Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   KHU1_RS16355 Genome accession   NZ_CP007242
Coordinates   3419958..3421103 (+) Length   381 a.a.
NCBI ID   WP_007407638.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens KHG19     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3414958..3426103
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHU1_RS16335 (KHU1_3192) - 3415855..3416283 (-) 429 WP_007407634.1 DUF1934 domain-containing protein -
  KHU1_RS16340 (KHU1_3193) - 3416437..3416949 (+) 513 Protein_3267 bacteriocin biosynthesis protein AlbG -
  KHU1_RS16345 (KHU1_3194) - 3416956..3418332 (-) 1377 WP_042635568.1 YncE family protein -
  KHU1_RS16350 (KHU1_3195) - 3418372..3419727 (-) 1356 WP_042635569.1 YncE family protein -
  KHU1_RS16355 (KHU1_3196) rapF 3419958..3421103 (+) 1146 WP_007407638.1 tetratricopeptide repeat protein Regulator
  KHU1_RS16360 (KHU1_3197) phrF 3421087..3421206 (+) 120 WP_041482476.1 PhrC/PhrF family phosphatase-inhibitory pheromone Regulator
  KHU1_RS16365 (KHU1_3198) - 3421404..3421607 (+) 204 WP_007407640.1 helix-turn-helix transcriptional regulator -
  KHU1_RS16370 (KHU1_3199) - 3421621..3421824 (+) 204 WP_007407641.1 hypothetical protein -
  KHU1_RS16375 (KHU1_3200) - 3421859..3422044 (+) 186 WP_007407642.1 DUF4177 domain-containing protein -
  KHU1_RS16380 (KHU1_3201) - 3422230..3422511 (+) 282 WP_007407643.1 hypothetical protein -
  KHU1_RS16385 (KHU1_3202) - 3422555..3423067 (+) 513 WP_042635570.1 stage II sporulation protein M -
  KHU1_RS16395 (KHU1_3204) - 3423658..3423876 (-) 219 WP_003151056.1 helix-turn-helix transcriptional regulator -
  KHU1_RS16400 (KHU1_3205) speB 3424044..3424916 (-) 873 WP_007614448.1 agmatinase -
  KHU1_RS16405 (KHU1_3206) speE 3424975..3425805 (-) 831 WP_042635571.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45543.57 Da        Isoelectric Point: 4.5769

>NTDB_id=118175 KHU1_RS16355 WP_007407638.1 3419958..3421103(+) (rapF) [Bacillus amyloliquefaciens KHG19]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAEYKLDFVKDCIEKAEFYFKMSESYYYMKQTYFS
MDYARQAYKIYHKQEAYNIRVLQCHSLFATNFLDLKQYDEAIQHFKKAYAMAEAEQQPQLMGRTLYNIGLCFNSQENYKP
AIDYIKRAIAVFEDGNIITSLPQAYFLITQIHYKIGNMAIARQYHDKGVSYAEEAEDSLYIAEYEFLESLYVGEPDEEAI
MECFDFLRDKSMYADLEDFALDVAKYYHERENFEKASAYFLKVEETRQQIQGGVKLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=118175 KHU1_RS16355 WP_007407638.1 3419958..3421103(+) (rapF) [Bacillus amyloliquefaciens KHG19]
GTGGCGAGTGTTTTATCATCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGTCTGGAAGATGATCAGGATCTTCATCTATACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCAGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGTCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAATATAAGCTTGATTTTG
TCAAAGATTGTATCGAGAAAGCTGAATTTTATTTTAAGATGTCAGAGTCCTACTATTATATGAAACAGACTTATTTTTCT
ATGGATTATGCCCGGCAGGCTTATAAAATTTATCATAAGCAGGAGGCATATAATATAAGGGTGCTTCAGTGCCATTCATT
GTTCGCTACCAACTTTCTTGACTTAAAGCAATATGATGAAGCAATTCAGCATTTTAAAAAGGCTTATGCCATGGCAGAGG
CAGAACAGCAGCCACAATTAATGGGCCGGACTCTATATAATATCGGACTTTGTTTTAACAGCCAAGAAAATTACAAACCA
GCCATTGATTACATAAAACGGGCAATAGCTGTTTTTGAAGATGGGAACATCATCACTTCTCTCCCGCAAGCCTATTTCTT
AATCACACAAATACATTATAAAATCGGAAATATGGCCATAGCCAGACAATATCATGACAAAGGGGTATCTTATGCAGAGG
AGGCAGAAGATTCCCTATATATCGCCGAGTATGAATTTTTAGAATCCTTGTATGTCGGTGAGCCTGATGAAGAAGCAATC
ATGGAATGTTTTGACTTTCTCAGAGATAAATCGATGTATGCTGATCTTGAAGATTTTGCCTTAGATGTGGCAAAATATTA
TCATGAAAGAGAAAATTTCGAGAAGGCTTCCGCATATTTTTTAAAGGTGGAAGAAACAAGACAGCAGATTCAAGGAGGTG
TGAAGTTGTATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_12)

(189-253)

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

78.478

100

0.785
  rapC Bacillus subtilis subsp. subtilis str. 168

56.579

99.738

0.564

Multiple sequence alignment