Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   BD33_RS05660 Genome accession   NZ_CP007193
Coordinates   1082651..1083610 (+) Length   319 a.a.
NCBI ID   WP_002875425.1    Uniprot ID   -
Organism   Campylobacter jejuni strain NS4-9-1     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1062945..1087653 1082651..1083610 within 0


Gene organization within MGE regions


Location: 1062945..1087653
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BD33_RS05570 (BD33_05610) pglI 1062945..1063874 (-) 930 WP_002852770.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  BD33_RS05575 (BD33_05615) pglH 1063867..1064946 (-) 1080 WP_010891911.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  BD33_RS05580 (BD33_05620) pglK 1064943..1066637 (-) 1695 WP_002858308.1 ABC-type lipopolysaccharide transporter PglK -
  BD33_RS05585 (BD33_05625) galE 1066631..1067617 (-) 987 WP_002858414.1 UDP-glucose 4-epimerase GalE -
  BD33_RS05590 (BD33_05630) - 1067675..1068469 (-) 795 WP_002853812.1 3'-5' exonuclease -
  BD33_RS05595 (BD33_05635) waaC 1068535..1069563 (+) 1029 WP_002858077.1 lipopolysaccharide heptosyltransferase I -
  BD33_RS05600 (BD33_05640) htrB 1069553..1070440 (+) 888 WP_002858051.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  BD33_RS05605 (BD33_05645) - 1070437..1071984 (+) 1548 WP_002858148.1 glycosyltransferase -
  BD33_RS05610 (BD33_05650) - 1071988..1073160 (+) 1173 WP_002858013.1 glycosyltransferase family 2 protein -
  BD33_RS05615 (BD33_05655) - 1073144..1074139 (-) 996 WP_002858358.1 capsular polysaccharide synthesis protein -
  BD33_RS05620 (BD33_05660) - 1074197..1075366 (+) 1170 WP_002864814.1 glycosyltransferase family 2 protein -
  BD33_RS05625 (BD33_05665) wlaN 1075355..1076266 (-) 912 WP_002858039.1 beta-1,3 galactosyltransferase -
  BD33_RS05630 (BD33_05670) cstIII 1076321..1077205 (+) 885 WP_002858290.1 alpha-2,3-sialyltransferase -
  BD33_RS05635 (BD33_05675) neuB1 1077186..1078217 (+) 1032 WP_002858213.1 sialic acid synthase -
  BD33_RS05640 (BD33_05680) neuC 1078214..1079329 (+) 1116 WP_002858400.1 UDP-N-acetylglucosamine 2-epimerase -
  BD33_RS08965 (BD33_05685) - 1079326..1080240 (+) 915 Protein_1089 beta-1,4-N-acetylgalactosaminyltransferase -
  BD33_RS08970 (BD33_05690) - 1080274..1080936 (+) 663 WP_002881429.1 N-acylneuraminate cytidylyltransferase -
  BD33_RS05650 (BD33_05695) - 1080931..1081784 (-) 854 Protein_1091 glycosyltransferase -
  BD33_RS05655 (BD33_05700) waaV 1081768..1082592 (-) 825 WP_002858096.1 glycosyltransferase family 2 protein -
  BD33_RS05660 (BD33_05705) waaF 1082651..1083610 (+) 960 WP_002875425.1 lipopolysaccharide heptosyltransferase II Regulator
  BD33_RS05665 (BD33_05710) gmhA 1083591..1084151 (-) 561 WP_002858021.1 D-sedoheptulose 7-phosphate isomerase -
  BD33_RS05670 (BD33_05715) rfaE1 1084148..1085533 (-) 1386 WP_052778309.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  BD33_RS05675 (BD33_05720) rfaD 1085526..1086479 (-) 954 WP_002852832.1 ADP-glyceromanno-heptose 6-epimerase -
  BD33_RS05680 (BD33_05725) gmhB 1086480..1087040 (-) 561 WP_002878815.1 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  BD33_RS05685 (BD33_05730) - 1087122..1087424 (+) 303 WP_002852762.1 cytochrome c -
  BD33_RS05690 (BD33_05735) ccoS 1087447..1087653 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -

Sequence

Protein


Download         Length: 319 a.a.        Molecular weight: 36734.30 Da        Isoelectric Point: 10.2412

>NTDB_id=117370 BD33_RS05660 WP_002875425.1 1082651..1083610(+) (waaF) [Campylobacter jejuni strain NS4-9-1]
MKIFIHLPTWLGDTVMASPALYTIKEHFKDAQFILYGSFVSTALFKEFPNSKIIIENKLSRYKQALSLRKELGKIDLSFA
FRSAFSSKIILHILKTKQRYFFDKNKYKEEHQVLKYLYFIENSLSIKAHFKDLKLPFKLKFQNPLILRNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHDILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHLSAVYKVKTVAIFGPTKFTQTSPWQNENAKLVHLDLACMPCMQKTCPLKHHKCMKDLKPEKVIEQAKNLLKNSHL

Nucleotide


Download         Length: 960 bp        

>NTDB_id=117370 BD33_RS05660 WP_002875425.1 1082651..1083610(+) (waaF) [Campylobacter jejuni strain NS4-9-1]
ATGAAAATTTTTATACATCTTCCCACCTGGTTAGGCGATACGGTAATGGCTTCACCTGCTTTATACACTATAAAAGAACA
TTTTAAAGATGCTCAGTTTATCCTTTATGGTTCTTTTGTTTCTACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACTATCCCGTTATAAACAAGCTCTATCTTTACGCAAAGAACTTGGTAAAATCGATCTTAGCTTTGCT
TTTAGATCTGCGTTCTCTTCTAAGATTATCTTGCATATCCTTAAAACAAAACAAAGATATTTTTTTGACAAAAACAAGTA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAATTCACTTAGTATAAAAGCTCATTTTAAGGACTTAA
AGCTTCCCTTTAAGCTAAAATTTCAAAACCCTCTTATCTTAAGAAATGGTAAAAAAATTCTAGGACTCAACCCTGGTGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGATATTTT
AATCTTTGGTGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACCACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGACCTATGCACCTAAGTGCGGTTTATAAGGTAAAAACCGTAGCTATTTTTGGCCCTACGAAATTTACTCAAACTTCACC
TTGGCAAAATGAAAATGCAAAATTAGTGCATTTAGATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATCTAAAGCCTGAAAAAGTGATAGAACAGGCTAAAAATTTACTTAAAAACTCTCATCTATGA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(94-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

100

100

1

Multiple sequence alignment