Detailed information NTG0120318

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: RR31_RS14425
• Genome accession: NZ_CP010371
• Coordinates: 2920502..2921494 (-)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli strain 6409
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 2915502..2926494

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RR31_RS14405 (RR31_14435)	otnK	2916277..2917539 (+)	1263	WP_000590403.1	3-oxo-tetronate kinase	-
RR31_RS14410 (RR31_14440)	ygbL	2917536..2918174 (+)	639	WP_001278994.1	aldolase	-
RR31_RS14415 (RR31_14445)	ygbM	2918179..2918955 (+)	777	WP_001136934.1	HPr family phosphocarrier protein	-
RR31_RS14420 (RR31_14450)	ygbN	2919044..2920408 (+)	1365	WP_000104441.1	GntP family transporter	-
RR31_RS14425 (RR31_14455)	rpoS	2920502..2921494 (-)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
RR31_RS14430 (RR31_14460)	nlpD	2921557..2922651 (-)	1095	WP_306433997.1	murein hydrolase activator NlpD	-
RR31_RS14435 (RR31_14465)	pcm	2922836..2923462 (-)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
RR31_RS14440 (RR31_14470)	surE	2923456..2924217 (-)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
RR31_RS14445 (RR31_14475)	truD	2924198..2925247 (-)	1050	WP_000568943.1	tRNA pseudouridine(13) synthase TruD	-
RR31_RS14450 (RR31_14480)	ispF	2925244..2925723 (-)	480	WP_001219242.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
RR31_RS14455 (RR31_14485)	ispD	2925723..2926433 (-)	711	WP_000246138.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=120318 RR31_RS14425 WP_000081550.1 2920502..2921494(-) (rpoS) [Escherichia coli strain 6409]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=120318 RR31_RS14425 WP_000081550.1 2920502..2921494(-) (rpoS) [Escherichia coli strain 6409]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715