Detailed information NTG0120041

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: CL55_RS09660
• Genome accession: NZ_CP007501
• Coordinates: 1886491..1887381 (+)
• Length: 296 a.a.
• NCBI ID: WP_046330893.1
• Uniprot ID: A0A0E3ZLB4
• Organism: Polynucleobacter duraquae strain MWH-MoK4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1881491..1892381

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CL55_RS09630 (CL55_00019070)	pth	1881661..1882257 (+)	597	WP_046330887.1	aminoacyl-tRNA hydrolase	-
CL55_RS09635 (CL55_00019080)	-	1882272..1882538 (-)	267	WP_046330888.1	YfhL family 4Fe-4S dicluster ferredoxin	-
CL55_RS09640 (CL55_00019090)	coaD	1882556..1883050 (-)	495	WP_046330889.1	pantetheine-phosphate adenylyltransferase	-
CL55_RS09645 (CL55_00019100)	rsmD	1883087..1883716 (-)	630	WP_335337484.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
CL55_RS09650 (CL55_00019110)	-	1883713..1885053 (-)	1341	WP_046330891.1	M16 family metallopeptidase	-
CL55_RS09655 (CL55_00019120)	-	1885043..1886428 (-)	1386	WP_046330892.1	M16 family metallopeptidase	-
CL55_RS09660 (CL55_00019130)	pilA	1886491..1887381 (+)	891	WP_046330893.1	signal recognition particle-docking protein FtsY	Machinery gene
CL55_RS09665 (CL55_00019140)	rpoH	1887475..1888416 (+)	942	WP_046330894.1	RNA polymerase sigma factor RpoH	-
CL55_RS09670 (CL55_00019150)	-	1888438..1889043 (-)	606	WP_046331301.1	SCO family protein	-
CL55_RS09675 (CL55_00019160)	cyoE	1889051..1889944 (-)	894	WP_046330895.1	heme o synthase	-
CL55_RS09680 (CL55_00019170)	-	1889941..1891071 (-)	1131	WP_082091929.1	COX15/CtaA family protein	-
CL55_RS09685 (CL55_00019180)	-	1891068..1891727 (-)	660	WP_046330896.1	hypothetical protein	-

Sequence

Protein

• Length: 296 a.a.
• Molecular weight: 31634.42 Da
• Isoelectric Point: 6.6106

>NTDB_id=120041 CL55_RS09660 WP_046330893.1 1886491..1887381(+) (pilA) [Polynucleobacter duraquae strain MWH-MoK4]
MFGLRKTLGSLFTSNQTDEAWFDALEESLILSDVGLPTTEQLISKLRKAAKSEKANTPEALKQLLIEEVTILLKSMEPNP
NPLYVHEQKTTPEVWLVVGVNGAGKTTTIGKLCKLFQSQGKSVLLAAGDTFRAAARNQLLEWGGRNQVDVIMQESGDAAA
VAHDAIHAAISRKSDILIIDTAGRLATQDHLMEELKKVKRVIGKALPGAPHQTLLVLDGNTGQNGLNQVRAFHAALELSA
LIVTKLDGTAKGGVICALAHTLSDGPKLAVLALGKGEGIDDLAPFMAAQYSSELFN

Nucleotide

• Length: 891 bp

>NTDB_id=120041 CL55_RS09660 WP_046330893.1 1886491..1887381(+) (pilA) [Polynucleobacter duraquae strain MWH-MoK4]
ATGTTCGGCTTACGTAAAACTCTCGGATCCCTGTTCACATCCAATCAGACTGATGAAGCCTGGTTTGATGCCCTAGAAGA
ATCTCTCATTCTGAGCGATGTGGGTTTGCCCACGACCGAACAATTGATTAGCAAGCTCCGTAAGGCCGCCAAATCGGAAA
AGGCCAATACCCCCGAAGCACTCAAGCAACTACTGATCGAAGAAGTGACGATTCTCCTGAAATCTATGGAGCCCAACCCT
AATCCCTTGTATGTCCATGAGCAAAAGACCACACCAGAGGTATGGCTAGTAGTTGGAGTCAATGGCGCTGGCAAGACCAC
CACGATCGGTAAACTCTGCAAACTTTTTCAATCTCAAGGCAAATCTGTCTTACTCGCAGCCGGTGACACTTTCCGAGCTG
CAGCACGCAACCAACTCCTTGAATGGGGTGGTCGTAATCAGGTCGATGTCATCATGCAAGAAAGTGGTGATGCGGCGGCA
GTGGCACACGATGCCATTCATGCGGCGATTTCTCGCAAGAGCGATATCCTCATTATTGACACTGCCGGCAGATTGGCCAC
TCAAGACCATCTGATGGAAGAATTAAAGAAGGTGAAAAGAGTGATCGGTAAGGCCCTTCCTGGAGCACCTCACCAGACTT
TACTGGTTCTAGATGGCAATACTGGTCAAAACGGTTTAAACCAAGTCAGAGCCTTTCATGCTGCTTTAGAGCTTTCCGCC
TTAATCGTGACTAAATTAGATGGTACCGCTAAGGGCGGGGTAATCTGTGCACTTGCCCACACCCTCAGCGACGGGCCAAA
ACTAGCGGTTTTAGCCTTAGGTAAGGGTGAGGGAATTGATGATTTAGCCCCCTTCATGGCCGCACAATATTCTTCTGAAT
TATTCAATTAA

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (92-295)

• SRP54-type protein, helical bundle domain (SRP54_N) (4-68)

3D structure

• AlphaFold DB: A0A0E3ZLB4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	53.242	98.986	0.527