Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   RG64_RS25330 Genome accession   NZ_CP010226
Coordinates   4659564..4660241 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain S1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4654564..4665241
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG64_RS28115 (RG64_23200) - 4655069..4655344 (+) 276 WP_000179213.1 IS1-like element transposase -
  RG64_RS28120 - 4655389..4655752 (+) 364 Protein_4534 IS1 family transposase -
  RG64_RS25315 (RG64_23205) chiA 4655931..4658624 (+) 2694 WP_016241662.1 bifunctional chitinase/lysozyme -
  RG64_RS25320 (RG64_23210) bfd 4658793..4658987 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  RG64_RS25325 (RG64_23215) bfr 4659059..4659535 (+) 477 WP_000675504.1 bacterioferritin -
  RG64_RS25330 (RG64_23220) pilD 4659564..4660241 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  RG64_RS25335 (RG64_23225) gspM 4660241..4660702 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  RG64_RS25340 (RG64_23230) gspL 4660699..4661862 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  RG64_RS25345 (RG64_23235) gspK 4661877..4662860 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  RG64_RS25350 (RG64_23240) gspJ 4662853..4663440 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  RG64_RS25355 (RG64_23245) gspI 4663433..4663810 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  RG64_RS25360 (RG64_23250) gspH 4663807..4664316 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  RG64_RS26460 (RG64_23255) gspG 4664324..4664761 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=118250 RG64_RS25330 WP_000178161.1 4659564..4660241(-) (pilD) [Escherichia coli strain S1]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=118250 RG64_RS25330 WP_000178161.1 4659564..4660241(-) (pilD) [Escherichia coli strain S1]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment