Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   AD53_RS06490 Genome accession   NZ_CP007188
Coordinates   1194954..1195913 (+) Length   319 a.a.
NCBI ID   WP_002852903.1    Uniprot ID   A0A5Y6P8U5
Organism   Campylobacter jejuni strain HF5-4A-4     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1177270..1199956 1194954..1195913 within 0


Gene organization within MGE regions


Location: 1177270..1199956
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AD53_RS06405 (AD53_06435) pglI 1177270..1178199 (-) 930 WP_002852770.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  AD53_RS06410 (AD53_06440) pglH 1178192..1179271 (-) 1080 WP_075912956.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  AD53_RS06415 (AD53_06445) pglK 1179268..1180962 (-) 1695 WP_052834644.1 ABC-type lipopolysaccharide transporter PglK -
  AD53_RS06420 (AD53_06450) galE 1180956..1181942 (-) 987 WP_002852683.1 UDP-glucose 4-epimerase GalE -
  AD53_RS06425 (AD53_06455) - 1182000..1182794 (-) 795 WP_002852820.1 3'-5' exonuclease -
  AD53_RS06430 (AD53_06460) waaC 1182861..1183889 (+) 1029 WP_002852873.1 lipopolysaccharide heptosyltransferase I -
  AD53_RS06435 (AD53_06465) htrB 1183879..1184766 (+) 888 WP_002852805.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  AD53_RS06440 (AD53_06470) - 1184763..1186311 (+) 1549 Protein_1251 glycosyltransferase -
  AD53_RS06445 (AD53_06480) - 1186315..1187484 (+) 1170 WP_002852863.1 glycosyltransferase family 2 protein -
  AD53_RS06450 (AD53_06485) - 1187554..1188597 (+) 1044 WP_002870498.1 beta-1,4-N-acetylgalactosaminyltransferase -
  AD53_RS06455 (AD53_06490) - 1188583..1189488 (-) 906 WP_075912957.1 glycosyltransferase family 2 protein -
  AD53_RS06460 (AD53_06495) - 1189577..1190452 (+) 876 WP_002878823.1 alpha-2,3-sialyltransferase -
  AD53_RS06465 (AD53_06500) neuB 1190453..1191493 (+) 1041 WP_002924069.1 N-acetylneuraminate synthase -
  AD53_RS06470 (AD53_06505) neuC 1191490..1192608 (+) 1119 WP_002878821.1 UDP-N-acetylglucosamine 2-epimerase -
  AD53_RS06475 (AD53_06510) - 1192605..1193270 (+) 666 WP_002878820.1 acylneuraminate cytidylyltransferase family protein -
  AD53_RS06480 (AD53_06515) - 1193258..1194091 (+) 834 WP_002878819.1 CatB-related O-acetyltransferase -
  AD53_RS06485 (AD53_06520) waaV 1194083..1194895 (-) 813 WP_002878817.1 glycosyltransferase family 2 protein -
  AD53_RS06490 (AD53_06525) waaF 1194954..1195913 (+) 960 WP_002852903.1 lipopolysaccharide heptosyltransferase II Regulator
  AD53_RS06495 (AD53_06530) gmhA 1195894..1196454 (-) 561 WP_002852775.1 D-sedoheptulose 7-phosphate isomerase -
  AD53_RS06500 (AD53_06535) rfaE1 1196451..1197836 (-) 1386 WP_002852777.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  AD53_RS06505 (AD53_06540) rfaD 1197829..1198782 (-) 954 WP_002852832.1 ADP-glyceromanno-heptose 6-epimerase -
  AD53_RS06510 (AD53_06545) gmhB 1198783..1199343 (-) 561 WP_002878815.1 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  AD53_RS06515 (AD53_06550) - 1199425..1199727 (+) 303 WP_002852762.1 cytochrome c -
  AD53_RS06520 (AD53_06555) ccoS 1199750..1199956 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -

Sequence

Protein


Download         Length: 319 a.a.        Molecular weight: 36766.44 Da        Isoelectric Point: 10.4054

>NTDB_id=117235 AD53_RS06490 WP_002852903.1 1194954..1195913(+) (waaF) [Campylobacter jejuni strain HF5-4A-4]
MKIFIHLPTWLGDAVMASPALYTIKEHFKNAQFILYGSLVSTALFKEFPNSKIIIENKQSRYKQALSLRKKLGKIDFSFA
FRSAFSSKIILHILKTKQRYFFDKNKYKEEHQVLKYLYFIENSLSIKAHFKDLKLPFKLKFQNPLVLKNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHEILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHLSAVYKVKTVAIFGPTKFTQTSPWQNQNAKLVHLDLACMPCMQKICPLKHHKCMKDLKPQRVIEEARNLLKNPHL

Nucleotide


Download         Length: 960 bp        

>NTDB_id=117235 AD53_RS06490 WP_002852903.1 1194954..1195913(+) (waaF) [Campylobacter jejuni strain HF5-4A-4]
ATGAAAATTTTTATACACCTTCCCACTTGGCTAGGCGATGCGGTGATGGCTTCACCTGCTTTATACACCATAAAAGAACA
TTTTAAAAATGCTCAGTTTATCCTTTATGGATCTTTGGTTTCCACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACAATCCCGCTATAAACAAGCCCTATCTTTACGCAAAAAACTTGGTAAAATCGATTTTAGCTTTGCT
TTTAGATCTGCGTTTTCTTCTAAGATTATCTTGCATATTCTTAAAACAAAACAAAGATATTTTTTTGACAAAAACAAGTA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTAGTATAAAAGCTCATTTTAAAGACTTAA
AACTTCCCTTTAAGCTAAAATTTCAAAACCCTCTTGTCTTAAAAAATGGCAAAAAAATTCTAGGACTCAACCCTGGCGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGAGATTTT
AATCTTTGGTGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACCACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGTCCTATGCACCTAAGTGCGGTTTATAAGGTAAAAACCGTAGCTATTTTTGGCCCCACAAAATTTACCCAAACTTCACC
TTGGCAAAATCAAAACGCAAAATTAGTACACTTAGATCTAGCTTGTATGCCTTGTATGCAAAAAATCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATTTAAAGCCTCAAAGGGTTATAGAAGAAGCTAGAAATTTACTTAAAAACCCTCATCTATAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(96-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5Y6P8U5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

94.984

100

0.95

Multiple sequence alignment