Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   B224_RS00155 Genome accession   NZ_CP007567
Coordinates   28291..29730 (+) Length   479 a.a.
NCBI ID   WP_041204880.1    Uniprot ID   -
Organism   Aeromonas media WS     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 23291..34730
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B224_RS00125 (B224_0025) mlaF 23869..24669 (-) 801 WP_005331323.1 phospholipid ABC transporter ATP-binding protein MlaF -
  B224_RS00130 (B224_0026) - 24876..25871 (+) 996 WP_041204878.1 KpsF/GutQ family sugar-phosphate isomerase -
  B224_RS00135 (B224_0027) kdsC 25871..26425 (+) 555 WP_005331333.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  B224_RS00140 (B224_0028) lptC 26422..26982 (+) 561 WP_005331338.1 LPS export ABC transporter periplasmic protein LptC -
  B224_RS00145 (B224_0029) lptA 26969..27487 (+) 519 WP_041204879.1 lipopolysaccharide transport periplasmic protein LptA -
  B224_RS00150 (B224_0030) lptB 27500..28225 (+) 726 WP_010675872.1 LPS export ABC transporter ATP-binding protein -
  B224_RS00155 (B224_0032) rpoN/rpoN1 28291..29730 (+) 1440 WP_041204880.1 RNA polymerase factor sigma-54 Machinery gene
  B224_RS00160 (B224_0033) hpf 29751..30038 (+) 288 WP_005305957.1 ribosome hibernation promoting factor -
  B224_RS00165 (B224_0034) ptsN 30041..30487 (+) 447 WP_005331355.1 PTS IIA-like nitrogen regulatory protein PtsN -
  B224_RS00170 (B224_0035) rapZ 30528..31394 (+) 867 WP_041204881.1 RNase adapter RapZ -
  B224_RS00175 (B224_0036) npr 31415..31687 (+) 273 WP_005331359.1 PTS phosphocarrier protein NPr -
  B224_RS23885 (B224_0037) - 31706..32753 (-) 1048 Protein_31 PTS sugar transporter subunit IIA -
  B224_RS00195 (B224_0043) degS 32856..33977 (-) 1122 WP_041204883.1 outer membrane-stress sensor serine endopeptidase DegS -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53771.74 Da        Isoelectric Point: 4.4457

>NTDB_id=120999 B224_RS00155 WP_041204880.1 28291..29730(+) (rpoN/rpoN1) [Aeromonas media WS]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENEAHETSPIEPQDASQLDSSDAMAQDKMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDLDAAIALAIIDAIDESGYLTVSLED
IQAAVSSEEVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLNEAREVIREHMDLLGNRDYRTLARK
TRLKESDLKEVLDLIQQLEPRPGNGIIQSESQYVIPDVSVVKKGAKWVVELNPDSAPRIRVNEAYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=120999 B224_RS00155 WP_041204880.1 28291..29730(+) (rpoN/rpoN1) [Aeromonas media WS]
ATGAAGCCGACATTACAGTTGCGTCTCGGCCAGTCCCTGACCATGACGCCACAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGATAATAACCCCTTGCTGGAGCAGGAGGAGAATGAGG
CGCACGAAACCAGTCCGATAGAGCCCCAGGACGCCAGCCAACTCGACTCCAGTGACGCAATGGCACAGGACAAGATGCCG
GATGAACTGCCGGTGGACACCACCTGGGATGAGGTCTATTCCGCCGGCACGGCTCAGAGCGCTGGCCCCATCCATGACGG
GGAAGACAGCGTCTATCAGGGGGAAACCACCGAAAATCTGCAGGATTATCTGTTGTGGCAGATGCGACTGACGCCGTTCA
GTGATCTGGATGCCGCGATTGCCCTCGCCATCATCGATGCCATCGATGAGTCTGGCTACCTGACCGTTTCTCTCGAAGAT
ATTCAGGCTGCAGTCAGCAGTGAAGAGGTAGAAGTTGAACTGGATGAAGTGGAAGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTTGGCATCGCCGCTCGCTCGGTACAGGAGTGTCTGTTGATCCAGCTTGGCCAGTATTCGCCGGATCTGCCTT
GGCTCAATGAGGCAAGGGAGGTGATCCGCGAGCACATGGACTTGCTCGGCAACCGTGACTACCGCACCCTGGCGCGCAAG
ACCCGGCTCAAGGAGAGCGACCTCAAGGAGGTACTGGATCTCATCCAGCAGCTGGAGCCCAGACCCGGTAATGGCATCAT
CCAGAGCGAATCGCAATACGTGATCCCGGATGTTTCCGTGGTCAAGAAGGGGGCGAAGTGGGTGGTTGAACTCAACCCGG
ATTCGGCGCCGCGCATTCGTGTCAACGAGGCCTATGCGGCAATGGCACGCAATGCCCGCAGCAGCACGGATACTCAGTTC
ATTCGTTCCCATTTGCAGGAAGCCAAGTGGTTCATCAAGAGTCTGGAGAGTCGAAACGACACCCTGCTCAAGGTGGCAAG
CTGCATAGTCGAGCAGCAACAGGGTTTCTTCGAGTACGGTGAAGAGGCCATGAAGCCCATGGTACTCAACGACATCGCCG
AGGCGGTGGAGATGCACGAGTCGACGATTTCACGGGTGACGACCCAGAAGTACATGCATACGCCCCGTGGCATCTTTGAA
CTGAAGTTTTTTTTCTCCAGTCATGTCAACACGGAGGGAGGAGGAGAGTGCTCATCGACCGCAATTCGTGCCCTTATCAA
GAAGCTGGTGACGGCCGAAAACCCGGCCAAACCGCTTAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.667

100

0.497

Multiple sequence alignment