Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RG73_RS10690 Genome accession   NZ_CP010242
Coordinates   2046394..2046834 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain S56     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2041394..2051834
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG73_RS10670 (RG73_10105) coaE 2041884..2042504 (-) 621 WP_074014601.1 dephospho-CoA kinase -
  RG73_RS27705 - 2042529..2042573 (+) 45 WP_120795372.1 protein YacM -
  RG73_RS10675 (RG73_10110) guaC 2042729..2043772 (+) 1044 WP_001217338.1 GMP reductase -
  RG73_RS10680 (RG73_10115) hofC 2043807..2045009 (-) 1203 WP_000157234.1 protein transport protein HofC -
  RG73_RS10685 (RG73_10120) pilB 2044999..2046384 (-) 1386 WP_001025149.1 type II secretion system protein GspE Machinery gene
  RG73_RS10690 (RG73_10125) pilA 2046394..2046834 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  RG73_RS10700 (RG73_10135) nadC 2047037..2047930 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RG73_RS10705 (RG73_10140) ampD 2048018..2048569 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RG73_RS10710 (RG73_10145) ampE 2048566..2049420 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  RG73_RS10715 (RG73_10150) aroP 2049463..2050836 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=118943 RG73_RS10690 WP_000360904.1 2046394..2046834(-) (pilA) [Escherichia coli strain S56]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=118943 RG73_RS10690 WP_000360904.1 2046394..2046834(-) (pilA) [Escherichia coli strain S56]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363