Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   SH08_RS22545 Genome accession   NZ_CP010440
Coordinates   4549325..4549993 (-) Length   222 a.a.
NCBI ID   WP_001350561.1    Uniprot ID   B1X972
Organism   Escherichia coli K-12 strain K-12 MG1655     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4544325..4554993
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SH08_RS22515 (SH08_22530) rfaQ 4544498..4545568 (-) 1071 WP_001350560.1 lipopolysaccharide core heptosyltransferase RfaQ -
  SH08_RS22520 (SH08_22535) waaA 4545974..4547251 (+) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  SH08_RS22525 (SH08_22540) coaD 4547259..4547738 (+) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  SH08_RS22530 (SH08_22545) mutM 4547777..4548586 (-) 810 WP_001114543.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  SH08_RS22535 (SH08_22550) rpmG 4548684..4548851 (-) 168 WP_001051798.1 50S ribosomal protein L33 -
  SH08_RS22540 (SH08_22555) rpmB 4548872..4549108 (-) 237 WP_000091955.1 50S ribosomal protein L28 -
  SH08_RS22545 (SH08_22560) radC 4549325..4549993 (-) 669 WP_001350561.1 DNA repair protein RadC Machinery gene
  SH08_RS22550 (SH08_22565) coaBC 4550165..4551385 (+) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  SH08_RS22555 (SH08_22570) dut 4551363..4551821 (+) 459 WP_000976073.1 dUTP diphosphatase -
  SH08_RS22560 (SH08_22575) slmA 4551928..4552524 (+) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  SH08_RS22565 (SH08_22580) pyrE 4552561..4553202 (-) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  SH08_RS22570 (SH08_22585) rph 4553268..4553984 (-) 717 WP_001247096.1 ribonuclease PH -
  SH08_RS22575 (SH08_22590) yicC 4554111..4554974 (+) 864 WP_000621340.1 YicC/YloC family endoribonuclease -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25343.46 Da        Isoelectric Point: 8.4958

>NTDB_id=120845 SH08_RS22545 WP_001350561.1 4549325..4549993(-) (radC) [Escherichia coli K-12 strain K-12 MG1655]
MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHRRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=120845 SH08_RS22545 WP_001350561.1 4549325..4549993(-) (radC) [Escherichia coli K-12 strain K-12 MG1655]
GTGAAAAACAATTCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGGCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

UPF0758 N-terminal (UPF0758_N)

(9-77)

RadC-like JAB domain (RadC)

(103-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B1X972

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment