Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   DI57_RS20945 Genome accession   NZ_CP007546
Coordinates   4426604..4428037 (-) Length   477 a.a.
NCBI ID   WP_023309360.1    Uniprot ID   A0A1Z3N130
Organism   Enterobacter asburiae L1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4421604..4433037
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DI57_RS20915 (DI57_20925) elbB 4423136..4423789 (+) 654 WP_029739612.1 isoprenoid biosynthesis glyoxalase ElbB -
  DI57_RS20920 (DI57_20930) mtgA 4423786..4424511 (+) 726 WP_029739613.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  DI57_RS20925 (DI57_20935) npr 4424551..4424823 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  DI57_RS20930 (DI57_20940) rapZ 4424820..4425674 (-) 855 WP_014885397.1 RNase adapter RapZ -
  DI57_RS20935 (DI57_20945) ptsN 4425720..4426211 (-) 492 WP_029739614.1 PTS IIA-like nitrogen regulatory protein PtsN -
  DI57_RS20940 (DI57_20950) hpf 4426294..4426581 (-) 288 WP_008501444.1 ribosome hibernation promoting factor -
  DI57_RS20945 (DI57_20955) rpoN/rpoN1 4426604..4428037 (-) 1434 WP_023309360.1 RNA polymerase factor sigma-54 Machinery gene
  DI57_RS20950 (DI57_20960) lptB 4428085..4428810 (-) 726 WP_023309359.1 LPS export ABC transporter ATP-binding protein -
  DI57_RS20955 (DI57_20965) lptA 4428817..4429371 (-) 555 WP_023309358.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  DI57_RS20960 (DI57_20970) lptC 4429340..4429915 (-) 576 WP_023309357.1 LPS export ABC transporter periplasmic protein LptC -
  DI57_RS20965 (DI57_20975) kdsC 4429912..4430478 (-) 567 WP_029739615.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  DI57_RS20970 (DI57_20980) kdsD 4430493..4431479 (-) 987 WP_023333606.1 arabinose-5-phosphate isomerase KdsD -
  DI57_RS20975 (DI57_20985) - 4431493..4432470 (-) 978 WP_029739616.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54169.89 Da        Isoelectric Point: 4.3149

>NTDB_id=120771 DI57_RS20945 WP_023309360.1 4426604..4428037(-) (rpoN/rpoN1) [Enterobacter asburiae L1]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDDEIELEEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWIDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=120771 DI57_RS20945 WP_023309360.1 4426604..4428037(-) (rpoN/rpoN1) [Enterobacter asburiae L1]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCAATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
ACTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACTCAGCAGTCACAGGATACAGAAGCGCTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCCGAC
GAGCTACCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACTCCTTCCGGTACGCGTGCAGACTACCAGGATGA
TGAGCTACCGGTCTATCAGGGAGAGACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTGGAACTCACCCCCTTCT
CCGATACCGACCGCGCGATTGCCACCTCAATTGTCGATGCCGTTGACGACACCGGCTATTTGACCGTCACGCTGGACGAT
ATCCTGGAAAGCATGGGCGACGACGAGATTGAGCTTGAGGAAGTCGAAGCCGTACTGAAGCGCATCCAGCGTTTCGATCC
GGTAGGCGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTGATCCAGCTATCTCAGTTCAGCAAAGAGACGCCATGGATCG
ACGAGGCGCGCTTAATCATCAGCGATCATCTGGATCTGTTGGCCAACCACGATTTCCGCTCCCTGATGCGCGTCACGCGC
CTGAAGGAAGAGGTGCTGAAAGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGCCCGGGGCAGTCGATCCAGACCAG
CGAGCCCGAGTACGTGATCCCGGATGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAACTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGCGCGTAACGACTCCGACAATCAATATATTCGC
AGCAATCTTCAGGAAGCGCGATGGTTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTACGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTACTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCATAGTCCACGCGGTATATTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCGTCGTCAACGGCTATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTAG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Z3N130

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment