Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   SH05_RS03660 Genome accession   NZ_CP010438
Coordinates   745451..746833 (+) Length   460 a.a.
NCBI ID   WP_046377642.1    Uniprot ID   -
Organism   Escherichia coli K-12 strain K-12 MG1655     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 740451..751833
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SH05_RS03640 (SH05_03635) yjjJ 741308..742639 (+) 1332 WP_038432747.1 type II toxin-antitoxin system HipA family toxin YjjJ -
  SH05_RS25500 (SH05_03640) lplA 742640..743656 (-) 1017 WP_000105884.1 lipoate--protein ligase LplA -
  SH05_RS25505 (SH05_03645) ytjB 743684..744328 (-) 645 WP_000124615.1 YtjB family periplasmic protein -
  SH05_RS03655 (SH05_03650) serB 744434..745402 (+) 969 WP_046377641.1 phosphoserine phosphatase -
  SH05_RS03660 (SH05_03655) radA/sms 745451..746833 (+) 1383 WP_046377642.1 DNA repair protein RadA Machinery gene
  SH05_RS03665 (SH05_03660) nadR 746854..748086 (+) 1233 WP_000093814.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  SH05_RS26000 (SH05_03665) nadS 748120..748233 (+) 114 Protein_677 NadS family protein -
  SH05_RS03675 (SH05_03670) ettA 748394..750061 (-) 1668 WP_000046749.1 energy-dependent translational throttle protein EttA -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49532.16 Da        Isoelectric Point: 7.1936

>NTDB_id=120627 SH05_RS03660 WP_046377642.1 745451..746833(+) (radA/sms) [Escherichia coli K-12 strain K-12 MG1655]
MAKAPKRTFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPMVARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMTDVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=120627 SH05_RS03660 WP_046377642.1 745451..746833(+) (radA/sms) [Escherichia coli K-12 strain K-12 MG1655]
GTGGCAAAAGCTCCAAAACGCACCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGC
CTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCT
ATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGA
TTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGG
GAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGC
TGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAA
CAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGACGGATGT
ACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGG
CGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCG
GTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCT
GGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAG
TGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTCGATCAC
TCGATGATGGCGAACCCACGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTGTTGCACCG
TCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCG
ACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTC
GGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGC
GATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGC
TTAGCGTGTTCGACGACTTATAA

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(89-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

46.827

99.348

0.465
  radA Streptococcus mitis NCTC 12261

44.079

99.13

0.437
  radA Streptococcus mitis SK321

44.079

99.13

0.437
  radA Streptococcus pneumoniae TIGR4

43.64

99.13

0.433
  radA Streptococcus pneumoniae R6

43.64

99.13

0.433
  radA Streptococcus pneumoniae Rx1

43.64

99.13

0.433
  radA Streptococcus pneumoniae D39

43.64

99.13

0.433

Multiple sequence alignment