Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   Q433_RS13045 Genome accession   NZ_CP007409
Coordinates   2505350..2505760 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis subsp. subtilis str. OH 131.1 strain OH131.1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2500350..2510760
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q433_RS13020 (Q433_14065) yqeF 2500919..2501650 (-) 732 WP_029726702.1 SGNH/GDSL hydrolase family protein -
  Q433_RS13025 (Q433_14075) cwlH 2501902..2502594 (-) 693 WP_051673217.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  Q433_RS13030 (Q433_14080) yqeD 2502840..2503466 (+) 627 WP_038427798.1 TVP38/TMEM64 family protein -
  Q433_RS13035 (Q433_14085) gnd 2503485..2504353 (-) 869 Protein_2516 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  Q433_RS13040 (Q433_14090) yqeB 2504595..2505317 (+) 723 WP_010886572.1 hypothetical protein -
  Q433_RS13045 (Q433_14095) nucA/comI 2505350..2505760 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  Q433_RS13050 (Q433_14105) sigK 2505955..2506683 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  Q433_RS21965 - 2506683..2506748 (+) 66 Protein_2520 hypothetical protein -
  Q433_RS13060 (Q433_14115) yrkQ 2506777..2508075 (-) 1299 WP_003246194.1 two-component system sensor histidine kinase YrkQ -
  Q433_RS13065 (Q433_14120) yrkP 2508062..2508757 (-) 696 WP_003229895.1 two-component response regulator YrkP -
  Q433_RS13070 (Q433_14125) yrkO 2509025..2510242 (+) 1218 WP_015251643.1 DUF418 domain-containing protein -
  Q433_RS21605 - 2510161..2510409 (+) 249 WP_119123070.1 hypothetical protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=119230 Q433_RS13045 WP_009967785.1 2505350..2505760(-) (nucA/comI) [Bacillus subtilis subsp. subtilis str. OH 131.1 strain OH131.1]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=119230 Q433_RS13045 WP_009967785.1 2505350..2505760(-) (nucA/comI) [Bacillus subtilis subsp. subtilis str. OH 131.1 strain OH131.1]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGATAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCTGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment