Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   D082_RS15730 Genome accession   NZ_CP007542
Coordinates   3447591..3448025 (-) Length   144 a.a.
NCBI ID   WP_051738900.1    Uniprot ID   A0A068N0Z6
Organism   Synechocystis sp. PCC 6714     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3442591..3453025
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D082_RS17435 (D082_34750) - 3443481..3445958 (+) 2478 WP_051738898.1 tetratricopeptide repeat protein -
  D082_RS15720 (D082_34760) - 3445983..3446774 (-) 792 WP_028947484.1 hypothetical protein -
  D082_RS15725 (D082_34770) - 3446903..3447289 (-) 387 WP_238546761.1 type IV pilin-like G/H family protein -
  D082_RS19475 - 3447290..3447382 (-) 93 Protein_3172 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  D082_RS15730 (D082_34780) pilA/pilA1 3447591..3448025 (-) 435 WP_051738900.1 type IV pilin protein Machinery gene
  D082_RS19160 (D082_34790) - 3448358..3448606 (-) 249 WP_238546762.1 DUF4351 domain-containing protein -
  D082_RS15740 (D082_34800) - 3448959..3449228 (-) 270 WP_028947486.1 hypothetical protein -
  D082_RS15745 (D082_34810) - 3449432..3450766 (-) 1335 WP_028947487.1 class I SAM-dependent methyltransferase -
  D082_RS15750 (D082_34820) - 3450878..3451990 (-) 1113 WP_028947488.1 hypothetical protein -
  D082_RS15755 (D082_34830) - 3452012..3452901 (-) 890 Protein_3178 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15239.04 Da        Isoelectric Point: 5.0916

>NTDB_id=120749 D082_RS15730 WP_051738900.1 3447591..3448025(-) (pilA/pilA1) [Synechocystis sp. PCC 6714]
MASNFKFKLLSHLTQKKENEGFTLIELLVVVIIIGVLAAIALPNLMNQVGKARNSEGRNGVGALNRAQQVYRTENPTFSS
SIADLDTKVATGEFFTFTSAGNADAATSLATASDPAGQKTTNQTGTVSYDDSTGEFTVTTAFPN

Nucleotide


Download         Length: 435 bp        

>NTDB_id=120749 D082_RS15730 WP_051738900.1 3447591..3448025(-) (pilA/pilA1) [Synechocystis sp. PCC 6714]
ATGGCTAGTAATTTTAAATTCAAACTGCTCTCCCACTTGACCCAGAAGAAAGAAAACGAAGGTTTTACTTTAATTGAACT
TTTGGTTGTTGTAATTATCATCGGTGTGCTGGCGGCTATTGCGCTACCAAACTTGATGAACCAAGTAGGTAAGGCCCGTA
ACTCCGAAGGACGTAACGGTGTAGGTGCCTTAAACCGTGCTCAACAAGTGTATCGTACTGAAAATCCCACGTTTTCTTCG
AGTATCGCTGATCTAGATACAAAGGTTGCTACAGGTGAATTTTTCACCTTTACGAGTGCCGGTAATGCTGATGCAGCAAC
TAGCCTTGCTACAGCCAGTGATCCAGCGGGACAAAAAACCACTAATCAGACTGGTACTGTGAGCTACGATGACAGTACTG
GAGAGTTCACGGTTACAACGGCTTTTCCAAACTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(19-42)

Type IV pilin-like G and H, putative (Pilin_GH)

(55-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A068N0Z6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Synechocystis sp. PCC 6803

54.676

96.528

0.528

Multiple sequence alignment