAGTGACTCCAACTGTTTTTGGAAATGCTAAG
CGCACAACCACAGGTCTTGACTTTAATCGTGTCAGTCTTATTATGGCAGTTCTAGAAAAACGTTGTGGTTTATTGCTTCA
AAATCAAGATGCCTATTTAAAATCAGCGGGTGGTGTTAAATTGGATGAACCAGCAATTGATTTAGCTGTTGCTGTAGCTA
TTGCTTCCAGTTACAAAGAAAAACCCACAAATCCACAAGAATCATTTATTGGAGAAATTGGACTCACTGGTGAGATTCGT
CGCGTGACACGTATTGAGCAACGTATTAATGAGGCTAGTAAATTGGGCTTTACTAAAATTTACGCACCAAAGAATTCTCT
AGCTGGGATTGAAATACCTAAAGGCATTGATGTTATTGGTGTGACAACTGTTAGTCAGGTCTTAAAAGCAGTATTTTCAA
CATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

AAA domain (AAA_25)

(68-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

88.079

99.78

0.879
  radA Streptococcus pneumoniae D39

88.079

99.78

0.879
  radA Streptococcus pneumoniae R6

88.079

99.78

0.879
  radA Streptococcus pneumoniae TIGR4

88.079

99.78

0.879
  radA Streptococcus mitis NCTC 12261

88.079

99.78

0.879
  radA Streptococcus mitis SK321

87.859

99.78

0.877
  radA Bacillus subtilis subsp. subtilis str. 168

62.914

99.78

0.628

Multiple sequence alignment