Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   CD58_RS02000 Genome accession   NZ_CP007410
Coordinates   464643..466718 (+) Length   691 a.a.
NCBI ID   WP_025211417.1    Uniprot ID   -
Organism   Pseudomonas brassicacearum strain DF41     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 459643..471718
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CD58_RS01980 (CD58_02095) comM 461854..462918 (+) 1065 WP_025211413.1 pilus assembly protein PilM Machinery gene
  CD58_RS01985 (CD58_02100) - 462918..463484 (+) 567 WP_025211414.1 PilN domain-containing protein -
  CD58_RS01990 (CD58_02105) pilO 463481..464104 (+) 624 WP_025211415.1 type 4a pilus biogenesis protein PilO -
  CD58_RS01995 (CD58_02110) - 464101..464646 (+) 546 WP_025211416.1 pilus assembly protein PilP -
  CD58_RS02000 (CD58_02115) pilQ 464643..466718 (+) 2076 WP_025211417.1 type IV pilus secretin PilQ Machinery gene
  CD58_RS02005 (CD58_02120) aroK 466723..467241 (+) 519 WP_024779904.1 shikimate kinase AroK -
  CD58_RS02010 (CD58_02125) aroB 467423..468523 (+) 1101 WP_025211418.1 3-dehydroquinate synthase -
  CD58_RS02015 (CD58_02130) - 468534..470126 (+) 1593 WP_025211419.1 AAA family ATPase -

Sequence

Protein


Download         Length: 691 a.a.        Molecular weight: 74739.86 Da        Isoelectric Point: 5.4111

>NTDB_id=119258 CD58_RS02000 WP_025211417.1 464643..466718(+) (pilQ) [Pseudomonas brassicacearum strain DF41]
MNRFLSAFGISLWIALLSPMVHAASLKTLDVAALPGDRVELKLTFDGPPPTPRGYTTDQPARIALDLPGVTNQLTSKSRD
LGGGNARSATVVEAGDRTRLIIGLNQLAPYNTRVEGNNLFVMVGKGAVVPKATAQAARPAVASAAPARVNASVGKAIRGV
DFQRGTQGEGNVVIDLSDPSIAPDIQEREGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNASAGSDRATISIEPSGTF
DYSTYQTDNKLTVSVRPMTVEDLQKRNTDRAAYSGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVP
WDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSAEAKA
DERGTITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNSSGVTSG
TSTTIGTPGSTSTNQPFVDLGASGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQ
EASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQEVPPIKKNEVNAKVLVNDGETIVIGGVFSNT
QSKVVDKVPFLGDVPYLGRLFRRDVVSETKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2076 bp        

>NTDB_id=119258 CD58_RS02000 WP_025211417.1 464643..466718(+) (pilQ) [Pseudomonas brassicacearum strain DF41]
ATGAACAGGTTTTTGTCAGCCTTCGGTATTTCGCTATGGATAGCGCTGTTGTCGCCGATGGTTCACGCGGCCAGTCTGAA
GACGCTGGATGTCGCGGCGTTGCCGGGTGACCGCGTCGAGTTGAAGCTCACCTTCGACGGGCCGCCGCCCACGCCCCGGG
GCTACACCACCGATCAACCGGCCCGGATCGCACTGGATCTGCCCGGCGTGACCAATCAACTGACCAGCAAGAGCCGCGAC
CTGGGGGGCGGCAATGCGCGCAGCGCCACAGTGGTCGAGGCGGGCGACCGCACGCGGTTGATCATCGGCCTTAACCAATT
GGCGCCGTATAACACCCGGGTCGAGGGTAATAACCTGTTCGTGATGGTTGGCAAGGGCGCCGTAGTGCCCAAGGCCACTG
CCCAGGCTGCGCGCCCGGCGGTTGCCAGCGCCGCGCCTGCCAGGGTCAATGCATCGGTGGGCAAGGCTATCCGTGGCGTG
GATTTCCAGCGCGGGACCCAGGGTGAAGGCAATGTGGTGATCGATCTGTCGGACCCTTCCATCGCCCCGGACATCCAGGA
ACGCGAAGGCAAGATCATCCTCAATTTCGCCAGGACCCAGTTGCCCGAACCGCTGCGGGTGCGCCTGGACGTCAAGGACT
TCGCCACCCCGGTGCAATTCGTCAATGCCAGTGCGGGTAGCGACCGAGCGACGATCAGCATCGAGCCGAGCGGCACCTTC
GATTACTCGACCTACCAGACCGACAACAAGCTGACCGTCAGCGTCCGGCCCATGACCGTCGAGGATCTGCAGAAACGCAA
TACCGATCGCGCCGCCTACAGCGGTGAAAAACTGTCCCTGAACTTCCAGGACATCGAGGTGCGTTCGGTCCTGCAACTGA
TCGCCGACTTCACCAACCTGAACCTGGTGGCCAGCGACACCGTGCAGGGTGGCATCACCCTACGCTTGCAGAACGTGCCT
TGGGATCAGGCGCTGGACCTCGTGCTGAAGACCAAAGGGTTGGACAAACGCAAGGTTGGCAACGTGCTGCTGGTCGCCCC
GGCCGATGAAATTGCCGCGCGCGAGCGCCAGGAGCTGGAGTCCCAGAAGCAGATTGCCGACCTGGCGCCGTTGCGGCGTG
AGTTGTTGCAGGTCAACTACGCGAAGGCGGCGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGTGCCGAAGCGAAAGCC
GATGAGCGCGGCACGATCACGGTGGATGAGCGAACCAACAACATCATTGCCTACCAGACCCAGGACCGGCTCGACGAGCT
GCGCCGGATCGTGGCGCAGCTGGATATTCCAGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCGATT
ACGACAAGAGCCTGGGTGTGCGCTGGGGCGGTTCGATACAAAACAAGGGCAACTGGAACTCCTCGGGGGTGACCAGCGGC
ACCTCCACGACCATTGGCACGCCGGGCAGCACCAGCACCAACCAGCCGTTCGTCGATCTGGGCGCGTCGGGCAATACTTC
CGGGATCGGTATCGCGTTCATTACTGACAACGTCTTGCTGGACCTGGAGCTGACGGCCATGGAGAAAACCGGCAACGGCG
AGATCGTCTCCCAGCCCAAGGTCGTGACCTCCGACAAGGAGACGGCAAAGATCCTCAAGGGCACGGAGATTCCTTATCAG
GAAGCCAGCTCCAGCGGTGCCACGTCGGTGTCGTTCAAGGAGGCCTCGCTGTCGCTGGAAGTGACCCCGCAGATCACCCC
GGACAATCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACCTGAACAAAGTGCAGGAAGTGCCACCGA
TCAAGAAAAACGAAGTCAACGCCAAGGTCCTGGTCAACGATGGCGAGACCATTGTGATTGGCGGGGTTTTCTCAAATACT
CAGAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGTTTC
GGAGACAAAATCCGAGCTGCTGGTGTTCCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(375-440)

Bacterial type II and III secretion system protein (Secretin)

(526-682)

AMIN domain (AMIN)

(38-121)

Secretin and TonB N terminus short domain (STN)

(301-347)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.287

100

0.758
  pilQ Acinetobacter baumannii strain A118

38.71

100

0.417
  pilQ Acinetobacter baumannii D1279779

38.71

100

0.417
  comQ Acinetobacter baylyi ADP1

41.534

100

0.415
  pilQ Legionella pneumophila strain Lp02

38.815

97.685

0.379
  pilQ Legionella pneumophila strain ERS1305867

38.815

97.685

0.379

Multiple sequence alignment