Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   RG66_RS10180 Genome accession   NZ_CP010229
Coordinates   2014836..2015513 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain S10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2009836..2020513
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG66_RS10165 (RG66_09750) chiA 2011203..2013896 (+) 2694 WP_001402584.1 bifunctional chitinase/lysozyme -
  RG66_RS10170 (RG66_09755) bfd 2014065..2014259 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  RG66_RS10175 (RG66_09760) bfr 2014331..2014807 (+) 477 WP_000675504.1 bacterioferritin -
  RG66_RS10180 (RG66_09765) pilD 2014836..2015513 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  RG66_RS10185 (RG66_09770) gspM 2015513..2015974 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  RG66_RS10190 (RG66_09775) gspL 2015971..2017134 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  RG66_RS10195 (RG66_09780) gspK 2017149..2018132 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  RG66_RS10200 (RG66_09785) gspJ 2018125..2018712 (-) 588 WP_001402582.1 type II secretion system minor pseudopilin GspJ -
  RG66_RS10205 (RG66_09790) gspI 2018705..2019082 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  RG66_RS10210 (RG66_09795) gspH 2019079..2019588 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  RG66_RS25445 (RG66_09800) gspG 2019596..2020033 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=118367 RG66_RS10180 WP_000178161.1 2014836..2015513(-) (pilD) [Escherichia coli strain S10]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=118367 RG66_RS10180 WP_000178161.1 2014836..2015513(-) (pilD) [Escherichia coli strain S10]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAACTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment