Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   SH08_RS20290 Genome accession   NZ_CP010440
Coordinates   4096526..4097959 (+) Length   477 a.a.
NCBI ID   WP_000809057.1    Uniprot ID   P24255
Organism   Escherichia coli K-12 strain K-12 MG1655     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4091526..4102959
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SH08_RS20260 (SH08_20275) yrbG 4092084..4093061 (+) 978 WP_000922901.1 calcium/sodium antiporter -
  SH08_RS20265 (SH08_20280) kdsD 4093075..4094061 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  SH08_RS20270 (SH08_20285) kdsC 4094082..4094648 (+) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  SH08_RS20275 (SH08_20290) lptC 4094645..4095220 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  SH08_RS20280 (SH08_20295) lptA 4095189..4095746 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  SH08_RS20285 (SH08_20300) lptB 4095753..4096478 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  SH08_RS20290 (SH08_20305) rpoN/rpoN1 4096526..4097959 (+) 1434 WP_000809057.1 RNA polymerase factor sigma-54 Machinery gene
  SH08_RS20295 (SH08_20310) hpf 4097982..4098269 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  SH08_RS20300 (SH08_20315) ptsN 4098387..4098878 (+) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  SH08_RS20305 (SH08_20320) rapZ 4098924..4099778 (+) 855 WP_000243741.1 RNase adapter RapZ -
  SH08_RS20310 (SH08_20325) npr 4099775..4100047 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  SH08_RS20315 (SH08_20330) yrbL 4100261..4100893 (+) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  SH08_RS25890 mtgA 4100890..4101015 (-) 126 Protein_3902 monofunctional biosynthetic peptidoglycan transglycosylase -
  SH08_RS20320 (SH08_20335) - 4101161..4102141 (-) 981 WP_000019403.1 IS5-like element IS5 family transposase -
  SH08_RS20325 (SH08_20340) - 4102187..4102388 (+) 202 Protein_3904 YhcE family protein -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53989.78 Da        Isoelectric Point: 4.3663

>NTDB_id=120835 SH08_RS20290 WP_000809057.1 4096526..4097959(+) (rpoN/rpoN1) [Escherichia coli K-12 strain K-12 MG1655]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
ILESIGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=120835 SH08_RS20290 WP_000809057.1 4096526..4097959(+) (rpoN/rpoN1) [Escherichia coli K-12 strain K-12 MG1655]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 2MT3
  PDB 8F1I
  PDB 8F1J
  PDB 8F1K
  PDB 9MSE
  PDB 9MSF
  PDB 9MSG
  PDB 9MSH
  PDB 9MSJ

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.024

100

0.463

Multiple sequence alignment