Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RG55_RS06390 Genome accession   NZ_CP010183
Coordinates   1198875..1199315 (+) Length   146 a.a.
NCBI ID   WP_000360927.1    Uniprot ID   -
Organism   Escherichia coli strain M3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1193875..1204315
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG55_RS06365 (RG55_06025) aroP 1194873..1196246 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  RG55_RS06370 (RG55_06030) ampE 1196289..1197143 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  RG55_RS06375 (RG55_06035) ampD 1197140..1197691 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RG55_RS06380 (RG55_06040) nadC 1197779..1198672 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RG55_RS06390 (RG55_06050) pilA 1198875..1199315 (+) 441 WP_000360927.1 prepilin peptidase-dependent pilin Machinery gene
  RG55_RS06395 (RG55_06055) pilB 1199325..1200710 (+) 1386 WP_001025170.1 type II secretion system protein GspE Machinery gene
  RG55_RS06400 (RG55_06060) hofC 1200700..1201902 (+) 1203 WP_000157236.1 protein transport protein HofC -
  RG55_RS06405 (RG55_06065) guaC 1201937..1202980 (-) 1044 WP_001217338.1 GMP reductase -
  RG55_RS28335 - 1203136..1203180 (-) 45 WP_120795372.1 protein YacM -
  RG55_RS06410 (RG55_06070) coaE 1203205..1203825 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15533.60 Da        Isoelectric Point: 4.2529

>NTDB_id=117448 RG55_RS06390 WP_000360927.1 1198875..1199315(+) (pilA) [Escherichia coli strain M3]
MDKQSGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=117448 RG55_RS06390 WP_000360927.1 1198875..1199315(+) (pilA) [Escherichia coli strain M3]
ATGGACAAGCAAAGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

42.742

84.932

0.363

Multiple sequence alignment