Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   SH04_RS03655 Genome accession   NZ_CP010443
Coordinates   744683..746065 (+) Length   460 a.a.
NCBI ID   WP_001029687.1    Uniprot ID   P24554
Organism   Escherichia coli K-12 strain K-12 MG1655     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 739683..751065
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SH04_RS03635 (SH04_03630) yjjJ 740540..741871 (+) 1332 WP_001293115.1 type II toxin-antitoxin system HipA family toxin YjjJ -
  SH04_RS25350 (SH04_03635) lplA 741872..742888 (-) 1017 WP_000105884.1 lipoate--protein ligase LplA -
  SH04_RS25355 (SH04_03640) ytjB 742916..743560 (-) 645 WP_000124615.1 YtjB family periplasmic protein -
  SH04_RS03650 (SH04_03645) serB 743666..744634 (+) 969 WP_001132955.1 phosphoserine phosphatase -
  SH04_RS03655 (SH04_03650) radA/sms 744683..746065 (+) 1383 WP_001029687.1 DNA repair protein RadA Machinery gene
  SH04_RS03660 (SH04_03655) nadR 746086..747318 (+) 1233 WP_000093814.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  SH04_RS25850 (SH04_03660) nadS 747352..747465 (+) 114 Protein_676 NadS family protein -
  SH04_RS03670 (SH04_03665) ettA 747626..749293 (-) 1668 WP_000046749.1 energy-dependent translational throttle protein EttA -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49472.10 Da        Isoelectric Point: 7.1936

>NTDB_id=121027 SH04_RS03655 WP_001029687.1 744683..746065(+) (radA/sms) [Escherichia coli K-12 strain K-12 MG1655]
MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPMVARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=121027 SH04_RS03655 WP_001029687.1 744683..746065(+) (radA/sms) [Escherichia coli K-12 strain K-12 MG1655]
GTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGC
CTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCT
ATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGA
TTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGG
GAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGC
TGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAA
CAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGT
ACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGG
CGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCG
GTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCT
GGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAG
TGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTCGATCAC
TCGATGATGGCGAACCCACGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTGTTGCACCG
TCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCG
ACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTC
GGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGC
GATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGC
TTAGCGTGTTCGACGACTTATAA

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(89-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P24554

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

46.827

99.348

0.465
  radA Streptococcus mitis NCTC 12261

43.86

99.13

0.435
  radA Streptococcus mitis SK321

43.86

99.13

0.435
  radA Streptococcus pneumoniae TIGR4

43.421

99.13

0.43
  radA Streptococcus pneumoniae D39

43.421

99.13

0.43
  radA Streptococcus pneumoniae Rx1

43.421

99.13

0.43
  radA Streptococcus pneumoniae R6

43.421

99.13

0.43

Multiple sequence alignment