Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   SH03_RS14115 Genome accession   NZ_CP010441
Coordinates   2825550..2826977 (+) Length   475 a.a.
NCBI ID   WP_000980589.1    Uniprot ID   P04995
Organism   Escherichia coli K-12 strain K-12 MG1655     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2820550..2831977
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SH03_RS14080 (SH03_14085) yeeW 2820734..2820928 (+) 195 WP_000988600.1 DUF5983 family protein -
  SH03_RS25825 (SH03_14090) - 2820974..2821054 (+) 81 Protein_2695 hypothetical protein -
  SH03_RS24195 - 2821343..2821471 (-) 129 Protein_2696 transposase domain-containing protein -
  SH03_RS14090 (SH03_14095) - 2821543..2821725 (+) 183 WP_001016348.1 EutP/PduV family microcompartment system protein -
  SH03_RS14095 (SH03_14100) tmaR 2821826..2822155 (-) 330 WP_000450409.1 DUF496 family protein -
  SH03_RS14100 (SH03_14105) yeeA 2822327..2823385 (-) 1059 WP_001200891.1 FUSC family protein -
  SH03_RS14105 (SH03_14110) sbmC 2823583..2824056 (-) 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
  SH03_RS14110 (SH03_14115) dacD 2824175..2825341 (-) 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  SH03_RS14115 (SH03_14120) sbcB 2825550..2826977 (+) 1428 WP_000980589.1 exodeoxyribonuclease I Machinery gene
  SH03_RS14120 (SH03_14125) tsuB 2827020..2827247 (-) 228 WP_000234896.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  SH03_RS14125 (SH03_14130) tsuA 2827261..2828319 (-) 1059 WP_000492339.1 thiosulfate utilization transporter TsuA/YeeE -
  SH03_RS14130 (SH03_14135) plaP 2828498..2829856 (-) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  SH03_RS25625 yoeI 2829846..2829908 (-) 63 WP_010723108.1 membrane protein YoeI -
  SH03_RS14135 (SH03_14140) yeeY 2830123..2831052 (-) 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
  SH03_RS14140 (SH03_14145) yeeZ 2831098..2831922 (-) 825 WP_000754737.1 SDR family oxidoreductase -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54500.81 Da        Isoelectric Point: 5.4700

>NTDB_id=120895 SH03_RS14115 WP_000980589.1 2825550..2826977(+) (sbcB) [Escherichia coli K-12 strain K-12 MG1655]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNIFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLVQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=120895 SH03_RS14115 WP_000980589.1 2825550..2826977(+) (sbcB) [Escherichia coli K-12 strain K-12 MG1655]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAAC
GAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTGCACGTTTCCGGAATGTTTGGAGCATGGCGC
GGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAACTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGT
GGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTG
ACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGAT
AAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTG
GCTGGAGCACCGTCGCCAAGTCTTCACGCCAGAGTTTTTGCAGGGTTATGCTGATGAATTGCAGATGCTGGTACAACAAT
ATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCACTTTGGCAGTACGCGGAAGAGATTGTCTAA

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(13-188)

Exonuclease C-terminal (ExoI_SH3)

(212-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 1FXX
  PDB 2QXF
  PDB 3C94
  PDB 3C95
  PDB 3HL8
  PDB 3HP9
  PDB 4HCB
  PDB 4HCC
  PDB 4JRP
  PDB 4JRQ
  PDB 4JS4
  PDB 4JS5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

59.149

98.947

0.585

Multiple sequence alignment