Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   RG59_RS10540 Genome accession   NZ_CP010200
Coordinates   2006522..2006989 (+) Length   155 a.a.
NCBI ID   WP_000340170.1    Uniprot ID   -
Organism   Escherichia coli strain M10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2001522..2011989
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG59_RS10490 (RG59_10090) rplP 2001632..2002042 (-) 411 WP_000941212.1 50S ribosomal protein L16 -
  RG59_RS10495 (RG59_10095) rpsC 2002055..2002756 (-) 702 WP_000529945.1 30S ribosomal protein S3 -
  RG59_RS10500 (RG59_10100) rplV 2002774..2003106 (-) 333 WP_000447529.1 50S ribosomal protein L22 -
  RG59_RS10505 (RG59_10105) rpsS 2003121..2003399 (-) 279 WP_001138117.1 30S ribosomal protein S19 -
  RG59_RS10510 (RG59_10110) rplB 2003416..2004237 (-) 822 WP_000301864.1 50S ribosomal protein L2 -
  RG59_RS10515 (RG59_10115) rplW 2004255..2004557 (-) 303 WP_000617544.1 50S ribosomal protein L23 -
  RG59_RS10520 (RG59_10120) rplD 2004554..2005159 (-) 606 WP_000424395.1 50S ribosomal protein L4 -
  RG59_RS10525 (RG59_10125) rplC 2005170..2005799 (-) 630 WP_000579833.1 50S ribosomal protein L3 -
  RG59_RS10530 (RG59_10130) rpsJ 2005832..2006143 (-) 312 WP_001181004.1 30S ribosomal protein S10 -
  RG59_RS10540 (RG59_10140) pilD 2006522..2006989 (+) 468 WP_000340170.1 prepilin peptidase Machinery gene
  RG59_RS10545 (RG59_10145) bfr 2006986..2007462 (-) 477 WP_000675515.1 bacterioferritin -
  RG59_RS10550 (RG59_10150) bfd 2007535..2007729 (-) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  RG59_RS10560 (RG59_10155) tuf 2007912..2009096 (-) 1185 WP_000031783.1 elongation factor Tu -
  RG59_RS10565 (RG59_10160) fusA 2009167..2011281 (-) 2115 WP_000124700.1 elongation factor G -
  RG59_RS10570 (RG59_10165) rpsG 2011378..2011848 (-) 471 WP_001138043.1 30S ribosomal protein S7 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16934.09 Da        Isoelectric Point: 8.1296

>NTDB_id=117795 RG59_RS10540 WP_000340170.1 2006522..2006989(+) (pilD) [Escherichia coli strain M10]
MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWPFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=117795 RG59_RS10540 WP_000340170.1 2006522..2006989(+) (pilD) [Escherichia coli strain M10]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGCCCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361
  pilD Neisseria gonorrhoeae MS11

38.095

94.839

0.361